SRR5940794 - Chrysomya megacephala

Basic Information

Run: SRR5940794

Assay Type: WGS

Bioproject: PRJNA385554

Biosample: SAMN07135677

Bytes: 2228668116

Center Name: NANYANG TECHNOLOGICAL UNIVERSITY

Sequencing Information

Instrument: Illumina HiSeq 2500

Library Layout: PAIRED

Library Selection: RANDOM

Platform: ILLUMINA

Geographic Information

Country: Brazil

Continent: South America

Location Name: Brazil: Campinas

Latitude/Longitude: 22.8272 S 47.0636 W

Sample Information

Host: Chrysomya megacephala

Isolation: hospital

Biosample Model: Metagenome or environmental

Collection Date: 2012-05-16

Taxonomic Classification

Potential Symbionts

About Potential Symbionts

This table shows potential symbiont identified in the metagenome sample. Matches are scored based on:

Relative abundance in the sample
Species-level matches with known symbionts
Host insect order matches with reference records
Completeness and richness of functional records

Note: only the three highest scoring comparison records were retained for each symbiont species or genus in the sample

Based on our current records database, this section aims to identify potential functional symbionts in this metagenome sample, with scoring based on:

Relative abundance in sample
Species-level matches with known symbionts
Host insect order matches
Functional record completeness

Note: Showing top 3 highest scoring records for each species/genus

Symbiont Name	Record	Host Species	Function	Abundance	Score Score Composition: Symbiont Abundance Species match bonus Host match bonus Function richness Higher scores indicate stronger symbiotic relationship potential
Ignatzschineria Host Order Match Host Species Match	RISB0562	Chrysomya megacephala Order: Diptera	Ignatzschineria indica is a Gram-negative bacterium commonly associated with maggot infestation and myiasis, a probable marker for myiasis diagnosis	0.02%	33.0
Wolbachia pipientis Species-level Match Host Order Match	RISB0766	Aedes fluviatilis Order: Diptera	The presence of Wolbachia pipientis improves energy performance in A. fluviatilis cells; it affects the regulation of key energy sources such as lipids, proteins, and carbohydrates, making the distribution of actin more peripheral and with extensions that come into contact with neighboring cells.	1.23%	21.2
Lactococcus lactis Species-level Match Host Order Match	RISB0131	Ceratitis capitata Order: Diptera	The intestinal microbiota structure was significantly influenced by the probiotic treatment while still maintaining a stable core dominant community of Enterobacteriacea. The colony with these microbiome had the most improved potential functions in terms of gut microbes as well as the carbohydrates active enzymes most improved potential functions.	0.13%	20.1
Klebsiella michiganensis Species-level Match Host Order Match	RISB1052	Bactrocera dorsalis Order: Diptera	K. michiganensis BD177 has the strain-specific ability to provide three essential amino acids (phenylalanine, tryptophan and methionine) and two vitamins B (folate and riboflavin) to B. dorsalis	0.69%	19.6
Wolbachia pipientis Species-level Match Host Order Match	RISB1515	Drosophila melanogaster Order: Diptera	increases the recombination rate observed across two genomic intervals and increases the efficacy of natural selection in hosts	1.23%	18.8
Citrobacter freundii Species-level Match Host Order Match	RISB1221	Delia antiqua Order: Diptera	six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana through symbiotic bacterium-derived organic acids	0.89%	18.6
Morganella morganii Species-level Match Host Order Match	RISB0772	Delia antiqua Order: Diptera	showed significant volatile inhibition activity against fungal entomopathogen Fusarium moniliforme, Botryosphaeria dothidea and both Fusarium oxysporum respectively	0.24%	18.5
Klebsiella michiganensis Species-level Match Host Order Match	RISB1131	Bactrocera dorsalis Order: Diptera	promotes host resistance to low-temperature stress by stimulating its arginine and proline metabolism pathway in adult Bactrocera dorsalis	0.69%	18.5
Arthrobacter sp. YC-RL1 Species-level Match Host Order Match	RISB0769	Delia antiqua Order: Diptera	showed significant volatile inhibition activity against fungal entomopathogen Fusarium moniliforme, Botryosphaeria dothidea and both Fusarium oxysporum respectively	0.04%	18.3
Paenibacillus sp. BIHB 4019 Species-level Match Host Order Match	RISB0774	Delia antiqua Order: Diptera	showed significant contact inhibition activity against fungal entomopathogen Fusarium moniliforme, Botryosphaeria dothidea and both Fusarium oxysporum respectively	0.04%	18.3
Wolbachia pipientis Species-level Match Host Order Match	RISB1354	Drosophila melanogaster Order: Diptera	Wolbachia influence octopamine metabolism in the Drosophila females, which is by the symbiont genotype	1.23%	18.3
Morganella morganii Species-level Match Host Order Match	RISB0008	Phormia regina Order: Diptera	deterred oviposition by female stable flies; The flies' oviposition decisions appear to be guided by bacteria-derived semiochemicals as the bacteria	0.24%	18.2
Lactococcus lactis Species-level Match Host Order Match	RISB0113	Bactrocera dorsalis Order: Diptera	increase the resistance of B. dorsalis to β-cypermethrin by regulating cytochrome P450 (P450) enzymes and α-glutathione S-transferase (GST) activities	0.13%	18.1
Enterococcus faecalis Species-level Match Host Order Match	RISB1411	Bactrocera dorsalis Order: Diptera	female Bactrocera dorsalis fed Enterococcus faecalis and Klebsiella oxytoca enriched diets lived longer but had lower fecundity	0.12%	17.7
Providencia rettgeri Species-level Match Host Order Match	RISB1001	Anastrepha obliqua Order: Diptera	improve the sexual competitiveness of males	1.78%	17.6
Psychrobacter sp. van23A Species-level Match Host Order Match	RISB1773	Calliphoridae Order: Diptera	it shows physiological adaptation to survival in warmer temperatures and has been previously associated with food spoilage	0.07%	17.5
Psychrobacter sp. WB2 Species-level Match Host Order Match	RISB1773	Calliphoridae Order: Diptera	it shows physiological adaptation to survival in warmer temperatures and has been previously associated with food spoilage	0.06%	17.5
Psychrobacter sp. DAB_AL43B Species-level Match Host Order Match	RISB1773	Calliphoridae Order: Diptera	it shows physiological adaptation to survival in warmer temperatures and has been previously associated with food spoilage	0.02%	17.5
Providencia rettgeri Species-level Match Host Order Match	RISB1169	Bactrocera dorsalis Order: Diptera	Promote the growth of larvae	1.78%	17.3
Citrobacter freundii Species-level Match Host Order Match	RISB1396	Delia antiqua Order: Diptera	suppressed Beauveria bassiana conidia germination and hyphal growth	0.89%	17.2
Morganella morganii Species-level Match Host Order Match	RISB0611	Bactrocera dorsalis Order: Diptera	may hydrolysing nitrogenous waste and providing metabolizable nitrogen for B. dorsalis	0.24%	17.0
Citrobacter freundii Species-level Match Host Order Match	RISB1162	Bactrocera dorsalis Order: Diptera	Promote the growth of larvae	0.89%	16.5
Providencia sp. R33 Species-level Match Host Order Match	RISB1574	Bactrocera tau Order: Diptera	could attract male and female B. tau	0.53%	16.3
Enterococcus faecalis Species-level Match Host Order Match	RISB0095	Bactrocera minax Order: Diptera	egrade phenols in unripe citrus in B. minax larvae	0.12%	16.1
Bacillus thuringiensis Species-level Match Host Order Match	RISB0820	Simulium tani Order: Diptera	show resistance to some antibiotics	0.31%	16.0
Klebsiella pneumoniae Species-level Match Host Order Match	RISB1771	Muscidae Order: Diptera	None	0.98%	16.0
Enterobacter asburiae Species-level Match Host Order Match	RISB1165	Bactrocera dorsalis Order: Diptera	Promote the growth of larvae	0.32%	15.9
Lactococcus lactis Species-level Match Host Order Match	RISB1167	Bactrocera dorsalis Order: Diptera	Promote the growth of larvae	0.13%	15.7
Escherichia coli Species-level Match Host Order Match	RISB1769	Calliphoridae Order: Diptera	None	0.65%	15.7
Bacillus cereus Species-level Match Host Order Match	RISB1872	Aedes aegypti Order: Diptera	gut microbiome	0.35%	15.6
Acinetobacter sp. ESL0695 Species-level Match Host Order Match	RISB2083	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.07%	15.6
Chryseobacterium sp. POL2 Species-level Match Host Order Match	RISB2092	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.05%	15.6
Paenibacillus sp. BIHB 4019 Species-level Match Host Order Match	RISB2098	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.04%	15.6
Enterobacter hormaechei Species-level Match Host Order Match	RISB1331	Zeugodacus cucurbitae Order: Diptera	None	0.59%	15.6
Buchnera aphidicola Species-level Match Host Order Match	RISB0051	Episyrphus balteatus Order: Diptera	None	0.48%	15.5
Bacillus cereus Species-level Match Host Order Match	RISB1701	Phlebotomus papatasi Order: Diptera	None	0.35%	15.4
Staphylococcus hominis Species-level Match Host Order Match	RISB1881	Aedes aegypti Order: Diptera	gut microbiome	0.03%	15.3
Spiroplasma Host Order Match	RISB1796	Drosophila neotestacea Order: Diptera	when parasitized by the nematode Howardula aoronymphium, Spiroplasma encodes a ribosome-inactivating protein (RIP) related to Shiga-like toxins from enterohemorrhagic Escherichia coli and that Howardula ribosomal RNA (rRNA) is depurinated during Spiroplasma-mediated protection of D. neotestacea	0.07%	15.1
Spiroplasma Host Order Match	RISB1926	Anopheles gambiae Order: Diptera	may have reproductive interactions with their mosquito hosts，either providing an indirect fitness advantage to females by inducing male killing or by directly protecting the host against natural pathogens	0.07%	14.2
Spiroplasma Host Order Match	RISB2026	Drosophila hydei Order: Diptera	Spiroplasma protect their host against parasitoid attack. The Spiroplasma-conferred protection is partial and flies surviving a wasp attack have reduced adult longevity and fecundity	0.07%	13.7
Comamonas Host Order Match	RISB2021	Bactrocera dorsalis Order: Diptera	This group in the immature stages may be helping the insects to cope with oxidative stress by supplementing available oxygen.	0.15%	12.7
Raoultella Host Order Match	RISB1575	Bactrocera tau Order: Diptera	could attract male and female B. tau	0.48%	11.2
Aeromonas Host Order Match	RISB2086	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.32%	10.9
Myroides Host Order Match	RISB0626	Musca altica Order: Diptera	None	0.85%	10.9
Buchnera aphidicola Species-level Match	RISB0236	Acyrthosiphon pisum Order: Hemiptera	Buchnera the nutritional endosymbiont of A. pisum is located inside of bacteriocytes and requires aspartate from the aphid host, because it cannot make it de novo. Further Buchnera needs aspartate for the biosynthesis of the essential amino acids lysine and threonine, which the aphid and Buchnera require for survival	0.48%	10.5
Alcaligenes Host Order Match	RISB1871	Aedes aegypti Order: Diptera	gut microbiome	0.16%	10.4
Comamonas Host Order Match	RISB1875	Aedes aegypti Order: Diptera	gut microbiome	0.15%	10.4
Peribacillus Host Order Match	RISB1877	Aedes aegypti Order: Diptera	gut microbiome	0.07%	10.4
Buchnera aphidicola Species-level Match	RISB2485	Macrosiphum euphorbiae Order: Hemiptera	symbiont expression patterns differ between aphid clones with differing levels of virulence, and are influenced by the aphids' host plant. Potentially, symbionts may contribute to differential adaptation of aphids to host plant resistance	0.48%	10.2
Vagococcus Host Order Match	RISB0042	Aldrichina grahami Order: Diptera	None	0.22%	10.2
Comamonas Host Order Match	RISB2020	Bactrocera dorsalis Order: Diptera	None	0.15%	10.2
Microbacterium arborescens Species-level Match	RISB2191	Scirpophaga incertulas Order: Lepidoptera	The ability of these arthropods to feed on wood, foliage and detritus is likely to involve catalysis by different types of cellulases/hemicellulases that are secreted by gut microbiota to digest the structural and recalcitrant lignocellulosic residues in their foods.	0.12%	10.1
Pectobacterium Host Order Match	RISB1772	Muscidae Order: Diptera	None	0.05%	10.1
Apibacter Host Order Match	RISB1138	Musca domestica Order: Diptera	None	0.02%	10.0
Escherichia coli Species-level Match	RISB1339	Manduca sexta Order: Lepidoptera	modulate immunity-related gene expression in the infected F0 larvae, and also in their offspring, triggered immune responses in the infected host associated with shifts in both DNA methylation and histone acetylation	0.65%	10.0
Serratia liquefaciens Species-level Match	RISB1624	Dendroctonus valens Order: Coleoptera	volatiles from predominant bacteria regulate the consumption sequence of carbon sources d-pinitol and d-glucose in the fungal symbiont Leptographium procerum, and appear to alleviate the antagonistic effect from the fungus against RTB larvae	0.10%	9.9
Acinetobacter sp. ESL0695 Species-level Match	RISB0730	Curculio chinensis Order: Coleoptera	Acinetobacter sp. in C. chinensis enriched after treating with saponin, and when incubating bacteria with saponin for 72 h, saponin content significantly decreased from 4.054 to 1.867 mg/mL (by 16S rRNA metagenome sequencing and HPLC)	0.07%	9.8
Clostridium sp. DL-VIII Species-level Match	RISB2301	Pyrrhocoris apterus Order: Hemiptera	could play an important role for the insect by degrading complex dietary components, providing nutrient supplementation, or detoxifying noxious chemicals (e.g. cyclopropenoic fatty acids or gossypol) in the diet	0.05%	9.3
Streptomyces sp. NBC_01324 Species-level Match	RISB0943	Polybia plebeja Order: Hymenoptera	this bacterium produces antimicrobial compounds that are active against Hirsutella citriformis, a natural fungal enemy of its host, and the human pathogens Staphylococcus aureus and Candida albicans	0.26%	9.2
Streptomyces sp. P3 Species-level Match	RISB0943	Polybia plebeja Order: Hymenoptera	this bacterium produces antimicrobial compounds that are active against Hirsutella citriformis, a natural fungal enemy of its host, and the human pathogens Staphylococcus aureus and Candida albicans	0.12%	9.1
Mammaliicoccus sciuri Species-level Match	RISB0075	Bombyx mori Order: Lepidoptera	could produce a secreted chitinolytic lysozyme (termed Msp1) to damage fungal cell walls，completely inhibit the spore germination of fungal entomopathogens Metarhizium robertsii and Beauveria bassiana	0.05%	9.1
Staphylococcus xylosus Species-level Match	RISB2497	Anticarsia gemmatalis Order: Lepidoptera	allow the adaptation of this insect to plants rich in protease inhibitors, minimizing the potentially harmful consequences of protease inhibitors from some of this insect host plants, such as soybean	0.03%	9.0
Streptomyces sp. SJL17-4 Species-level Match	RISB0943	Polybia plebeja Order: Hymenoptera	this bacterium produces antimicrobial compounds that are active against Hirsutella citriformis, a natural fungal enemy of its host, and the human pathogens Staphylococcus aureus and Candida albicans	0.03%	9.0
Acinetobacter pittii Species-level Match	RISB1977	Blattella germanica Order: Blattodea	gut microbiota contributes to production of VCAs that act as fecal aggregation agents and that cockroaches discriminate among the complex odors that emanate from a diverse microbial community	0.09%	8.9
Escherichia coli Species-level Match	RISB0128	Tribolium castaneum Order: Coleoptera	may produce 4,8-dimethyldecanal (DMD) production that is strongly associated with attraction to females and host pheromone communication	0.65%	8.4
Blattabacterium cuenoti Species-level Match	RISB0133	Panesthiinae Order: Blattodea	enables hosts to subsist on a nutrient-poor diet; endosymbiont genome erosions are associated with repeated host transitions to an underground life	0.18%	8.1
Proteus vulgaris Species-level Match	RISB0001	Leptinotarsa decemlineata Order: Coleoptera	produces toxic hydrogen cyanide (HCN) and a mandelonitrile-producing cyanoglucoside, amygdalin, which protect the insect from predation	0.14%	7.8
Enterobacter asburiae Species-level Match	RISB1700	Plutella xylostella Order: Lepidoptera	play an important role in the breakdown of plant cell walls, detoxification of plant phenolics, and synthesis of amino acids.	0.32%	7.8
Enterococcus faecalis Species-level Match	RISB0497	Cryptolestes ferrugineus Order: Coleoptera	bacteria can degrade malathion, pirimiphos-methyl, and deltamethrin and utilize these insecticides as the carbon source in vitro.	0.12%	7.7
Serratia liquefaciens Species-level Match	RISB1801	Dendroctonus valens Order: Coleoptera	could alleviate or compromise the antagonistic effects of fungi O. minus and L. procerum on RTB larval growth	0.10%	7.3
Microbacterium arborescens Species-level Match	RISB1759	Spodoptera frugiperda Order: Lepidoptera	degradation of lambda-cyhalothrin, deltamethrin, chlorpyrifos ethyl, lufenuron and spinosyn	0.12%	6.9
Staphylococcus xylosus Species-level Match	RISB2247	Anticarsia gemmatalis Order: Lepidoptera	mitigation of the negative effects of proteinase inhibitors produced by the host plant	0.03%	6.8
Serratia liquefaciens Species-level Match	RISB2461	Bombyx mori Order: Lepidoptera	able to utilize three polysaccharides including CMcellulose, xylan and pectin	0.10%	6.6
Paenibacillus sp. BIHB 4019 Species-level Match	RISB0813	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-9 oxidation pathway	0.04%	6.4
Microbacterium arborescens Species-level Match	RISB1761	Spodoptera frugiperda Order: Lepidoptera	may influence the metabolization of pesticides in insects	0.12%	6.3
Proteus vulgaris Species-level Match	RISB2460	Bombyx mori Order: Lepidoptera	degradation of cellulose, xylan, pectin and starch	0.14%	6.1
Blattabacterium cuenoti Species-level Match	RISB0518	Cryptocercus punctulatus Order: Blattodea	collaborative arginine biosynthesis	0.18%	5.9
Blattabacterium cuenoti Species-level Match	RISB0093	Blattella germanica Order: Blattodea	obligate endosymbiont	0.18%	5.6
Flavobacterium johnsoniae Species-level Match	RISB0659	Melanaphis bambusae Order: Hemiptera	None	0.11%	5.1
Candidatus Erwinia haradaeae Species-level Match	RISB1632	Lachninae Order: Hemiptera	None	0.04%	5.0
Candidatus Karelsulcia muelleri Species-level Match	RISB1591	Philaenus spumarius Order: Hemiptera	None	0.02%	5.0
Treponema	RISB2377	termite Order: Blattodea	when grown together, two termite-gut Treponema species influence each other's gene expression in a far more comprehensive and nuanced manner than might have been predicted based on the results of previous studies on the respective pure cultures	0.05%	4.9
Apibacter	RISB0603	Apis cerana Order: Hymenoptera	The acquisition of genes for the degradation of the toxic monosaccharides potentiates Apibacter with the ability to utilize the pollen hydrolysis products, at the same time enabling monosaccharide detoxification for the host	0.02%	4.5
Raoultella	RISB2226	Leptinotarsa decemlineata Order: Coleoptera	Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)	0.48%	3.8
Pectobacterium	RISB1889	Pseudococcus longispinus Order: Hemiptera	a nested symbiotic arrangement, where one bacterium lives inside another bacterium,occurred in building the mosaic metabolic pathways seen in mitochondria and plastids	0.05%	3.4
Candidatus Blochmanniella	RISB2542	Camponotus Order: Hymenoptera	Blochmannia provide essential amino acids to its host,Camponotus floridanus, and that it may also play a role in nitrogen recycling via its functional urease	0.02%	3.2
Candidatus Blochmanniella	RISB1827	Camponotus floridanus Order: Hymenoptera	a modulation of immune gene expression which may facilitate tolerance towards the endosymbionts and thus may contribute to their transovarial transmission	0.02%	3.1
Candidatus Blochmanniella	RISB2448	Camponotus floridanus Order: Hymenoptera	nutritional contribution of the bacteria to host metabolism by production of essential amino acids and urease-mediated nitrogen recycling	0.02%	2.8
Streptococcus	RISB2625	Galleria mellonella Order: Lepidoptera	suppress bacteria ingested with food by producing bacteriocin and by releasing a lysozyme like enzyme	0.48%	2.5
Bacteroides	RISB0256	Leptocybe invasa Order: Hymenoptera	Differences in Male-Killing Rickettsia Bacteria between Lineages of the Invasive Gall-Causing Pest Leptocybe invasa	0.09%	2.4
Bacteroides	RISB0090	Hyphantria cunea Order: Lepidoptera	enhance the compatibility of invasive pests to new hosts and enable more rapid adaptation to new habitats.	0.09%	2.2
Bacteroides	RISB1183	Oryzaephilus surinamensis Order: Coleoptera	supplement precursors for the cuticle synthesis and thereby enhance desiccation resistance of its host	0.09%	2.1
Streptococcus	RISB2624	Reticulitermes flavipes Order: Blattodea	can be broken down into substances such as carbon dioxide, ammonia and acetic acid	0.48%	2.1
Raoultella	RISB1672	Spodoptera frugiperda Order: Lepidoptera	downregulated POX but upregulated trypsin PI in this plant species	0.48%	1.8
Bradyrhizobium	RISB0135	Coccinella septempunctata Order: Coleoptera	be commonly found in plant roots and they all have nitrogen fixation abilities	0.16%	1.7
Streptococcus	RISB2604	Homona magnanima Order: Lepidoptera	influence the growth of Bacillus thuringiensis in the larvae	0.48%	1.7
Nostoc	RISB0812	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-18 oxidation pathway	0.10%	1.5
Vibrio	RISB1810	Monochamus galloprovincialis Order: Coleoptera	Have the ability for degradation of cellulose, proteins and starch	0.17%	1.5
Glutamicibacter	RISB0606	Phthorimaea operculella Order: Lepidoptera	could degrade the major toxic α-solanine and α-chaconine in potatoes	0.08%	1.4
Candidatus Mesenet	RISB1785	Brontispa longissima Order: Coleoptera	induced complete Cytoplasmic incompatibility (CI) (100% mortality)	0.02%	1.3
Aeromonas	RISB2456	Bombyx mori Order: Lepidoptera	able to utilize the CMcellulose and xylan	0.32%	1.1
Paraclostridium	RISB0028	Sesamia inferens Order: Lepidoptera	degrade Chlorpyrifos and Chlorantraniliprole in vitro	0.08%	1.1
Pectobacterium	RISB0798	Pseudoregma bambucicola Order: Hemiptera	may help P. bambucicola feed on the stalks of bamboo	0.05%	1.1
Lysinibacillus	RISB1416	Psammotermes hypostoma Order: Blattodea	isolates showed significant cellulolytic activity	0.08%	1.1
Curtobacterium	RISB1910	Hyles euphorbiae Order: Lepidoptera	able to degrade alkaloids and/or latex	0.18%	0.9
Aeromonas	RISB1145	Tenebrio molitor Order: Coleoptera	degrading plastics	0.32%	0.7
Priestia	RISB0839	Helicoverpa armigera Order: Lepidoptera	producing amylase	0.18%	0.5
Lysinibacillus	RISB1066	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.08%	0.3
Curtobacterium	RISB0900	Myzus persicae Order: Hemiptera	None	0.18%	0.2
Weeksella	RISB1265	Rheumatobates bergrothi Order: Hemiptera	None	0.09%	0.1
Glutamicibacter	RISB0438	Helicoverpa armigera Order: Lepidoptera	None	0.08%	0.1
Treponema	RISB0169	Reticulitermes flaviceps Order: Blattodea	None	0.05%	0.1
Legionella	RISB1687	Polyplax serrata Order: Phthiraptera	None	0.05%	0.1
Helicobacter	RISB0662	Melanaphis bambusae Order: Hemiptera	None	0.04%	0.0
Apibacter	RISB0604	Apis cerana Order: Hymenoptera	None	0.02%	0.0