SRR5940752 - Musca domestica

Basic Information

Run: SRR5940752

Assay Type: WGS

Bioproject: PRJNA385554

Biosample: SAMN07135705

Bytes: 8302015063

Center Name: NANYANG TECHNOLOGICAL UNIVERSITY

Sequencing Information

Instrument: Illumina HiSeq 2500

Library Layout: PAIRED

Library Selection: RANDOM

Platform: ILLUMINA

Geographic Information

Country: Singapore

Continent: Asia

Location Name: Singapore

Latitude/Longitude: 1.344255 N 103.679553 E

Sample Information

Host: Musca domestica

Isolation: NTU campus

Biosample Model: Metagenome or environmental

Collection Date: 2014-05-23

Taxonomic Classification

Potential Symbionts

About Potential Symbionts

This table shows potential symbiont identified in the metagenome sample. Matches are scored based on:

Relative abundance in the sample
Species-level matches with known symbionts
Host insect order matches with reference records
Completeness and richness of functional records

Note: only the three highest scoring comparison records were retained for each symbiont species or genus in the sample

Based on our current records database, this section aims to identify potential functional symbionts in this metagenome sample, with scoring based on:

Relative abundance in sample
Species-level matches with known symbionts
Host insect order matches
Functional record completeness

Note: Showing top 3 highest scoring records for each species/genus

Symbiont Name	Record	Host Species	Function	Abundance	Score Score Composition: Symbiont Abundance Species match bonus Host match bonus Function richness Higher scores indicate stronger symbiotic relationship potential
Klebsiella oxytoca Species-level Match Host Order Match Host Species Match	RISB1139	Musca domestica Order: Diptera	It is associated to newly laid housefly eggs, where it is deposited by the female, and has a role in oviposition as well as protection against potential pathogens	0.01%	38.3
Apibacter Host Order Match Host Species Match	RISB1138	Musca domestica Order: Diptera	None	0.35%	30.4
Wolbachia Host Order Match	RISB0766	Aedes fluviatilis Order: Diptera	The presence of Wolbachia pipientis improves energy performance in A. fluviatilis cells; it affects the regulation of key energy sources such as lipids, proteins, and carbohydrates, making the distribution of actin more peripheral and with extensions that come into contact with neighboring cells.	6.05%	21.1
Wolbachia Host Order Match	RISB0779	Drosophila melanogaster Order: Diptera	Wolbachia infection affects differential gene expression in Drosophila testis.Genes involved in carbohydrate metabolism, lysosomal degradation, proteolysis, lipid metabolism, and immune response were upregulated in the presence of Wolbachia	6.05%	20.9
Wolbachia Host Order Match	RISB1408	Anastrepha fraterculus Order: Diptera	Wolbachia is the only known reproductive symbiont present in these morphotypes. Wolbachia reduced the ability for embryonic development in crosses involving cured females and infected males within each morphotype (uni-directional CI).	6.05%	20.7
Lactococcus lactis Species-level Match Host Order Match	RISB0131	Ceratitis capitata Order: Diptera	The intestinal microbiota structure was significantly influenced by the probiotic treatment while still maintaining a stable core dominant community of Enterobacteriacea. The colony with these microbiome had the most improved potential functions in terms of gut microbes as well as the carbohydrates active enzymes most improved potential functions.	0.25%	20.3
Enterobacter sp. Colony194 Species-level Match Host Order Match	RISB0893	Bactrocera dorsalis Order: Diptera	be beneficial, with some quality control indices, such as adult size, pupal weight, survival rate under stress and nutritionally rich conditions, and mating competitiveness, being significantly increased, while slight nonsignificant increases in emergence rate and flight ability were observed	0.08%	20.1
Listeria monocytogenes Species-level Match Host Order Match	RISB2308	Drosophila melanogaster Order: Diptera	L. monocytogenes infection disrupts host energy metabolism by depleting energy stores (triglycerides and glycogen) and reducing metabolic pathway activity (beta-oxidation and glycolysis). The infection affects antioxidant defense by reducing uric acid levels and alters amino acid metabolism. These metabolic changes are accompanied by melanization, potentially linked to decreased tyrosine levels.	0.06%	20.1
Klebsiella oxytoca Species-level Match Host Order Match	RISB0130	Ceratitis capitata Order: Diptera	The intestinal microbiota structure was significantly influenced by the probiotic treatment while still maintaining a stable core dominant community of Enterobacteriacea. The colony with these microbiome had the most improved potential functions in terms of gut microbes as well as the carbohydrates active enzymes most improved potential functions.	0.01%	20.0
Enterobacter sp. Colony194 Species-level Match Host Order Match	RISB1338	Ceratitis capitata Order: Diptera	Enterobacter sp. AA26 dry biomass can fully replace the brewer’s yeast as a protein source in medfly larval diet without any effect on the productivity and the biological quality of reared medfly of VIENNA 8 GSS	0.08%	19.3
Klebsiella michiganensis Species-level Match Host Order Match	RISB1052	Bactrocera dorsalis Order: Diptera	K. michiganensis BD177 has the strain-specific ability to provide three essential amino acids (phenylalanine, tryptophan and methionine) and two vitamins B (folate and riboflavin) to B. dorsalis	0.01%	18.9
Serratia marcescens Species-level Match Host Order Match	RISB1291	Aedes aegypti Order: Diptera	facilitates arboviral infection through a secreted protein named SmEnhancin, which digests membrane-bound mucins on the mosquito gut epithelia, thereby enhancing viral dissemination.	0.03%	18.7
Morganella morganii Species-level Match Host Order Match	RISB0772	Delia antiqua Order: Diptera	showed significant volatile inhibition activity against fungal entomopathogen Fusarium moniliforme, Botryosphaeria dothidea and both Fusarium oxysporum respectively	0.03%	18.3
Paenibacillus sp. FSL W8-0194 Species-level Match Host Order Match	RISB0774	Delia antiqua Order: Diptera	showed significant contact inhibition activity against fungal entomopathogen Fusarium moniliforme, Botryosphaeria dothidea and both Fusarium oxysporum respectively	0.05%	18.3
Acinetobacter guillouiae Species-level Match Host Order Match	RISB0768	Delia antiqua Order: Diptera	showed significant volatile inhibition activity against fungal entomopathogen Fusarium moniliforme, Botryosphaeria dothidea and both Fusarium oxysporum respectively	0.02%	18.3
Arthrobacter sp. NicSoilB8 Species-level Match Host Order Match	RISB0769	Delia antiqua Order: Diptera	showed significant volatile inhibition activity against fungal entomopathogen Fusarium moniliforme, Botryosphaeria dothidea and both Fusarium oxysporum respectively	0.02%	18.3
Paenibacillus sp. IHBB 10380 Species-level Match Host Order Match	RISB0774	Delia antiqua Order: Diptera	showed significant contact inhibition activity against fungal entomopathogen Fusarium moniliforme, Botryosphaeria dothidea and both Fusarium oxysporum respectively	0.01%	18.3
Lactococcus lactis Species-level Match Host Order Match	RISB0113	Bactrocera dorsalis Order: Diptera	increase the resistance of B. dorsalis to β-cypermethrin by regulating cytochrome P450 (P450) enzymes and α-glutathione S-transferase (GST) activities	0.25%	18.3
Wigglesworthia glossinidia Species-level Match Host Order Match	RISB0369	Glossina morsitans Order: Diptera	symbiont-derived factors, likely B vitamins, are critical for the proper function of both lipid biosynthesis and lipolysis to maintain tsetse fly fecundity	0.02%	18.1
Enterococcus casseliflavus Species-level Match Host Order Match	RISB0112	Bactrocera dorsalis Order: Diptera	increase the resistance of B. dorsalis to β-cypermethrin by regulating cytochrome P450 (P450) enzymes and α-glutathione S-transferase (GST) activities	0.02%	18.0
Morganella morganii Species-level Match Host Order Match	RISB0008	Phormia regina Order: Diptera	deterred oviposition by female stable flies; The flies' oviposition decisions appear to be guided by bacteria-derived semiochemicals as the bacteria	0.03%	18.0
Spiroplasma poulsonii Species-level Match Host Order Match	RISB1346	Drosophila melanogaster Order: Diptera	S. poulsonii protects its host against parasitoid wasps and nematodes by the action of toxins from the family of Ribosome Inactivating Proteins	0.03%	17.9
Stenotrophomonas maltophilia Species-level Match Host Order Match	RISB1227	Delia antiqua Order: Diptera	six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana through symbiotic bacterium-derived organic acids	0.12%	17.8
Enterococcus faecalis Species-level Match Host Order Match	RISB1411	Bactrocera dorsalis Order: Diptera	female Bactrocera dorsalis fed Enterococcus faecalis and Klebsiella oxytoca enriched diets lived longer but had lower fecundity	0.23%	17.8
Citrobacter freundii Species-level Match Host Order Match	RISB1221	Delia antiqua Order: Diptera	six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana through symbiotic bacterium-derived organic acids	0.05%	17.8
Sphingobacterium faecium Species-level Match Host Order Match	RISB1226	Delia antiqua Order: Diptera	six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana through symbiotic bacterium-derived organic acids	0.02%	17.7
Serratia marcescens Species-level Match Host Order Match	RISB0009	Phormia regina Order: Diptera	prompted oviposition by flies; The flies' oviposition decisions appear to be guided by bacteria-derived semiochemicals as the bacteria	0.03%	17.7
Exiguobacterium sp. Helios Species-level Match Host Order Match	RISB0007	Phormia regina Order: Diptera	prompted oviposition by flies; The flies' oviposition decisions appear to be guided by bacteria-derived semiochemicals as the bacteria	0.01%	17.7
Spiroplasma poulsonii Species-level Match Host Order Match	RISB2264	Drosophila melanogaster Order: Diptera	Spiroplasma coopts the yolk transport and uptake machinery to colonize the germ line and ensure efficient vertical transmission	0.03%	17.6
Spiroplasma poulsonii Species-level Match Host Order Match	RISB1928	Drosophila melanogaster Order: Diptera	supporting the hypothesis that competition for host lipids underlies S. poulsonii-mediated protection against parasitoid wasps	0.03%	17.6
Psychrobacter sp. KFRI-CH2-11 Species-level Match Host Order Match	RISB1773	Calliphoridae Order: Diptera	it shows physiological adaptation to survival in warmer temperatures and has been previously associated with food spoilage	0.01%	17.5
Bacillus thuringiensis Species-level Match Host Order Match	RISB0820	Simulium tani Order: Diptera	show resistance to some antibiotics	1.50%	17.2
Wigglesworthia glossinidia Species-level Match Host Order Match	RISB1786	Glossina morsitans Order: Diptera	Synthesis of a large number of B vitamins, to supplement the host nutritional deficiencies of the diet	0.02%	17.1
Stenotrophomonas maltophilia Species-level Match Host Order Match	RISB1141	Hermetia illucens Order: Diptera	enhance the insect growth performance when reared on an unbalanced nutritionally poor diet	0.12%	16.9
Enterobacter sp. Colony194 Species-level Match Host Order Match	RISB1311	Ceratitis capitata Order: Diptera	it was shown to have positive effects in rearing efficiency when used as larval probiotics	0.08%	16.9
Morganella morganii Species-level Match Host Order Match	RISB0611	Bactrocera dorsalis Order: Diptera	may hydrolysing nitrogenous waste and providing metabolizable nitrogen for B. dorsalis	0.03%	16.8
Bacillus sp. Y1 Species-level Match Host Order Match	RISB0791	Anopheles barbirostris Order: Diptera	without this midgut flora showed delayed development to become adult	0.18%	16.5
Pantoea dispersa Species-level Match Host Order Match	RISB1413	Bactrocera dorsalis Order: Diptera	causing female Bactrocera dorsalis laid more eggs but had shorter lifespan	0.03%	16.5
Stenotrophomonas maltophilia Species-level Match Host Order Match	RISB1401	Delia antiqua Order: Diptera	suppressed Beauveria bassiana conidia germination and hyphal growth	0.12%	16.5
Bacillus sp. LJBS17 Species-level Match Host Order Match	RISB0791	Anopheles barbirostris Order: Diptera	without this midgut flora showed delayed development to become adult	0.06%	16.4
Citrobacter freundii Species-level Match Host Order Match	RISB1396	Delia antiqua Order: Diptera	suppressed Beauveria bassiana conidia germination and hyphal growth	0.05%	16.4
Sphingobacterium faecium Species-level Match Host Order Match	RISB1400	Delia antiqua Order: Diptera	suppressed Beauveria bassiana conidia germination and hyphal growth	0.02%	16.4
Enterococcus faecalis Species-level Match Host Order Match	RISB0095	Bactrocera minax Order: Diptera	egrade phenols in unripe citrus in B. minax larvae	0.23%	16.2
Wigglesworthia glossinidia Species-level Match Host Order Match	RISB2577	Glossina brevipalpis Order: Diptera	provide its tsetse host with metabolites such as vitamins	0.02%	16.2
Lactiplantibacillus plantarum Species-level Match Host Order Match	RISB0674	Drosophila melanogaster Order: Diptera	could effectively inhibit fungal spore germinations	0.04%	16.1
Serratia marcescens Species-level Match Host Order Match	RISB0096	Bactrocera minax Order: Diptera	egrade phenols in unripe citrus in B. minax larvae	0.03%	16.0
Providencia rettgeri Species-level Match Host Order Match	RISB1001	Anastrepha obliqua Order: Diptera	improve the sexual competitiveness of males	0.11%	16.0
Escherichia coli Species-level Match Host Order Match	RISB1769	Calliphoridae Order: Diptera	None	0.86%	15.9
Lactococcus lactis Species-level Match Host Order Match	RISB1167	Bactrocera dorsalis Order: Diptera	Promote the growth of larvae	0.25%	15.8
Buchnera aphidicola Species-level Match Host Order Match	RISB0051	Episyrphus balteatus Order: Diptera	None	0.79%	15.8
Acinetobacter sp. Colony158 Species-level Match Host Order Match	RISB2083	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.11%	15.7
Providencia rettgeri Species-level Match Host Order Match	RISB1169	Bactrocera dorsalis Order: Diptera	Promote the growth of larvae	0.11%	15.7
Acinetobacter sp. GSS19 Species-level Match Host Order Match	RISB2083	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.09%	15.7
Chryseobacterium sp. StRB126 Species-level Match Host Order Match	RISB2092	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.07%	15.6
Paenibacillus sp. FSL W8-0194 Species-level Match Host Order Match	RISB2098	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.05%	15.6
Citrobacter freundii Species-level Match Host Order Match	RISB1162	Bactrocera dorsalis Order: Diptera	Promote the growth of larvae	0.05%	15.6
Chryseobacterium sp. POL2 Species-level Match Host Order Match	RISB2092	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.05%	15.6
Chryseobacterium sp. Y16C Species-level Match Host Order Match	RISB2092	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.04%	15.6
Providencia alcalifaciens Species-level Match Host Order Match	RISB1168	Bactrocera dorsalis Order: Diptera	Promote the growth of larvae	0.03%	15.6
Staphylococcus hominis Species-level Match Host Order Match	RISB1881	Aedes aegypti Order: Diptera	gut microbiome	0.04%	15.3
Candidatus Cardinium Host Order Match	RISB1439	Lutzomyia evansi Order: Diptera	‘Candidatus Cardinium’ is a recently described bacterium from the Bacteroidetes group involved in diverse reproduction alterations of its arthropod hosts (including cytoplasmic incompatibility, parthenogenesis, and feminization) similar to Wolbachia	0.23%	15.2
Lactiplantibacillus plantarum Species-level Match Host Order Match	RISB0608	Drosophila melanogaster Order: Diptera	None	0.04%	15.0
Sodalis Host Order Match	RISB2256	Glossina palpalis Order: Diptera	flies harbouring this symbiont have three times greater probability of being infected by trypanosomes than flies without the symbiont.	0.02%	12.7
Shewanella Host Order Match	RISB1924	Anopheles gambiae Order: Diptera	may be related with mediating adaptation to different ecological niches or in shaping specific adult behaviors including mating	0.12%	12.7
Streptomyces sp. NBC_01324 Species-level Match	RISB0943	Polybia plebeja Order: Hymenoptera	this bacterium produces antimicrobial compounds that are active against Hirsutella citriformis, a natural fungal enemy of its host, and the human pathogens Staphylococcus aureus and Candida albicans	2.79%	11.8
Streptomyces sp. T12 Species-level Match	RISB0943	Polybia plebeja Order: Hymenoptera	this bacterium produces antimicrobial compounds that are active against Hirsutella citriformis, a natural fungal enemy of its host, and the human pathogens Staphylococcus aureus and Candida albicans	2.66%	11.6
Sodalis Host Order Match	RISB2471	Glossina morsitans Order: Diptera	retains a thiamine ABC transporter (tbpAthiPQ) believed to salvage thiamine	0.02%	11.5
Streptomyces sp. NBC_01324 Species-level Match	RISB2334	Sirex noctilio Order: Hymenoptera	degrading woody substrates and that such degradation may assist in nutrient acquisition by S. noctilio, thus contributing to its ability to be established in forested habitats worldwide	2.79%	11.5
Sodalis Host Order Match	RISB2531	Glossina spp. Order: Diptera	quorum sensing primes the oxidative stress response of endosymbiont	0.02%	11.4
Dysgonomonas Host Order Match	RISB1235	Hermetia illucens Order: Diptera	provides the tools for degrading of a broad range of substrates	0.03%	11.3
Photorhabdus Host Order Match	RISB0532	Drosophila melanogaster Order: Diptera	produces toxin complex (Tc) toxins as major virulence factors	0.05%	11.3
Buchnera aphidicola Species-level Match	RISB0236	Acyrthosiphon pisum Order: Hemiptera	Buchnera the nutritional endosymbiont of A. pisum is located inside of bacteriocytes and requires aspartate from the aphid host, because it cannot make it de novo. Further Buchnera needs aspartate for the biosynthesis of the essential amino acids lysine and threonine, which the aphid and Buchnera require for survival	0.79%	10.8
Cedecea Host Order Match	RISB1570	Bactrocera tau Order: Diptera	could attract male and female B. tau	0.02%	10.7
Aeromonas Host Order Match	RISB2086	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.07%	10.6
Aquitalea Host Order Match	RISB2089	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.01%	10.6
Buchnera aphidicola Species-level Match	RISB2485	Macrosiphum euphorbiae Order: Hemiptera	symbiont expression patterns differ between aphid clones with differing levels of virulence, and are influenced by the aphids' host plant. Potentially, symbionts may contribute to differential adaptation of aphids to host plant resistance	0.79%	10.6
Peribacillus Host Order Match	RISB1877	Aedes aegypti Order: Diptera	gut microbiome	0.22%	10.5
Pseudomonas sp. CIP-10 Species-level Match	RISB1622	Dendroctonus valens Order: Coleoptera	volatiles from predominant bacteria regulate the consumption sequence of carbon sources d-pinitol and d-glucose in the fungal symbiont Leptographium procerum, and appear to alleviate the antagonistic effect from the fungus against RTB larvae	0.54%	10.4
Sphingobium Host Order Match	RISB1880	Aedes aegypti Order: Diptera	gut microbiome	0.02%	10.3
Vagococcus Host Order Match	RISB0042	Aldrichina grahami Order: Diptera	None	0.19%	10.2
Escherichia coli Species-level Match	RISB1339	Manduca sexta Order: Lepidoptera	modulate immunity-related gene expression in the infected F0 larvae, and also in their offspring, triggered immune responses in the infected host associated with shifts in both DNA methylation and histone acetylation	0.86%	10.2
Myroides Host Order Match	RISB0626	Musca altica Order: Diptera	None	0.18%	10.2
Pectobacterium Host Order Match	RISB1772	Muscidae Order: Diptera	None	0.05%	10.1
Francisella tularensis Species-level Match	RISB1907	Bombyx mori Order: Lepidoptera	After infection with F. tularensis, the induction of melanization and nodulation, which are immune responses to bacterial infection, were inhibited in silkworms. Pre-inoculation of silkworms with F. tularensis enhanced the expression of antimicrobial peptides and resistance to infection by pathogenic bacteria.	0.03%	10.0
Chroococcidiopsis Host Order Match	RISB0487	Ceratitis capitata Order: Diptera	None	0.03%	10.0
Pantoea agglomerans Species-level Match	RISB2197	Termitidae Order: Blattodea	The ability of these arthropods to feed on wood, foliage and detritus is likely to involve catalysis by different types of cellulases/hemicellulases that are secreted by gut microbiota to digest the structural and recalcitrant lignocellulosic residues in their foods.	0.02%	10.0
Gilliamella apicola Species-level Match	RISB0102	Apis mellifera Order: Hymenoptera	Gilliamella apicola carries the gene for the desaturase FADS2, which is able to metabolize polyunsaturated fatty acids from pollen and synthesize endocannabinoid, a lipogenic neuroactive substance, thereby modulating reward learning and memory in honeybees.	0.02%	10.0
Variovorax Host Order Match	RISB1712	Phlebotomus papatasi Order: Diptera	None	0.02%	10.0
Candidatus Pantoea carbekii Species-level Match	RISB1046	Halyomorpha halys Order: Hemiptera	provides its host with essential nutrients, vitamins, cofactors and protection of the most vulnerable stages of early development (1st nymphal stages). Pantoea carbekii is highly stress tolerant, especially once secreted to cover the eggs, by its unique biofilm-formation properties, securing host offspring survival	0.01%	10.0
Staphylococcus gallinarum Species-level Match	RISB0945	Callosobruchus maculatus Order: Coleoptera	The strain encodes complete biosynthetic pathways for the production of B vitamins and amino acids, including tyrosine; A carbohydrate-active enzyme search revealed that the genome codes for a number of digestive enzymes, reflecting the nutritional ecology of C. maculatus	0.01%	10.0
Pseudomonas sp. StFLB209 Species-level Match	RISB1622	Dendroctonus valens Order: Coleoptera	volatiles from predominant bacteria regulate the consumption sequence of carbon sources d-pinitol and d-glucose in the fungal symbiont Leptographium procerum, and appear to alleviate the antagonistic effect from the fungus against RTB larvae	0.01%	9.8
Candidatus Nasuia deltocephalinicola Species-level Match	RISB2283	Nephotettix cincticeps Order: Hemiptera	Oral administration of tetracycline to nymphal N. cincticeps resulted in retarded growth, high mortality rates, and failure in adult emergence, suggesting important biological roles of the symbionts for the host insect	0.01%	9.4
Clostridium sp. LQ25 Species-level Match	RISB2301	Pyrrhocoris apterus Order: Hemiptera	could play an important role for the insect by degrading complex dietary components, providing nutrient supplementation, or detoxifying noxious chemicals (e.g. cyclopropenoic fatty acids or gossypol) in the diet	0.12%	9.3
Clostridium sp. DL-VIII Species-level Match	RISB2301	Pyrrhocoris apterus Order: Hemiptera	could play an important role for the insect by degrading complex dietary components, providing nutrient supplementation, or detoxifying noxious chemicals (e.g. cyclopropenoic fatty acids or gossypol) in the diet	0.06%	9.3
Clostridium sp. JS66 Species-level Match	RISB2301	Pyrrhocoris apterus Order: Hemiptera	could play an important role for the insect by degrading complex dietary components, providing nutrient supplementation, or detoxifying noxious chemicals (e.g. cyclopropenoic fatty acids or gossypol) in the diet	0.05%	9.3
Candidatus Carsonella ruddii Species-level Match	RISB0394	Cacopsylla pyricola Order: Hemiptera	Carsonella produces most essential amino acids (EAAs) for C. pyricola, Psyllophila complements the genes missing in Carsonella for the tryptophan pathway and synthesizes some vitamins and carotenoids	0.10%	9.1
Mammaliicoccus sciuri Species-level Match	RISB0075	Bombyx mori Order: Lepidoptera	could produce a secreted chitinolytic lysozyme (termed Msp1) to damage fungal cell walls，completely inhibit the spore germination of fungal entomopathogens Metarhizium robertsii and Beauveria bassiana	0.07%	9.1
Candidatus Schneideria nysicola Species-level Match	RISB0872	Nysius sp. Order: Hemiptera	synthesize four B vitamins(Pan, pantothenate;Fol, folate; Rib, riboflavin; Pyr, pyridoxine) and five Essential Amino Acids(Ile, isoleucine; Val, valine; Lys, lysine; Thr, threonine; Phe, phenylalanine)	0.01%	9.0
Staphylococcus xylosus Species-level Match	RISB2497	Anticarsia gemmatalis Order: Lepidoptera	allow the adaptation of this insect to plants rich in protease inhibitors, minimizing the potentially harmful consequences of protease inhibitors from some of this insect host plants, such as soybean	0.03%	9.0
Pseudomonas sp. CIP-10 Species-level Match	RISB2224	Leptinotarsa decemlineata Order: Coleoptera	Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)	0.54%	8.9
Escherichia coli Species-level Match	RISB0128	Tribolium castaneum Order: Coleoptera	may produce 4,8-dimethyldecanal (DMD) production that is strongly associated with attraction to females and host pheromone communication	0.86%	8.6
Candidatus Portiera aleyrodidarum Species-level Match	RISB1193	Bemisia tabaci Order: Hemiptera	synthesizing essential amino acid (e.g. tryptophan, leucine and L-Isoleucine), Bemisia tabaci provides vital nutritional support for growth, development and reproduction	0.01%	8.4
Lactobacillus sp. PV012 Species-level Match	RISB0292	Lymantria dispar asiatica Order: Lepidoptera	Beauveria bassiana infection-based assays showed that the mortality of non-axenic L. dispar asiatica larvae was significantly higher than that of axenic larvae at 72 h.	0.03%	8.4
Lactobacillus sp. IBH004 Species-level Match	RISB0292	Lymantria dispar asiatica Order: Lepidoptera	Beauveria bassiana infection-based assays showed that the mortality of non-axenic L. dispar asiatica larvae was significantly higher than that of axenic larvae at 72 h.	0.01%	8.4
Sphingobacterium sp. ML3W Species-level Match	RISB2227	Leptinotarsa decemlineata Order: Coleoptera	Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)	0.02%	8.4
Candidatus Gullanella endobia Species-level Match	RISB1885	Ferrisia virgata Order: Hemiptera	a nested symbiotic arrangement, where one bacterium lives inside another bacterium,occurred in building the mosaic metabolic pathways seen in mitochondria and plastids	0.01%	8.4
Blattabacterium cuenoti Species-level Match	RISB0133	Panesthiinae Order: Blattodea	enables hosts to subsist on a nutrient-poor diet; endosymbiont genome erosions are associated with repeated host transitions to an underground life	0.40%	8.3
Proteus vulgaris Species-level Match	RISB0001	Leptinotarsa decemlineata Order: Coleoptera	produces toxic hydrogen cyanide (HCN) and a mandelonitrile-producing cyanoglucoside, amygdalin, which protect the insect from predation	0.04%	7.7
Carnobacterium maltaromaticum Species-level Match	RISB1693	Plutella xylostella Order: Lepidoptera	play an important role in the breakdown of plant cell walls, detoxification of plant phenolics, and synthesis of amino acids.	0.02%	7.5
Candidatus Nasuia deltocephalinicola Species-level Match	RISB2282	Nephotettix cincticeps Order: Hemiptera	With the antibiotic, nymphal growth was remarkably retarded, and a number of nymphs either died or failed to attain adulthood	0.01%	7.5
Candidatus Tachikawaea gelatinosa Species-level Match	RISB2112	Urostylis westwoodii Order: Hemiptera	the symbiont localizes to a specialized midgut region and supplies essential amino acids deficient in the host's diet	0.01%	7.4
Candidatus Nasuia deltocephalinicola Species-level Match	RISB0262	Maiestas dorsalis Order: Hemiptera	are responsible for synthesizing two essential amino acids (histidine and methionine) and riboflavin (vitamin B2)	0.01%	7.3
Candidatus Portiera aleyrodidarum Species-level Match	RISB2289	Bemisia tabaci Order: Hemiptera	encoding the capability to synthetize, or participate in the synthesis of, several amino acids and carotenoids,	0.01%	7.2
Candidatus Rickettsiella viridis Species-level Match	RISB1949	Acyrthosiphon pisum Order: Hemiptera	young red aphid larvae infected whith symbiont become greener at adulthood,which can reduce predation risk	0.01%	7.1
Apilactobacillus kunkeei Species-level Match	RISB0475	Apis mellifera Order: Hymenoptera	A. kunkeei alleviated acetamiprid-induced symbiotic microbiota dysregulation and mortality in honeybees	0.01%	7.1
Candidatus Portiera aleyrodidarum Species-level Match	RISB1973	Bemisia tabaci Order: Hemiptera	a primary symbiont, which compensates for the deficient nutritional composition of its food sources	0.01%	7.0
Snodgrassella alvi Species-level Match	RISB1423	Bombus spp. Order: Hymenoptera	The bumble bee microbiome slightly increases survivorship when the host is exposed to selenate	0.01%	6.9
Paludibacter propionicigenes Species-level Match	RISB2055	Odontotaenius disjunctus Order: Coleoptera	microbial fixation of nitrogen that is important for this beetle to subsist on woody biomass	0.01%	6.9
Leclercia adecarboxylata Species-level Match	RISB1757	Spodoptera frugiperda Order: Lepidoptera	degradation of lambda-cyhalothrin, deltamethrin, chlorpyrifos ethyl, lufenuron and spinosyn	0.01%	6.8
Blattabacterium sp. (Cryptocercus kyebangensis) Species-level Match	RISB1534	Periplaneta fuliginosa Order: Blattodea	involved in uric acid degradation, nitrogen assimilation and nutrient provisioning	0.01%	6.7
Carnobacterium maltaromaticum Species-level Match	RISB1692	Plutella xylostella Order: Lepidoptera	participate in the synthesis of host lacking amino acids histidine and threonine	0.02%	6.6
Frischella perrara Species-level Match	RISB2028	Diceroprocta semicincta Order: Hemiptera	causes the formation of a scab-like structure on the gut epithelium of its host	0.01%	6.6
Candidatus Rickettsiella viridis Species-level Match	RISB0277	Myzus persicae Order: Hemiptera	parasitoids showing a preference for probing aphids infected with R. viridis	0.01%	6.5
Xenorhabdus bovienii Species-level Match	RISB2270	Acyrthosiphon pisum Order: Hemiptera	have the gene PIN1 encoding the protease inhibitor protein against aphids	0.01%	6.5
Lysinibacillus fusiformis Species-level Match	RISB1417	Psammotermes hypostoma Order: Blattodea	isolates showed significant cellulolytic activity	0.18%	6.2
Leclercia adecarboxylata Species-level Match	RISB1758	Spodoptera frugiperda Order: Lepidoptera	may influence the metabolization of pesticides in insects	0.01%	6.2
Blattabacterium cuenoti Species-level Match	RISB0518	Cryptocercus punctulatus Order: Blattodea	collaborative arginine biosynthesis	0.40%	6.1
Proteus vulgaris Species-level Match	RISB2460	Bombyx mori Order: Lepidoptera	degradation of cellulose, xylan, pectin and starch	0.04%	6.0
Paludibacter propionicigenes Species-level Match	RISB2056	Odontotaenius disjunctus Order: Coleoptera	plays an important role in nitrogen fixation	0.01%	5.9
Carnobacterium maltaromaticum Species-level Match	RISB1691	Plutella xylostella Order: Lepidoptera	activity of cellulose and hemicellulose	0.02%	5.8
Salmonella enterica Species-level Match	RISB0413	Melanaphis sacchari Order: Hemiptera	None	0.49%	5.5
Lysinibacillus fusiformis Species-level Match	RISB1066	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.18%	5.4
Treponema	RISB2377	termite Order: Blattodea	when grown together, two termite-gut Treponema species influence each other's gene expression in a far more comprehensive and nuanced manner than might have been predicted based on the results of previous studies on the respective pure cultures	0.51%	5.4
Exiguobacterium sp. Helios Species-level Match	RISB1152	Tenebrio molitor Order: Coleoptera	degrading plastics	0.01%	5.4
Arsenophonus nasoniae Species-level Match	RISB0428	Nasonia vitripennis Order: Hymenoptera	male killing	0.04%	5.3
Diaphorobacter aerolatus Species-level Match	RISB1062	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.02%	5.2
Candidatus Cardinium	RISB0223	Bemisia tabaci Order: Hemiptera	Cardinium could inhibit the defense response of the host plant and decrease the detoxification metabolism ability of the host whitefly, decrease the expression of detoxification metabolism genes, especially the uridine 5'-diphospho-glucuronyltransferase and P450 genes,	0.23%	5.2
Vibrio	RISB1810	Monochamus galloprovincialis Order: Coleoptera	Have the ability for degradation of cellulose, proteins and starch	3.86%	5.2
Flavobacterium johnsoniae Species-level Match	RISB0659	Melanaphis bambusae Order: Hemiptera	None	0.14%	5.1
Candidatus Carsonella ruddii Species-level Match	RISB0748	Diaphorina citri Order: Hemiptera	None	0.10%	5.1
Candidatus Erwinia haradaeae Species-level Match	RISB1632	Lachninae Order: Hemiptera	None	0.09%	5.1
Burkholderia	RISB1172	Lagria villosa Order: Coleoptera	process a cryptic gene cluster that codes for the biosynthesis of a novel antifungal polyketide with a glutarimide pharmacophore, which led to the discovery of the gladiofungins as previously-overlooked components of the antimicrobial armory of the beetle symbiont	0.09%	5.1
Candidatus Megaera polyxenophila Species-level Match	RISB0587	Multiple species Order: None	None	0.07%	5.1
Arsenophonus nasoniae Species-level Match	RISB0366	Pachycrepoideus vindemmiae Order: Hymenoptera	None	0.04%	5.0
Agrobacterium tumefaciens Species-level Match	RISB0650	Melanaphis bambusae Order: Hemiptera	None	0.04%	5.0
Bifidobacterium	RISB0174	Apis mellifera Order: Hymenoptera	Bifidobacterium provides complementary demethylation service to promote Gilliamella growth on methylated homogalacturonan, an enriched polysaccharide of pectin. In exchange, Gilliamella shares digestive products with Bifidobacterium, through which a positive interaction is established	0.04%	5.0
Candidatus Palibaumannia cicadellinicola Species-level Match	RISB1594	Graphocephala coccinea Order: Hemiptera	None	0.03%	5.0
Candidatus Karelsulcia muelleri Species-level Match	RISB1591	Philaenus spumarius Order: Hemiptera	None	0.03%	5.0
Gilliamella apicola Species-level Match	RISB1945	Apis cerana Order: Hymenoptera	None	0.02%	5.0
Rickettsia typhi Species-level Match	RISB1906	Bemisia tabaci Order: Hemiptera	None	0.02%	5.0
Snodgrassella alvi Species-level Match	RISB1947	Apis cerana Order: Hymenoptera	None	0.01%	5.0
Rickettsia prowazekii Species-level Match	RISB1905	Bemisia tabaci Order: Hemiptera	None	0.01%	5.0
Cupriavidus pauculus Species-level Match	RISB0694	Alydus tomentosus Order: Hemiptera	None	0.01%	5.0
Candidatus Legionella polyplacis Species-level Match	RISB1687	Polyplax serrata Order: Phthiraptera	None	0.01%	5.0
Zymomonas mobilis Species-level Match	RISB1326	Vespa mandarinia Order: Hymenoptera	None	0.01%	5.0
Deinococcus	RISB1649	Camponotus japonicus Order: Hymenoptera	Four new aminoglycolipids, deinococcucins A–D, were discovered from a Deinococcus sp. strain isolated from the gut of queen carpenter ants, Camponotus japonicus, showed functional ability of inducing the quinone reductase production in host cells	0.03%	5.0
Apibacter	RISB0603	Apis cerana Order: Hymenoptera	The acquisition of genes for the degradation of the toxic monosaccharides potentiates Apibacter with the ability to utilize the pollen hydrolysis products, at the same time enabling monosaccharide detoxification for the host	0.35%	4.8
Burkholderia	RISB1729	Lagria hirta Order: Coleoptera	the symbionts inhibit the growth of antagonistic fungi on the eggs of the insect host, indicating that the Lagria-associated Burkholderia have evolved from plant pathogenic ancestors into insect defensive mutualists	0.09%	4.4
Burkholderia	RISB0402	Riptortus pedestris Order: Hemiptera	symbiont colonization induces the development of the midgut crypts via finely regulating the enterocyte cell cycles, enabling it to stably and abundantly colonize the generated spacious crypts of the bean bug host	0.09%	4.4
Sphingobium	RISB1837	Dendroctonus valens Order: Coleoptera	It can trongly degrade naringenin, and pinitol, the main soluble carbohydrate of P. tabuliformis, is retained in L. procerum-infected phloem and facilitate naringenin biodegradation by the microbiotas.	0.02%	4.0
Weissella	RISB1982	Blattella germanica Order: Blattodea	gut microbiota contributes to production of VCAs that act as fecal aggregation agents and that cockroaches discriminate among the complex odors that emanate from a diverse microbial community	0.03%	3.9
Photorhabdus	RISB2532	Manduca sexta Order: Lepidoptera	produces a small-molecule antibiotic (E)-1,3-dihydroxy-2-(isopropyl)-5-(2-phenylethenyl)benzene (ST) that also acts as an inhibitor of phenoloxidase (PO) in the insect host Manduca sexta.	0.05%	3.8
Bifidobacterium	RISB0616	Spodoptera frugiperda Order: Lepidoptera	Strain wkB204 grew in the presence of amygdalin as the sole carbon source, suggesting that this strain degrades amygdalin and is not susceptible to the potential byproducts	0.04%	3.5
Pectobacterium	RISB1889	Pseudococcus longispinus Order: Hemiptera	a nested symbiotic arrangement, where one bacterium lives inside another bacterium,occurred in building the mosaic metabolic pathways seen in mitochondria and plastids	0.05%	3.4
Candidatus Blochmanniella	RISB2542	Camponotus Order: Hymenoptera	Blochmannia provide essential amino acids to its host,Camponotus floridanus, and that it may also play a role in nitrogen recycling via its functional urease	0.09%	3.2
Candidatus Blochmanniella	RISB1827	Camponotus floridanus Order: Hymenoptera	a modulation of immune gene expression which may facilitate tolerance towards the endosymbionts and thus may contribute to their transovarial transmission	0.09%	3.2
Rhizobium	RISB0135	Coccinella septempunctata Order: Coleoptera	be commonly found in plant roots and they all have nitrogen fixation abilities	1.61%	3.2
Bacteroides	RISB0256	Leptocybe invasa Order: Hymenoptera	Differences in Male-Killing Rickettsia Bacteria between Lineages of the Invasive Gall-Causing Pest Leptocybe invasa	0.82%	3.1
Streptococcus	RISB2625	Galleria mellonella Order: Lepidoptera	suppress bacteria ingested with food by producing bacteriocin and by releasing a lysozyme like enzyme	0.98%	3.0
Bacteroides	RISB0090	Hyphantria cunea Order: Lepidoptera	enhance the compatibility of invasive pests to new hosts and enable more rapid adaptation to new habitats.	0.82%	2.9
Bacteroides	RISB1183	Oryzaephilus surinamensis Order: Coleoptera	supplement precursors for the cuticle synthesis and thereby enhance desiccation resistance of its host	0.82%	2.9
Candidatus Blochmanniella	RISB2448	Camponotus floridanus Order: Hymenoptera	nutritional contribution of the bacteria to host metabolism by production of essential amino acids and urease-mediated nitrogen recycling	0.09%	2.8
Photorhabdus	RISB2573	Manduca sexta Order: Lepidoptera	the bacteria are symbiotic with entomopathogenic nematodes but become pathogenic on release from the nematode into the insect blood system	0.05%	2.8
Weissella	RISB0641	Formica Order: Hymenoptera	exhibited abilities in catabolizing sugars (sucrose, trehalose, melezitose and raffinose) known to be constituents of hemipteran honeydew	0.03%	2.8
Bartonella	RISB1673	Apis mellifera Order: Hymenoptera	a gut symbiont of insects and that the adaptation to blood-feeding insects facilitated colonization of the mammalian bloodstream	0.13%	2.7
Streptococcus	RISB2624	Reticulitermes flavipes Order: Blattodea	can be broken down into substances such as carbon dioxide, ammonia and acetic acid	0.98%	2.6
Yersinia	RISB0492	Cimex hemipterus Order: Hemiptera	the disruption of the abundant Yersinia possibly could be related to the enhanced susceptibility towards the insecticides	0.07%	2.5
Pseudonocardia	RISB0947	Acromyrmex Order: Hymenoptera	Pseudonocardia in the Acromyrmex leaf-cutter ants as a protective partner against the entomopathogenic fungus Metarhizium	0.02%	2.4
Nocardia	RISB0947	Acromyrmex Order: Hymenoptera	Pseudonocardia in the Acromyrmex leaf-cutter ants as a protective partner against the entomopathogenic fungus Metarhizium	0.01%	2.4
Candidatus Cardinium	RISB2290	Sogatella furcifera Order: Hemiptera	dual infection with Cardinium and Wolbachia induced strong cytoplasmic incompatibility (CI) in a single host	0.23%	2.4
Liberibacter	RISB2310	Bactericerca cockerelli Order: Hemiptera	manipulate plant signaling and defensive responses, suppress accumulation of defense transcripts like JA and SA	0.02%	2.3
Blautia	RISB0091	Hyphantria cunea Order: Lepidoptera	enhance the compatibility of invasive pests to new hosts and enable more rapid adaptation to new habitats.	0.10%	2.2
Streptococcus	RISB2604	Homona magnanima Order: Lepidoptera	influence the growth of Bacillus thuringiensis in the larvae	0.98%	2.2
Coprococcus	RISB0092	Hyphantria cunea Order: Lepidoptera	enhance the compatibility of invasive pests to new hosts and enable more rapid adaptation to new habitats.	0.02%	2.1
Pseudonocardia	RISB1218	Mycocepurus smithii Order: Hymenoptera	produce secondary metabolites with antibiotic activity that protects the fungus garden against pathogens	0.02%	2.1
Nocardia	RISB1218	Mycocepurus smithii Order: Hymenoptera	produce secondary metabolites with antibiotic activity that protects the fungus garden against pathogens	0.01%	2.1
Nitrosospira	RISB0869	Sirex noctilio Order: Hymenoptera	might be involved in degrading organic matter and fixing nitrogen occurred exclusively in the larval gut	0.01%	2.1
Halomonas	RISB1808	Monochamus galloprovincialis Order: Coleoptera	Have the ability for degradation of cellulose, proteins and starch	0.63%	2.0
Lachnospira	RISB2110	Blattella germanica Order: Blattodea	Hydrolyze polysaccharide; assist digestion; synthesize acetate, propionate, and butyrate	0.02%	1.8
Nostoc	RISB0812	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-18 oxidation pathway	0.25%	1.7
Liberibacter	RISB2524	Bactericera cockerelli Order: Hemiptera	Reduced expression of plant defensive gene in tomato probably for psyllid success	0.02%	1.6
Bradyrhizobium	RISB0135	Coccinella septempunctata Order: Coleoptera	be commonly found in plant roots and they all have nitrogen fixation abilities	0.01%	1.6
Leuconostoc	RISB0812	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-18 oxidation pathway	0.06%	1.5
Kosakonia	RISB0810	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-16 oxidation pathway	0.01%	1.4
Variovorax	RISB2153	Osmia bicornis Order: Hymenoptera	may be essential to support Osmia larvae in their nutrient uptake	0.02%	1.3
Paraclostridium	RISB0028	Sesamia inferens Order: Lepidoptera	degrade Chlorpyrifos and Chlorantraniliprole in vitro	0.17%	1.2
Pectobacterium	RISB0798	Pseudoregma bambucicola Order: Hemiptera	may help P. bambucicola feed on the stalks of bamboo	0.05%	1.1
Dickeya	RISB1086	Rhodnius prolixus Order: Hemiptera	supply enzymatic biosynthesis of B-complex vitamins	0.05%	1.1
Neokomagataea	RISB1560	Oecophylla smaragdina Order: Hymenoptera	may be related with the formic acid production	0.04%	1.0
Brevibacterium	RISB0464	Acrida cinerea Order: Orthoptera	correlated with the hemicellulose digestibility	0.02%	1.0
Liberibacter	RISB2333	Cacopsylla pyri Order: Hemiptera	behaves as an endophyte rather than a pathogen	0.02%	0.9
Aeromonas	RISB2456	Bombyx mori Order: Lepidoptera	able to utilize the CMcellulose and xylan	0.07%	0.9
Brevibacterium	RISB2359	Bombyx mori Order: Lepidoptera	producing lipase in a gut environment	0.02%	0.8
Curtobacterium	RISB1910	Hyles euphorbiae Order: Lepidoptera	able to degrade alkaloids and/or latex	0.01%	0.8
Nocardioides	RISB1914	Hyles euphorbiae Order: Lepidoptera	able to degrade alkaloids and/or latex	0.01%	0.8
Priestia	RISB0839	Helicoverpa armigera Order: Lepidoptera	producing amylase	0.37%	0.7
Mycobacterium	RISB1156	Nicrophorus concolor Order: Coleoptera	produces Antimicrobial compounds	0.02%	0.7
Halomonas	RISB1374	Bemisia tabaci Order: Hemiptera	None	0.63%	0.6
Turicibacter	RISB0451	Odontotaenius disjunctus Order: Coleoptera	degrading ellulose and xylan	0.02%	0.6
Treponema	RISB0169	Reticulitermes flaviceps Order: Blattodea	None	0.51%	0.5
Aeromonas	RISB1145	Tenebrio molitor Order: Coleoptera	degrading plastics	0.07%	0.4
Kosakonia	RISB1155	Tenebrio molitor Order: Coleoptera	degrading plastics	0.01%	0.4
Apibacter	RISB0604	Apis cerana Order: Hymenoptera	None	0.35%	0.4
Bombilactobacillus	RISB0617	Spodoptera frugiperda Order: Lepidoptera	degrade amygdalin	0.01%	0.4
Helicobacter	RISB0662	Melanaphis bambusae Order: Hemiptera	None	0.19%	0.2
Neisseria	RISB0512	Plutella xylostella Order: Lepidoptera	None	0.12%	0.1
Metabacillus	RISB0902	Myzus persicae Order: Hemiptera	None	0.10%	0.1
Yersinia	RISB0407	Anaphes nitens Order: Hymenoptera	None	0.07%	0.1
Sediminibacterium	RISB0244	Spodoptera frugiperda Order: Lepidoptera	None	0.05%	0.1
Bifidobacterium	RISB1944	Apis cerana Order: Hymenoptera	None	0.04%	0.0
Candidatus Profftia	RISB1664	Adelgidae Order: Hemiptera	None	0.04%	0.0
Dysgonomonas	RISB1481	Brachinus elongatulus Order: Coleoptera	None	0.03%	0.0
Weissella	RISB1566	Liometopum apiculatum Order: Hymenoptera	None	0.03%	0.0
Candidatus Phytoplasma	RISB1620	Cacopsylla pyricola Order: Hemiptera	None	0.03%	0.0
Cedecea	RISB0504	Plutella xylostella Order: Lepidoptera	None	0.02%	0.0
Brevibacterium	RISB0897	Myzus persicae Order: Hemiptera	None	0.02%	0.0
Candidatus Arthromitus	RISB2613	Multiple species Order: None	None	0.02%	0.0
Curtobacterium	RISB0900	Myzus persicae Order: Hemiptera	None	0.01%	0.0
Weeksella	RISB1265	Rheumatobates bergrothi Order: Hemiptera	None	0.01%	0.0