SRR5940736 - Chrysomya megacephala

Basic Information

Run: SRR5940736

Assay Type: WGS

Bioproject: PRJNA385554

Biosample: SAMN07135651

Bytes: 841453701

Center Name: NANYANG TECHNOLOGICAL UNIVERSITY

Sequencing Information

Instrument: Illumina HiSeq 2500

Library Layout: PAIRED

Library Selection: RANDOM

Platform: ILLUMINA

Geographic Information

Country: Singapore

Continent: Asia

Location Name: Singapore

Latitude/Longitude: 1.344170 N 103.679283 E

Sample Information

Host: Chrysomya megacephala

Isolation: NTU campus

Biosample Model: Metagenome or environmental

Collection Date: 2014-12-01

Taxonomic Classification

Potential Symbionts

About Potential Symbionts

This table shows potential symbiont identified in the metagenome sample. Matches are scored based on:

Relative abundance in the sample
Species-level matches with known symbionts
Host insect order matches with reference records
Completeness and richness of functional records

Note: only the three highest scoring comparison records were retained for each symbiont species or genus in the sample

Based on our current records database, this section aims to identify potential functional symbionts in this metagenome sample, with scoring based on:

Relative abundance in sample
Species-level matches with known symbionts
Host insect order matches
Functional record completeness

Note: Showing top 3 highest scoring records for each species/genus

Symbiont Name	Record	Host Species	Function	Abundance	Score Score Composition: Symbiont Abundance Species match bonus Host match bonus Function richness Higher scores indicate stronger symbiotic relationship potential
Morganella morganii Species-level Match Host Order Match	RISB0772	Delia antiqua Order: Diptera	showed significant volatile inhibition activity against fungal entomopathogen Fusarium moniliforme, Botryosphaeria dothidea and both Fusarium oxysporum respectively	4.21%	22.5
Morganella morganii Species-level Match Host Order Match	RISB0008	Phormia regina Order: Diptera	deterred oviposition by female stable flies; The flies' oviposition decisions appear to be guided by bacteria-derived semiochemicals as the bacteria	4.21%	22.2
Morganella morganii Species-level Match Host Order Match	RISB0611	Bactrocera dorsalis Order: Diptera	may hydrolysing nitrogenous waste and providing metabolizable nitrogen for B. dorsalis	4.21%	20.9
Wolbachia pipientis Species-level Match Host Order Match	RISB0766	Aedes fluviatilis Order: Diptera	The presence of Wolbachia pipientis improves energy performance in A. fluviatilis cells; it affects the regulation of key energy sources such as lipids, proteins, and carbohydrates, making the distribution of actin more peripheral and with extensions that come into contact with neighboring cells.	0.86%	20.9
Lactococcus lactis Species-level Match Host Order Match	RISB0131	Ceratitis capitata Order: Diptera	The intestinal microbiota structure was significantly influenced by the probiotic treatment while still maintaining a stable core dominant community of Enterobacteriacea. The colony with these microbiome had the most improved potential functions in terms of gut microbes as well as the carbohydrates active enzymes most improved potential functions.	0.15%	20.2
Listeria monocytogenes Species-level Match Host Order Match	RISB2308	Drosophila melanogaster Order: Diptera	L. monocytogenes infection disrupts host energy metabolism by depleting energy stores (triglycerides and glycogen) and reducing metabolic pathway activity (beta-oxidation and glycolysis). The infection affects antioxidant defense by reducing uric acid levels and alters amino acid metabolism. These metabolic changes are accompanied by melanization, potentially linked to decreased tyrosine levels.	0.05%	20.1
Acinetobacter sp. ESL0695 Species-level Match Host Order Match	RISB2083	Aedes aegypti Order: Diptera	axenic larvae cannot develop	3.15%	18.7
Wolbachia pipientis Species-level Match Host Order Match	RISB1515	Drosophila melanogaster Order: Diptera	increases the recombination rate observed across two genomic intervals and increases the efficacy of natural selection in hosts	0.86%	18.4
Acinetobacter guillouiae Species-level Match Host Order Match	RISB0768	Delia antiqua Order: Diptera	showed significant volatile inhibition activity against fungal entomopathogen Fusarium moniliforme, Botryosphaeria dothidea and both Fusarium oxysporum respectively	0.05%	18.3
Citrobacter freundii Species-level Match Host Order Match	RISB1221	Delia antiqua Order: Diptera	six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana through symbiotic bacterium-derived organic acids	0.53%	18.2
Lactococcus lactis Species-level Match Host Order Match	RISB0113	Bactrocera dorsalis Order: Diptera	increase the resistance of B. dorsalis to β-cypermethrin by regulating cytochrome P450 (P450) enzymes and α-glutathione S-transferase (GST) activities	0.15%	18.2
Wolbachia pipientis Species-level Match Host Order Match	RISB1354	Drosophila melanogaster Order: Diptera	Wolbachia influence octopamine metabolism in the Drosophila females, which is by the symbiont genotype	0.86%	17.9
Proteus sp. ZN5 Species-level Match Host Order Match	RISB2315	Aedes aegypti Order: Diptera	upregulates AMP gene expression, resulting in suppression of DENV infection in the mosquito gut epithelium	0.70%	17.8
Enterococcus faecalis Species-level Match Host Order Match	RISB1411	Bactrocera dorsalis Order: Diptera	female Bactrocera dorsalis fed Enterococcus faecalis and Klebsiella oxytoca enriched diets lived longer but had lower fecundity	0.11%	17.7
Citrobacter freundii Species-level Match Host Order Match	RISB1396	Delia antiqua Order: Diptera	suppressed Beauveria bassiana conidia germination and hyphal growth	0.53%	16.9
Providencia rettgeri Species-level Match Host Order Match	RISB1001	Anastrepha obliqua Order: Diptera	improve the sexual competitiveness of males	0.34%	16.2
Enterococcus faecalis Species-level Match Host Order Match	RISB0095	Bactrocera minax Order: Diptera	egrade phenols in unripe citrus in B. minax larvae	0.11%	16.1
Citrobacter freundii Species-level Match Host Order Match	RISB1162	Bactrocera dorsalis Order: Diptera	Promote the growth of larvae	0.53%	16.1
Bacillus thuringiensis Species-level Match Host Order Match	RISB0820	Simulium tani Order: Diptera	show resistance to some antibiotics	0.25%	16.0
Providencia rettgeri Species-level Match Host Order Match	RISB1169	Bactrocera dorsalis Order: Diptera	Promote the growth of larvae	0.34%	15.9
Lactococcus lactis Species-level Match Host Order Match	RISB1167	Bactrocera dorsalis Order: Diptera	Promote the growth of larvae	0.15%	15.7
Acinetobacter sp. TR3 Species-level Match Host Order Match	RISB2083	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.06%	15.6
Chryseobacterium sp. POL2 Species-level Match Host Order Match	RISB2092	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.06%	15.6
Bacillus cereus Species-level Match Host Order Match	RISB1872	Aedes aegypti Order: Diptera	gut microbiome	0.31%	15.6
Providencia alcalifaciens Species-level Match Host Order Match	RISB1168	Bactrocera dorsalis Order: Diptera	Promote the growth of larvae	0.02%	15.6
Buchnera aphidicola Species-level Match Host Order Match	RISB0051	Episyrphus balteatus Order: Diptera	None	0.46%	15.5
Escherichia coli Species-level Match Host Order Match	RISB1769	Calliphoridae Order: Diptera	None	0.43%	15.4
Staphylococcus hominis Species-level Match Host Order Match	RISB1881	Aedes aegypti Order: Diptera	gut microbiome	0.04%	15.3
Bacillus cereus Species-level Match Host Order Match	RISB1701	Phlebotomus papatasi Order: Diptera	None	0.31%	15.3
Klebsiella pneumoniae Species-level Match Host Order Match	RISB1771	Muscidae Order: Diptera	None	0.19%	15.2
Enterobacter hormaechei Species-level Match Host Order Match	RISB1331	Zeugodacus cucurbitae Order: Diptera	None	0.13%	15.1
Spiroplasma Host Order Match	RISB1796	Drosophila neotestacea Order: Diptera	when parasitized by the nematode Howardula aoronymphium, Spiroplasma encodes a ribosome-inactivating protein (RIP) related to Shiga-like toxins from enterohemorrhagic Escherichia coli and that Howardula ribosomal RNA (rRNA) is depurinated during Spiroplasma-mediated protection of D. neotestacea	0.07%	15.1
Pseudomonas Host Order Match	RISB0425	Anopheles sinensis Order: Diptera	Pseudomonas is the most prevalent microbiota in the Plasmodium-negative groups and protects mosquitoes from the invasion of malaria parasites.A low proportion of the Psuedomonas population of microbiome profiles in the hyperendemic areas, indicating that there might be some factors such as malaria parasites to disturb the balance of microbiota	0.05%	15.1
Lactobacillus Host Order Match	RISB1866	Drosophila melanogaster Order: Diptera	The bacterial cells may thus be able to ameliorate the pH of the acidic region, by the release of weak bases.Additionally, the bacteria have a complex relationship with physiological processes which may affect ionic homeostasis in the gut, such as nutrition and immune function	0.04%	15.0
Spiroplasma Host Order Match	RISB1926	Anopheles gambiae Order: Diptera	may have reproductive interactions with their mosquito hosts，either providing an indirect fitness advantage to females by inducing male killing or by directly protecting the host against natural pathogens	0.07%	14.2
Spiroplasma Host Order Match	RISB2026	Drosophila hydei Order: Diptera	Spiroplasma protect their host against parasitoid attack. The Spiroplasma-conferred protection is partial and flies surviving a wasp attack have reduced adult longevity and fecundity	0.07%	13.7
Pseudomonas Host Order Match	RISB2061	Scaptomyza nigrita Order: Diptera	increased S. nigrita herbivory in bittercress，adult S. nigrita feeding was promoted by a P. fluorescens group strain, while larval feeding was promoted by a P. syringae group strain	0.05%	13.7
Paenibacillus Host Order Match	RISB0774	Delia antiqua Order: Diptera	showed significant contact inhibition activity against fungal entomopathogen Fusarium moniliforme, Botryosphaeria dothidea and both Fusarium oxysporum respectively	0.12%	13.4
Pseudomonas Host Order Match	RISB1512	Delia lupini Order: Diptera	Antibiotic-treated larvae suffered growth retardation on a diet containing plant extract or swainsonine. Gut bacteria showed toxin-degradation activities in vitro	0.05%	13.3
Sphingobacterium Host Order Match	RISB1226	Delia antiqua Order: Diptera	six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana through symbiotic bacterium-derived organic acids	0.11%	12.8
Lactobacillus Host Order Match	RISB0185	Drosophila melanogaster Order: Diptera	enhancing the brain levels of tyrosine decarboxylase 2 (Tdc2), which is an enzyme that synthesizes octopamine (OA)	0.04%	12.3
Lactobacillus Host Order Match	RISB1714	Drosophila melanogaster Order: Diptera	It has the potential to reduce IMI-induced susceptibility to infection.	0.04%	11.5
Sphingobacterium Host Order Match	RISB1400	Delia antiqua Order: Diptera	suppressed Beauveria bassiana conidia germination and hyphal growth	0.11%	11.5
Vagococcus Host Order Match	RISB0042	Aldrichina grahami Order: Diptera	None	0.80%	10.8
Paenibacillus Host Order Match	RISB2098	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.12%	10.7
Buchnera aphidicola Species-level Match	RISB0236	Acyrthosiphon pisum Order: Hemiptera	Buchnera the nutritional endosymbiont of A. pisum is located inside of bacteriocytes and requires aspartate from the aphid host, because it cannot make it de novo. Further Buchnera needs aspartate for the biosynthesis of the essential amino acids lysine and threonine, which the aphid and Buchnera require for survival	0.46%	10.5
Peribacillus Host Order Match	RISB1877	Aedes aegypti Order: Diptera	gut microbiome	0.05%	10.3
Buchnera aphidicola Species-level Match	RISB2485	Macrosiphum euphorbiae Order: Hemiptera	symbiont expression patterns differ between aphid clones with differing levels of virulence, and are influenced by the aphids' host plant. Potentially, symbionts may contribute to differential adaptation of aphids to host plant resistance	0.46%	10.2
Klebsiella pneumoniae Species-level Match	RISB2185	Scirpophaga incertulas Order: Lepidoptera	The ability of these arthropods to feed on wood, foliage and detritus is likely to involve catalysis by different types of cellulases/hemicellulases that are secreted by gut microbiota to digest the structural and recalcitrant lignocellulosic residues in their foods.	0.19%	10.2
Myroides Host Order Match	RISB0626	Musca altica Order: Diptera	None	0.13%	10.1
Microbacterium arborescens Species-level Match	RISB2191	Scirpophaga incertulas Order: Lepidoptera	The ability of these arthropods to feed on wood, foliage and detritus is likely to involve catalysis by different types of cellulases/hemicellulases that are secreted by gut microbiota to digest the structural and recalcitrant lignocellulosic residues in their foods.	0.05%	10.1
Escherichia coli Species-level Match	RISB1339	Manduca sexta Order: Lepidoptera	modulate immunity-related gene expression in the infected F0 larvae, and also in their offspring, triggered immune responses in the infected host associated with shifts in both DNA methylation and histone acetylation	0.43%	9.8
Clostridium sp. DL-VIII Species-level Match	RISB2301	Pyrrhocoris apterus Order: Hemiptera	could play an important role for the insect by degrading complex dietary components, providing nutrient supplementation, or detoxifying noxious chemicals (e.g. cyclopropenoic fatty acids or gossypol) in the diet	0.03%	9.3
Clostridium sp. OS1-26 Species-level Match	RISB2301	Pyrrhocoris apterus Order: Hemiptera	could play an important role for the insect by degrading complex dietary components, providing nutrient supplementation, or detoxifying noxious chemicals (e.g. cyclopropenoic fatty acids or gossypol) in the diet	0.02%	9.2
Streptomyces sp. NBC_01324 Species-level Match	RISB0943	Polybia plebeja Order: Hymenoptera	this bacterium produces antimicrobial compounds that are active against Hirsutella citriformis, a natural fungal enemy of its host, and the human pathogens Staphylococcus aureus and Candida albicans	0.21%	9.2
Mammaliicoccus sciuri Species-level Match	RISB0075	Bombyx mori Order: Lepidoptera	could produce a secreted chitinolytic lysozyme (termed Msp1) to damage fungal cell walls，completely inhibit the spore germination of fungal entomopathogens Metarhizium robertsii and Beauveria bassiana	0.09%	9.1
Streptomyces sp. P3 Species-level Match	RISB0943	Polybia plebeja Order: Hymenoptera	this bacterium produces antimicrobial compounds that are active against Hirsutella citriformis, a natural fungal enemy of its host, and the human pathogens Staphylococcus aureus and Candida albicans	0.06%	9.0
Staphylococcus xylosus Species-level Match	RISB2497	Anticarsia gemmatalis Order: Lepidoptera	allow the adaptation of this insect to plants rich in protease inhibitors, minimizing the potentially harmful consequences of protease inhibitors from some of this insect host plants, such as soybean	0.03%	9.0
Streptomyces sp. SJL17-4 Species-level Match	RISB0943	Polybia plebeja Order: Hymenoptera	this bacterium produces antimicrobial compounds that are active against Hirsutella citriformis, a natural fungal enemy of its host, and the human pathogens Staphylococcus aureus and Candida albicans	0.04%	9.0
Escherichia coli Species-level Match	RISB0128	Tribolium castaneum Order: Coleoptera	may produce 4,8-dimethyldecanal (DMD) production that is strongly associated with attraction to females and host pheromone communication	0.43%	8.2
Blattabacterium cuenoti Species-level Match	RISB0133	Panesthiinae Order: Blattodea	enables hosts to subsist on a nutrient-poor diet; endosymbiont genome erosions are associated with repeated host transitions to an underground life	0.20%	8.1
Proteus vulgaris Species-level Match	RISB0001	Leptinotarsa decemlineata Order: Coleoptera	produces toxic hydrogen cyanide (HCN) and a mandelonitrile-producing cyanoglucoside, amygdalin, which protect the insect from predation	0.33%	8.0
Enterococcus faecalis Species-level Match	RISB0497	Cryptolestes ferrugineus Order: Coleoptera	bacteria can degrade malathion, pirimiphos-methyl, and deltamethrin and utilize these insecticides as the carbon source in vitro.	0.11%	7.7
Staphylococcus epidermidis Species-level Match	RISB1070	Oryctes rhinoceros Order: Coleoptera	gut microbe	1.96%	7.2
Microbacterium arborescens Species-level Match	RISB1759	Spodoptera frugiperda Order: Lepidoptera	degradation of lambda-cyhalothrin, deltamethrin, chlorpyrifos ethyl, lufenuron and spinosyn	0.05%	6.9
Proteus vulgaris Species-level Match	RISB2460	Bombyx mori Order: Lepidoptera	degradation of cellulose, xylan, pectin and starch	0.33%	6.3
Klebsiella pneumoniae Species-level Match	RISB2459	Bombyx mori Order: Lepidoptera	degradation of cellulose, xylan, pectin and starch	0.19%	6.2
Microbacterium arborescens Species-level Match	RISB1761	Spodoptera frugiperda Order: Lepidoptera	may influence the metabolization of pesticides in insects	0.05%	6.2
Blattabacterium cuenoti Species-level Match	RISB0518	Cryptocercus punctulatus Order: Blattodea	collaborative arginine biosynthesis	0.20%	5.9
Blattabacterium cuenoti Species-level Match	RISB0093	Blattella germanica Order: Blattodea	obligate endosymbiont	0.20%	5.6
Paenibacillus	RISB2195	Termitidae Order: Blattodea	The ability of these arthropods to feed on wood, foliage and detritus is likely to involve catalysis by different types of cellulases/hemicellulases that are secreted by gut microbiota to digest the structural and recalcitrant lignocellulosic residues in their foods.	0.12%	5.1
Salmonella enterica Species-level Match	RISB0413	Melanaphis sacchari Order: Hemiptera	None	0.12%	5.1
Flavobacterium johnsoniae Species-level Match	RISB0659	Melanaphis bambusae Order: Hemiptera	None	0.12%	5.1
Candidatus Erwinia haradaeae Species-level Match	RISB1632	Lachninae Order: Hemiptera	None	0.06%	5.1
Candidatus Karelsulcia muelleri Species-level Match	RISB1591	Philaenus spumarius Order: Hemiptera	None	0.02%	5.0
Treponema	RISB2377	termite Order: Blattodea	when grown together, two termite-gut Treponema species influence each other's gene expression in a far more comprehensive and nuanced manner than might have been predicted based on the results of previous studies on the respective pure cultures	0.03%	4.9
Sphingobacterium	RISB2227	Leptinotarsa decemlineata Order: Coleoptera	Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)	0.11%	3.5
Candidatus Blochmanniella	RISB2542	Camponotus Order: Hymenoptera	Blochmannia provide essential amino acids to its host,Camponotus floridanus, and that it may also play a role in nitrogen recycling via its functional urease	0.03%	3.2
Candidatus Blochmanniella	RISB1827	Camponotus floridanus Order: Hymenoptera	a modulation of immune gene expression which may facilitate tolerance towards the endosymbionts and thus may contribute to their transovarial transmission	0.03%	3.1
Tsukamurella	RISB1531	Hoplothrips carpathicus Order: Thysanoptera	This genus was identified as dominant in intensively feeding second-stage larvae and suggests a mechanism by which L2 larvae might process cellulose.	0.05%	3.0
Candidatus Blochmanniella	RISB2448	Camponotus floridanus Order: Hymenoptera	nutritional contribution of the bacteria to host metabolism by production of essential amino acids and urease-mediated nitrogen recycling	0.03%	2.8
Streptococcus	RISB2625	Galleria mellonella Order: Lepidoptera	suppress bacteria ingested with food by producing bacteriocin and by releasing a lysozyme like enzyme	0.41%	2.4
Bacteroides	RISB0256	Leptocybe invasa Order: Hymenoptera	Differences in Male-Killing Rickettsia Bacteria between Lineages of the Invasive Gall-Causing Pest Leptocybe invasa	0.08%	2.4
Bacteroides	RISB0090	Hyphantria cunea Order: Lepidoptera	enhance the compatibility of invasive pests to new hosts and enable more rapid adaptation to new habitats.	0.08%	2.2
Bacteroides	RISB1183	Oryzaephilus surinamensis Order: Coleoptera	supplement precursors for the cuticle synthesis and thereby enhance desiccation resistance of its host	0.08%	2.1
Streptococcus	RISB2624	Reticulitermes flavipes Order: Blattodea	can be broken down into substances such as carbon dioxide, ammonia and acetic acid	0.41%	2.1
Bradyrhizobium	RISB0135	Coccinella septempunctata Order: Coleoptera	be commonly found in plant roots and they all have nitrogen fixation abilities	0.06%	1.6
Streptococcus	RISB2604	Homona magnanima Order: Lepidoptera	influence the growth of Bacillus thuringiensis in the larvae	0.41%	1.6
Vibrio	RISB1810	Monochamus galloprovincialis Order: Coleoptera	Have the ability for degradation of cellulose, proteins and starch	0.12%	1.4
Paraclostridium	RISB0028	Sesamia inferens Order: Lepidoptera	degrade Chlorpyrifos and Chlorantraniliprole in vitro	0.09%	1.2
Lysinibacillus	RISB1416	Psammotermes hypostoma Order: Blattodea	isolates showed significant cellulolytic activity	0.06%	1.0
Curtobacterium	RISB1910	Hyles euphorbiae Order: Lepidoptera	able to degrade alkaloids and/or latex	0.10%	0.9
Priestia	RISB0839	Helicoverpa armigera Order: Lepidoptera	producing amylase	0.16%	0.5
Lysinibacillus	RISB1066	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.06%	0.3
Curtobacterium	RISB0900	Myzus persicae Order: Hemiptera	None	0.10%	0.1
Helicobacter	RISB0662	Melanaphis bambusae Order: Hemiptera	None	0.06%	0.1
Legionella	RISB1687	Polyplax serrata Order: Phthiraptera	None	0.04%	0.0
Weeksella	RISB1265	Rheumatobates bergrothi Order: Hemiptera	None	0.04%	0.0
Treponema	RISB0169	Reticulitermes flaviceps Order: Blattodea	None	0.03%	0.0