SRR5350643 - Harmonia axyridis

Basic Information

Run: SRR5350643

Assay Type: WGS

Bioproject: PRJNA377714

Biosample: SAMN25047105

Bytes: 712254643

Center Name: EMBRAPA

Sequencing Information

Instrument: Illumina HiSeq 2500

Library Layout: PAIRED

Library Selection: RANDOM

Platform: ILLUMINA

Geographic Information

Country: Brazil

Continent: South America

Location Name: Brazil: Distrito Federal

Latitude/Longitude: 15.73 S 47.9002 W

Sample Information

Host: Harmonia axyridis

Isolation: -

Biosample Model: Metagenome or environmental

Collection Date: 2015

Taxonomic Classification

Potential Symbionts

About Potential Symbionts

This table shows potential symbiont identified in the metagenome sample. Matches are scored based on:

Relative abundance in the sample
Species-level matches with known symbionts
Host insect order matches with reference records
Completeness and richness of functional records

Note: only the three highest scoring comparison records were retained for each symbiont species or genus in the sample

Based on our current records database, this section aims to identify potential functional symbionts in this metagenome sample, with scoring based on:

Relative abundance in sample
Species-level matches with known symbionts
Host insect order matches
Functional record completeness

Note: Showing top 3 highest scoring records for each species/genus

Symbiont Name	Record	Host Species	Function	Abundance	Score Score Composition: Symbiont Abundance Species match bonus Host match bonus Function richness Higher scores indicate stronger symbiotic relationship potential
Buchnera aphidicola Species-level Match	RISB0236	Acyrthosiphon pisum Order: Hemiptera	Buchnera the nutritional endosymbiont of A. pisum is located inside of bacteriocytes and requires aspartate from the aphid host, because it cannot make it de novo. Further Buchnera needs aspartate for the biosynthesis of the essential amino acids lysine and threonine, which the aphid and Buchnera require for survival	29.39%	39.4
Buchnera aphidicola Species-level Match	RISB2485	Macrosiphum euphorbiae Order: Hemiptera	symbiont expression patterns differ between aphid clones with differing levels of virulence, and are influenced by the aphids' host plant. Potentially, symbionts may contribute to differential adaptation of aphids to host plant resistance	29.39%	39.2
Buchnera aphidicola Species-level Match	RISB0685	Acyrthosiphon pisum Order: Hemiptera	It supplies the host with vitamins and essential amino acids, such as arginine and methionine that aphids cannot synthesize or derive insufficiently from their diet, the phloem sap of plants	29.39%	38.2
Spiroplasma Host Order Match Host Species Match	RISB0343	Harmonia axyridis Order: Coleoptera	female ladybirds co-infected with Hesperomyces harmoniae and Spiroplasma had a significantly lower fecundity and hatchability compared to females with only one or no symbiont	2.14%	35.6
Bacillus sp. 7D3 Species-level Match Host Order Match	RISB1645	Osphranteria coerulescens Order: Coleoptera	The isolate has cellulolytic activity and can hydrolyze CMC, avicel, cellulose and sawdust with broad temperature and pH stability	9.47%	27.1
Bacillus sp. 7D3 Species-level Match Host Order Match	RISB0805	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-8 oxidation pathway	9.47%	25.9
Bacillus sp. 7D3 Species-level Match Host Order Match	RISB1147	Plodia interpunctella Order: Coleoptera	damaged polyethylene (PE) films	9.47%	25.1
Pseudomonas sp. CIP-10 Species-level Match Host Order Match	RISB1622	Dendroctonus valens Order: Coleoptera	volatiles from predominant bacteria regulate the consumption sequence of carbon sources d-pinitol and d-glucose in the fungal symbiont Leptographium procerum, and appear to alleviate the antagonistic effect from the fungus against RTB larvae	1.78%	21.6
Pseudomonas sp. CIP-10 Species-level Match Host Order Match	RISB2224	Leptinotarsa decemlineata Order: Coleoptera	Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)	1.78%	20.1
Acinetobacter sp. TGL-Y2 Species-level Match Host Order Match	RISB0730	Curculio chinensis Order: Coleoptera	Acinetobacter sp. in C. chinensis enriched after treating with saponin, and when incubating bacteria with saponin for 72 h, saponin content significantly decreased from 4.054 to 1.867 mg/mL (by 16S rRNA metagenome sequencing and HPLC)	0.28%	20.0
Serratia liquefaciens Species-level Match Host Order Match	RISB1624	Dendroctonus valens Order: Coleoptera	volatiles from predominant bacteria regulate the consumption sequence of carbon sources d-pinitol and d-glucose in the fungal symbiont Leptographium procerum, and appear to alleviate the antagonistic effect from the fungus against RTB larvae	0.10%	19.9
Lactococcus lactis Species-level Match Host Order Match	RISB0967	Oulema melanopus Order: Coleoptera	contribute to the decomposition of complex carbohydrates, fatty acids, or polysaccharides in the insect gut. It might also contribute to the improvement of nutrient availability.	0.27%	18.8
Escherichia coli Species-level Match Host Order Match	RISB0128	Tribolium castaneum Order: Coleoptera	may produce 4,8-dimethyldecanal (DMD) production that is strongly associated with attraction to females and host pheromone communication	0.90%	18.6
Pseudomonas sp. CIP-10 Species-level Match Host Order Match	RISB0815	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-15 oxidation pathway	1.78%	18.2
Enterococcus faecalis Species-level Match Host Order Match	RISB0497	Cryptolestes ferrugineus Order: Coleoptera	bacteria can degrade malathion, pirimiphos-methyl, and deltamethrin and utilize these insecticides as the carbon source in vitro.	0.31%	17.9
Streptomyces sp. T12 Species-level Match Host Order Match	RISB0777	Copris tripartitus Order: Coleoptera	contribute brood ball hygiene by inhibiting fungal parasites in the environment	1.19%	17.8
Enterococcus faecalis Species-level Match Host Order Match	RISB2042	Harpalus pensylvanicus Order: Coleoptera	E. faecalis facilitate seed consumption by H. pensylvanicus, possibly by contributing digestive enzymes to their host	0.31%	17.7
Acinetobacter sp. TGL-Y2 Species-level Match Host Order Match	RISB0706	Curculio chinensis Order: Coleoptera	facilitate the degradation of tea saponin; genome contains 47 genes relating to triterpenoids degradation	0.28%	17.4
Serratia liquefaciens Species-level Match Host Order Match	RISB1801	Dendroctonus valens Order: Coleoptera	could alleviate or compromise the antagonistic effects of fungi O. minus and L. procerum on RTB larval growth	0.10%	17.3
Serratia marcescens Species-level Match Host Order Match	RISB1295	Nicrophorus vespilloides Order: Coleoptera	producing antibacterial compound Serrawettin W2, which has antibacterial and nematode-inhibiting effects	0.17%	17.3
Lactococcus lactis Species-level Match Host Order Match	RISB1430	Rhynchophorus ferrugineus Order: Coleoptera	promote the development and body mass gain of RPW larvae by improving their nutrition metabolism	0.27%	17.2
Stenotrophomonas maltophilia Species-level Match Host Order Match	RISB0139	Tenebrio molitor Order: Coleoptera	correlated with polyvinyl chloride PVC degradation	1.14%	17.1
Streptomyces sp. CCM_MD2014 Species-level Match Host Order Match	RISB0777	Copris tripartitus Order: Coleoptera	contribute brood ball hygiene by inhibiting fungal parasites in the environment	0.34%	16.9
Enterococcus faecalis Species-level Match Host Order Match	RISB0374	Tribolium castaneum Order: Coleoptera	modulates host phosphine resistance by interfering with the redox system	0.31%	16.8
Acinetobacter sp. TGL-Y2 Species-level Match Host Order Match	RISB0804	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-11 oxidation pathway	0.28%	16.7
Klebsiella pneumoniae Species-level Match Host Order Match	RISB1153	Tenebrio molitor Order: Coleoptera	degrading plastics	0.63%	16.0
Lactococcus lactis Species-level Match Host Order Match	RISB1065	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.27%	15.5
Staphylococcus epidermidis Species-level Match Host Order Match	RISB1070	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.22%	15.4
Staphylococcus hominis Species-level Match Host Order Match	RISB1071	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.09%	15.3
Wolbachia Host Order Match	RISB1452	Octodonta nipae Order: Coleoptera	Wolbachia harbored dominantly in a female than the male adult, while, no significant differences were observed between male and female body parts and tissues	2.12%	15.3
Spiroplasma Host Order Match	RISB1483	Brachinus elongatulus Order: Coleoptera	may manipulate host reproduction (e.g., cause male-killing) or provide resistance to nematodes and/or parasitoid wasps	2.14%	14.5
Wolbachia Host Order Match	RISB2107	Sitophilus zeamais Order: Coleoptera	Wolbachia directly favored weevil fertility and exhibited only mild indirect effects, usually enhancing the SZPE effect	2.12%	14.5
Vibrio Host Order Match	RISB1810	Monochamus galloprovincialis Order: Coleoptera	Have the ability for degradation of cellulose, proteins and starch	3.16%	14.5
Wolbachia Host Order Match	RISB1282	Ips sp. Order: Coleoptera	inducing cytoplasmic incompatibility, resulting in reproductive distortions and hence	2.12%	13.8
Proteus Host Order Match	RISB0001	Leptinotarsa decemlineata Order: Coleoptera	produces toxic hydrogen cyanide (HCN) and a mandelonitrile-producing cyanoglucoside, amygdalin, which protect the insect from predation	0.21%	12.9
Bacteroides Host Order Match	RISB1183	Oryzaephilus surinamensis Order: Coleoptera	supplement precursors for the cuticle synthesis and thereby enhance desiccation resistance of its host	0.79%	12.8
Spiroplasma Host Order Match	RISB0250	Tenebrio molitor Order: Coleoptera	associated with PE biodegradation	2.14%	12.8
Corynebacterium Host Order Match	RISB0363	Pagiophloeus tsushimanus Order: Coleoptera	terpenoid-degrading: the highest degradation rates of D-camphor, linalool, and eucalyptol	0.08%	11.9
Nostoc Host Order Match	RISB0812	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-18 oxidation pathway	0.17%	11.6
Kosakonia Host Order Match	RISB0810	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-16 oxidation pathway	0.09%	11.5
Klebsiella pneumoniae Species-level Match	RISB2185	Scirpophaga incertulas Order: Lepidoptera	The ability of these arthropods to feed on wood, foliage and detritus is likely to involve catalysis by different types of cellulases/hemicellulases that are secreted by gut microbiota to digest the structural and recalcitrant lignocellulosic residues in their foods.	0.63%	10.6
Kosakonia Host Order Match	RISB1155	Tenebrio molitor Order: Coleoptera	degrading plastics	0.09%	10.5
Lysinibacillus Host Order Match	RISB1066	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.23%	10.5
Paenibacillus polymyxa Species-level Match	RISB2195	Termitidae Order: Blattodea	The ability of these arthropods to feed on wood, foliage and detritus is likely to involve catalysis by different types of cellulases/hemicellulases that are secreted by gut microbiota to digest the structural and recalcitrant lignocellulosic residues in their foods.	0.34%	10.3
Escherichia coli Species-level Match	RISB1339	Manduca sexta Order: Lepidoptera	modulate immunity-related gene expression in the infected F0 larvae, and also in their offspring, triggered immune responses in the infected host associated with shifts in both DNA methylation and histone acetylation	0.90%	10.2
Streptomyces sp. T12 Species-level Match	RISB0943	Polybia plebeja Order: Hymenoptera	this bacterium produces antimicrobial compounds that are active against Hirsutella citriformis, a natural fungal enemy of its host, and the human pathogens Staphylococcus aureus and Candida albicans	1.19%	10.2
Stenotrophomonas maltophilia Species-level Match	RISB1122	Bombyx mori Order: Lepidoptera	facilitate host resistance against organophosphate insecticides, provides essential amino acids that increase host fitness and allow the larvae to better tolerate the toxic effects of the insecticide.	1.14%	10.1
Listeria monocytogenes Species-level Match	RISB2308	Drosophila melanogaster Order: Diptera	L. monocytogenes infection disrupts host energy metabolism by depleting energy stores (triglycerides and glycogen) and reducing metabolic pathway activity (beta-oxidation and glycolysis). The infection affects antioxidant defense by reducing uric acid levels and alters amino acid metabolism. These metabolic changes are accompanied by melanization, potentially linked to decreased tyrosine levels.	0.13%	10.1
Blattabacterium cuenoti Species-level Match	RISB0133	Panesthiinae Order: Blattodea	enables hosts to subsist on a nutrient-poor diet; endosymbiont genome erosions are associated with repeated host transitions to an underground life	1.54%	9.5
Clostridium sp. DL-VIII Species-level Match	RISB2301	Pyrrhocoris apterus Order: Hemiptera	could play an important role for the insect by degrading complex dietary components, providing nutrient supplementation, or detoxifying noxious chemicals (e.g. cyclopropenoic fatty acids or gossypol) in the diet	0.14%	9.4
Clostridium sp. OS1-26 Species-level Match	RISB2301	Pyrrhocoris apterus Order: Hemiptera	could play an important role for the insect by degrading complex dietary components, providing nutrient supplementation, or detoxifying noxious chemicals (e.g. cyclopropenoic fatty acids or gossypol) in the diet	0.12%	9.3
Mammaliicoccus sciuri Species-level Match	RISB0075	Bombyx mori Order: Lepidoptera	could produce a secreted chitinolytic lysozyme (termed Msp1) to damage fungal cell walls，completely inhibit the spore germination of fungal entomopathogens Metarhizium robertsii and Beauveria bassiana	0.24%	9.2
Stenotrophomonas maltophilia Species-level Match	RISB1227	Delia antiqua Order: Diptera	six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana through symbiotic bacterium-derived organic acids	1.14%	8.8
Blattabacterium cuenoti Species-level Match	RISB0518	Cryptocercus punctulatus Order: Blattodea	collaborative arginine biosynthesis	1.54%	7.2
Blattabacterium cuenoti Species-level Match	RISB0093	Blattella germanica Order: Blattodea	obligate endosymbiont	1.54%	7.0
Escherichia coli Species-level Match	RISB2120	Galleria mellonella Order: Lepidoptera	mediate trans-generational immune priming	0.90%	6.7
Klebsiella pneumoniae Species-level Match	RISB2459	Bombyx mori Order: Lepidoptera	degradation of cellulose, xylan, pectin and starch	0.63%	6.6
Providencia rettgeri Species-level Match	RISB1001	Anastrepha obliqua Order: Diptera	improve the sexual competitiveness of males	0.38%	6.2
Providencia rettgeri Species-level Match	RISB1169	Bactrocera dorsalis Order: Diptera	Promote the growth of larvae	0.38%	5.9
Chryseobacterium sp. C-71 Species-level Match	RISB2092	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.38%	5.9
Chryseobacterium sp. G0186 Species-level Match	RISB2092	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.37%	5.9
Salmonella enterica Species-level Match	RISB0413	Melanaphis sacchari Order: Hemiptera	None	0.62%	5.6
Francisella	RISB1907	Bombyx mori Order: Lepidoptera	After infection with F. tularensis, the induction of melanization and nodulation, which are immune responses to bacterial infection, were inhibited in silkworms. Pre-inoculation of silkworms with F. tularensis enhanced the expression of antimicrobial peptides and resistance to infection by pathogenic bacteria.	0.58%	5.6
Providencia rettgeri Species-level Match	RISB1352	Nasonia vitripennis Order: Hymenoptera	None	0.38%	5.4
Staphylococcus hominis Species-level Match	RISB1881	Aedes aegypti Order: Diptera	gut microbiome	0.09%	5.4
Candidatus Erwinia haradaeae Species-level Match	RISB1632	Lachninae Order: Hemiptera	None	0.19%	5.2
Candidatus Karelsulcia muelleri Species-level Match	RISB1591	Philaenus spumarius Order: Hemiptera	None	0.13%	5.1
Treponema	RISB2377	termite Order: Blattodea	when grown together, two termite-gut Treponema species influence each other's gene expression in a far more comprehensive and nuanced manner than might have been predicted based on the results of previous studies on the respective pure cultures	0.11%	5.0
Apibacter	RISB0603	Apis cerana Order: Hymenoptera	The acquisition of genes for the degradation of the toxic monosaccharides potentiates Apibacter with the ability to utilize the pollen hydrolysis products, at the same time enabling monosaccharide detoxification for the host	0.10%	4.6
Photorhabdus	RISB2532	Manduca sexta Order: Lepidoptera	produces a small-molecule antibiotic (E)-1,3-dihydroxy-2-(isopropyl)-5-(2-phenylethenyl)benzene (ST) that also acts as an inhibitor of phenoloxidase (PO) in the insect host Manduca sexta.	0.21%	4.0
Streptococcus	RISB2625	Galleria mellonella Order: Lepidoptera	suppress bacteria ingested with food by producing bacteriocin and by releasing a lysozyme like enzyme	1.08%	3.1
Bacteroides	RISB0256	Leptocybe invasa Order: Hymenoptera	Differences in Male-Killing Rickettsia Bacteria between Lineages of the Invasive Gall-Causing Pest Leptocybe invasa	0.79%	3.1
Photorhabdus	RISB2573	Manduca sexta Order: Lepidoptera	the bacteria are symbiotic with entomopathogenic nematodes but become pathogenic on release from the nematode into the insect blood system	0.21%	3.0
Bacteroides	RISB0090	Hyphantria cunea Order: Lepidoptera	enhance the compatibility of invasive pests to new hosts and enable more rapid adaptation to new habitats.	0.79%	2.9
Streptococcus	RISB2624	Reticulitermes flavipes Order: Blattodea	can be broken down into substances such as carbon dioxide, ammonia and acetic acid	1.08%	2.7
Flavobacterium	RISB0659	Melanaphis bambusae Order: Hemiptera	None	2.40%	2.4
Proteus	RISB2315	Aedes aegypti Order: Diptera	upregulates AMP gene expression, resulting in suppression of DENV infection in the mosquito gut epithelium	0.21%	2.3
Streptococcus	RISB2604	Homona magnanima Order: Lepidoptera	influence the growth of Bacillus thuringiensis in the larvae	1.08%	2.3
Corynebacterium	RISB0531	Helicoverpa armigera Order: Lepidoptera	Corynebacterium sp. 2-TD, mediates the toxicity of the 2-tridecanone to H. armigera	0.08%	1.7
Photorhabdus	RISB0532	Drosophila melanogaster Order: Diptera	produces toxin complex (Tc) toxins as major virulence factors	0.21%	1.4
Paraclostridium	RISB0028	Sesamia inferens Order: Lepidoptera	degrade Chlorpyrifos and Chlorantraniliprole in vitro	0.28%	1.3
Proteus	RISB2460	Bombyx mori Order: Lepidoptera	degradation of cellulose, xylan, pectin and starch	0.21%	1.2
Lysinibacillus	RISB1416	Psammotermes hypostoma Order: Blattodea	isolates showed significant cellulolytic activity	0.23%	1.2
Gordonia	RISB1912	Hyles euphorbiae Order: Lepidoptera	able to degrade alkaloids and/or latex	0.21%	1.0
Corynebacterium	RISB2360	Bombyx mori Order: Lepidoptera	producing lipase in a gut environment	0.08%	0.8
Priestia	RISB0839	Helicoverpa armigera Order: Lepidoptera	producing amylase	0.42%	0.8
Peribacillus	RISB1877	Aedes aegypti Order: Diptera	gut microbiome	0.26%	0.5
Helicobacter	RISB0662	Melanaphis bambusae Order: Hemiptera	None	0.21%	0.2
Legionella	RISB1687	Polyplax serrata Order: Phthiraptera	None	0.17%	0.2
Treponema	RISB0169	Reticulitermes flaviceps Order: Blattodea	None	0.11%	0.1
Apibacter	RISB0604	Apis cerana Order: Hymenoptera	None	0.10%	0.1
Myroides	RISB0626	Musca altica Order: Diptera	None	0.10%	0.1