SRR3736777 - Bactrocera oleae

Basic Information

Run: SRR3736777

Assay Type: WGS

Bioproject: PRJNA326914

Biosample: SAMN05294122

Bytes: 442340163

Center Name: UNIVERSITY OF LIVERPOOL

Sequencing Information

Instrument: Illumina MiSeq

Library Layout: PAIRED

Library Selection: RANDOM

Platform: ILLUMINA

Geographic Information

Country: Greece

Continent: Europe

Location Name: Greece:Crete

Latitude/Longitude: 35.35 N 24.45 E

Sample Information

Host: Bactrocera oleae

Isolation: -

Biosample Model: Metagenome or environmental

Collection Date: 2015-12

Taxonomic Classification

Potential Symbionts

About Potential Symbionts

This table shows potential symbiont identified in the metagenome sample. Matches are scored based on:

Relative abundance in the sample
Species-level matches with known symbionts
Host insect order matches with reference records
Completeness and richness of functional records

Note: only the three highest scoring comparison records were retained for each symbiont species or genus in the sample

Based on our current records database, this section aims to identify potential functional symbionts in this metagenome sample, with scoring based on:

Relative abundance in sample
Species-level matches with known symbionts
Host insect order matches
Functional record completeness

Note: Showing top 3 highest scoring records for each species/genus

Symbiont Name	Record	Host Species	Function	Abundance	Score Score Composition: Symbiont Abundance Species match bonus Host match bonus Function richness Higher scores indicate stronger symbiotic relationship potential
Staphylococcus epidermidis Species-level Match	RISB1070	Oryctes rhinoceros Order: Coleoptera	gut microbe	59.81%	65.0
Wolbachia Host Order Match	RISB0766	Aedes fluviatilis Order: Diptera	The presence of Wolbachia pipientis improves energy performance in A. fluviatilis cells; it affects the regulation of key energy sources such as lipids, proteins, and carbohydrates, making the distribution of actin more peripheral and with extensions that come into contact with neighboring cells.	19.37%	34.4
Wolbachia Host Order Match	RISB0779	Drosophila melanogaster Order: Diptera	Wolbachia infection affects differential gene expression in Drosophila testis.Genes involved in carbohydrate metabolism, lysosomal degradation, proteolysis, lipid metabolism, and immune response were upregulated in the presence of Wolbachia	19.37%	34.2
Wolbachia Host Order Match	RISB1408	Anastrepha fraterculus Order: Diptera	Wolbachia is the only known reproductive symbiont present in these morphotypes. Wolbachia reduced the ability for embryonic development in crosses involving cured females and infected males within each morphotype (uni-directional CI).	19.37%	34.1
Klebsiella michiganensis Species-level Match Host Order Match	RISB1052	Bactrocera dorsalis Order: Diptera	K. michiganensis BD177 has the strain-specific ability to provide three essential amino acids (phenylalanine, tryptophan and methionine) and two vitamins B (folate and riboflavin) to B. dorsalis	0.01%	18.9
Serratia marcescens Species-level Match Host Order Match	RISB1291	Aedes aegypti Order: Diptera	facilitates arboviral infection through a secreted protein named SmEnhancin, which digests membrane-bound mucins on the mosquito gut epithelia, thereby enhancing viral dissemination.	0.07%	18.7
Klebsiella michiganensis Species-level Match Host Order Match	RISB1131	Bactrocera dorsalis Order: Diptera	promotes host resistance to low-temperature stress by stimulating its arginine and proline metabolism pathway in adult Bactrocera dorsalis	0.01%	17.8
Serratia marcescens Species-level Match Host Order Match	RISB0009	Phormia regina Order: Diptera	prompted oviposition by flies; The flies' oviposition decisions appear to be guided by bacteria-derived semiochemicals as the bacteria	0.07%	17.8
Serratia plymuthica Species-level Match Host Order Match	RISB1225	Delia antiqua Order: Diptera	six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana through symbiotic bacterium-derived organic acids	0.02%	17.7
Klebsiella sp. PL-2018 Species-level Match Host Order Match	RISB0917	Aedes aegypti Order: Diptera	could impact larval development (e.g., spermidine)	0.08%	16.1
Erwinia aphidicola Species-level Match Host Order Match	RISB1705	Phlebotomus papatasi Order: Diptera	None	0.57%	15.6
Staphylococcus hominis Species-level Match Host Order Match	RISB1881	Aedes aegypti Order: Diptera	gut microbiome	0.02%	15.3
Paenibacillus Host Order Match	RISB0774	Delia antiqua Order: Diptera	showed significant contact inhibition activity against fungal entomopathogen Fusarium moniliforme, Botryosphaeria dothidea and both Fusarium oxysporum respectively	1.93%	15.2
Escherichia coli Species-level Match Host Order Match	RISB1769	Calliphoridae Order: Diptera	None	0.03%	15.0
Enterobacter Host Order Match	RISB0129	Ceratitis capitata Order: Diptera	The intestinal microbiota structure was significantly influenced by the probiotic treatment while still maintaining a stable core dominant community of Enterobacteriacea. The colony with these microbiome had the most improved potential functions in terms of gut microbes as well as the carbohydrates active enzymes most improved potential functions.	0.01%	15.0
Enterobacter Host Order Match	RISB1338	Ceratitis capitata Order: Diptera	Enterobacter sp. AA26 dry biomass can fully replace the brewer’s yeast as a protein source in medfly larval diet without any effect on the productivity and the biological quality of reared medfly of VIENNA 8 GSS	0.01%	14.2
Enterobacter Host Order Match	RISB1223	Delia antiqua Order: Diptera	six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana through symbiotic bacterium-derived organic acids	0.01%	12.7
Proteus Host Order Match	RISB2315	Aedes aegypti Order: Diptera	upregulates AMP gene expression, resulting in suppression of DENV infection in the mosquito gut epithelium	0.56%	12.7
Paenibacillus Host Order Match	RISB2098	Aedes aegypti Order: Diptera	axenic larvae cannot develop	1.93%	12.5
Proteus Host Order Match	RISB0054	Episyrphus balteatus Order: Diptera	None	0.56%	10.6
Pantoea agglomerans Species-level Match	RISB2197	Termitidae Order: Blattodea	The ability of these arthropods to feed on wood, foliage and detritus is likely to involve catalysis by different types of cellulases/hemicellulases that are secreted by gut microbiota to digest the structural and recalcitrant lignocellulosic residues in their foods.	0.27%	10.3
Vagococcus Host Order Match	RISB0042	Aldrichina grahami Order: Diptera	None	0.18%	10.2
Streptomyces sp. NBC_00358 Species-level Match	RISB0943	Polybia plebeja Order: Hymenoptera	this bacterium produces antimicrobial compounds that are active against Hirsutella citriformis, a natural fungal enemy of its host, and the human pathogens Staphylococcus aureus and Candida albicans	1.06%	10.0
Streptomyces sp. NBC_00358 Species-level Match	RISB2334	Sirex noctilio Order: Hymenoptera	degrading woody substrates and that such degradation may assist in nutrient acquisition by S. noctilio, thus contributing to its ability to be established in forested habitats worldwide	1.06%	9.8
Escherichia coli Species-level Match	RISB1339	Manduca sexta Order: Lepidoptera	modulate immunity-related gene expression in the infected F0 larvae, and also in their offspring, triggered immune responses in the infected host associated with shifts in both DNA methylation and histone acetylation	0.03%	9.4
Spiroplasma sp. SV19 Species-level Match	RISB1353	Cephus cinctus Order: Hymenoptera	The bacterium also encoded biosynthetic pathways for essential vitamins B2, B3, and B9. We identified putative Spiroplasma virulence genes: cardiolipin and chitinase.	0.20%	8.5
Streptomyces sp. NBC_00358 Species-level Match	RISB1134	mud dauber wasp Order: Hymenoptera	secondary metabolites derived from a Streptomyces sp. displayed significant inhibitory activity against hexokinase II	1.06%	8.4
Escherichia coli Species-level Match	RISB0128	Tribolium castaneum Order: Coleoptera	may produce 4,8-dimethyldecanal (DMD) production that is strongly associated with attraction to females and host pheromone communication	0.03%	7.8
Pantoea agglomerans Species-level Match	RISB2579	Schistocerca gregaria Order: Orthoptera	produces an antifungal and antibacterial molecule serving as antimicrobial defense against gut pathogens	0.27%	7.4
Paenibacillus	RISB2195	Termitidae Order: Blattodea	The ability of these arthropods to feed on wood, foliage and detritus is likely to involve catalysis by different types of cellulases/hemicellulases that are secreted by gut microbiota to digest the structural and recalcitrant lignocellulosic residues in their foods.	1.93%	6.9
Erwinia sp. HDF1-3R Species-level Match	RISB0808	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-12 oxidation pathway	0.01%	6.4
Pantoea agglomerans Species-level Match	RISB0379	Frankliniella occidentalis Order: Thysanoptera	gut symbionts are required for their development	0.27%	6.2
Erwinia sp. HDF1-3R Species-level Match	RISB1986	Bombyx mori Order: Lepidoptera	producing cellulase and amylase	0.01%	5.6
Burkholderia	RISB1172	Lagria villosa Order: Coleoptera	process a cryptic gene cluster that codes for the biosynthesis of a novel antifungal polyketide with a glutarimide pharmacophore, which led to the discovery of the gladiofungins as previously-overlooked components of the antimicrobial armory of the beetle symbiont	0.42%	5.4
Staphylococcus hominis Species-level Match	RISB1071	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.02%	5.2
Salmonella enterica Species-level Match	RISB0413	Melanaphis sacchari Order: Hemiptera	None	0.02%	5.0
Burkholderia	RISB1729	Lagria hirta Order: Coleoptera	the symbionts inhibit the growth of antagonistic fungi on the eggs of the insect host, indicating that the Lagria-associated Burkholderia have evolved from plant pathogenic ancestors into insect defensive mutualists	0.42%	4.7
Burkholderia	RISB0402	Riptortus pedestris Order: Hemiptera	symbiont colonization induces the development of the midgut crypts via finely regulating the enterocyte cell cycles, enabling it to stably and abundantly colonize the generated spacious crypts of the bean bug host	0.42%	4.7
Clostridium	RISB2301	Pyrrhocoris apterus Order: Hemiptera	could play an important role for the insect by degrading complex dietary components, providing nutrient supplementation, or detoxifying noxious chemicals (e.g. cyclopropenoic fatty acids or gossypol) in the diet	0.28%	4.5
Symbiopectobacterium	RISB1889	Pseudococcus longispinus Order: Hemiptera	a nested symbiotic arrangement, where one bacterium lives inside another bacterium,occurred in building the mosaic metabolic pathways seen in mitochondria and plastids	0.01%	3.4
Proteus	RISB0001	Leptinotarsa decemlineata Order: Coleoptera	produces toxic hydrogen cyanide (HCN) and a mandelonitrile-producing cyanoglucoside, amygdalin, which protect the insect from predation	0.56%	3.3
Yersinia	RISB0492	Cimex hemipterus Order: Hemiptera	the disruption of the abundant Yersinia possibly could be related to the enhanced susceptibility towards the insecticides	0.34%	2.8
Bacteroides	RISB0256	Leptocybe invasa Order: Hymenoptera	Differences in Male-Killing Rickettsia Bacteria between Lineages of the Invasive Gall-Causing Pest Leptocybe invasa	0.19%	2.5
Bacteroides	RISB0090	Hyphantria cunea Order: Lepidoptera	enhance the compatibility of invasive pests to new hosts and enable more rapid adaptation to new habitats.	0.19%	2.3
Bacteroides	RISB1183	Oryzaephilus surinamensis Order: Coleoptera	supplement precursors for the cuticle synthesis and thereby enhance desiccation resistance of its host	0.19%	2.2
Clostridium	RISB0028	Sesamia inferens Order: Lepidoptera	degrade Chlorpyrifos and Chlorantraniliprole in vitro	0.28%	1.3
Cronobacter	RISB0247	Tenebrio molitor Order: Coleoptera	may be indirectly involved in the digestion of PE	0.02%	1.0
Yersinia	RISB0407	Anaphes nitens Order: Hymenoptera	None	0.34%	0.3
Clostridium	RISB1959	Pyrrhocoridae Order: Hemiptera	None	0.28%	0.3
Cupriavidus	RISB0694	Alydus tomentosus Order: Hemiptera	None	0.01%	0.0