SRR28387197 - simuliidae

Basic Information

Run: SRR28387197

Assay Type: WGS

Bioproject: PRJNA1088476

Biosample: SAMN40472428

Bytes: 741011366

Center Name: KU LEUVEN

Sequencing Information

Instrument: Illumina NovaSeq 6000

Library Layout: PAIRED

Library Selection: RANDOM PCR

Platform: ILLUMINA

Quality Control Information

Filter Percentage: 0.073

QC Average Length: 141

Retained Reads: 14829734

Geographic Information

Country: Cameroon

Continent: Africa

Location Name: Cameroon

Latitude/Longitude: 4.347925 N 11.635309 E

Sample Information

Host: simuliidae

Isolation: -

Biosample Model: Metagenome or environmental

Collection Date: 2021-07

Taxonomic Classification

Potential Symbionts

Based on our current records database, this section aims to identify potential functional symbionts in this metagenome sample, with scoring based on:

  • Relative abundance in sample
  • Species-level matches with known symbionts
  • Host insect order matches
  • Functional record completeness

Note: Showing top 3 highest scoring records for each species/genus

Symbiont Name Record Host Species Function Abundance
Score
Pantoea agglomerans
RISB2197
Termitidae
Order: Blattodea
The ability of these arthropods to feed on wood, foliage and detritus is likely to involve catalysis by different types of cellulases/hemicellulases that are secreted by gut microbiota to digest the structural and recalcitrant lignocellulosic residues in their foods.
22.26%
32.3
Pantoea agglomerans
RISB2579
Schistocerca gregaria
Order: Orthoptera
produces an antifungal and antibacterial molecule serving as antimicrobial defense against gut pathogens
22.26%
29.3
Pantoea agglomerans
RISB0379
Frankliniella occidentalis
Order: Thysanoptera
gut symbionts are required for their development
22.26%
28.2
Stenotrophomonas maltophilia
RISB1227
Delia antiqua
Order: Diptera
six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana through symbiotic bacterium-derived organic acids
4.79%
22.5
Stenotrophomonas maltophilia
RISB1141
Hermetia illucens
Order: Diptera
enhance the insect growth performance when reared on an unbalanced nutritionally poor diet
4.79%
21.6
Enterobacter sp. T2
RISB0893
Bactrocera dorsalis
Order: Diptera
be beneficial, with some quality control indices, such as adult size, pupal weight, survival rate under stress and nutritionally rich conditions, and mating competitiveness, being significantly increased, while slight nonsignificant increases in emergence rate and flight ability were observed
1.31%
21.3
Stenotrophomonas maltophilia
RISB1401
Delia antiqua
Order: Diptera
suppressed Beauveria bassiana conidia germination and hyphal growth
4.79%
21.1
Enterobacter sp. T2
RISB1338
Ceratitis capitata
Order: Diptera
Enterobacter sp. AA26 dry biomass can fully replace the brewer’s yeast as a protein source in medfly larval diet without any effect on the productivity and the biological quality of reared medfly of VIENNA 8 GSS
1.31%
20.5
Klebsiella oxytoca
RISB0130
Ceratitis capitata
Order: Diptera
The intestinal microbiota structure was significantly influenced by the probiotic treatment while still maintaining a stable core dominant community of Enterobacteriacea. The  colony with these microbiome had the most improved potential functions in terms of gut microbes as well as the carbohydrates active enzymes most improved potential functions.
0.28%
20.3
Enterobacter sp. SA187
RISB0893
Bactrocera dorsalis
Order: Diptera
be beneficial, with some quality control indices, such as adult size, pupal weight, survival rate under stress and nutritionally rich conditions, and mating competitiveness, being significantly increased, while slight nonsignificant increases in emergence rate and flight ability were observed
0.03%
20.0
Klebsiella michiganensis
RISB1052
Bactrocera dorsalis
Order: Diptera
K. michiganensis BD177 has the strain-specific ability to provide three essential amino acids (phenylalanine, tryptophan and methionine) and two vitamins B (folate and riboflavin) to B. dorsalis
0.05%
18.9
Serratia marcescens
RISB1291
Aedes aegypti
Order: Diptera
facilitates arboviral infection through a secreted protein named SmEnhancin, which digests membrane-bound mucins on the mosquito gut epithelia, thereby enhancing viral dissemination.
0.28%
18.9
Asaia
RISB0854
Anopheles stephensi
Order: Diptera
Two complete operons encoding cytochrome bo3-type ubiquinol terminal oxidases (cyoABCD-1 and cyoABCD-2) were found in most Asaia genomes, possibly offering alternative terminal oxidases and allowing the flexible transition of respiratory pathways. Genes involved in the production of 2,3-butandiol and inositol have been found in Asaia sp. W12, possibly contributing to biofilm formation and stress tolerance.
3.91%
18.9
Citrobacter sp. RHB25-C09
RISB1503
Bactrocera dorsalis
Order: Diptera
Pesticide-degrading bacteria were frequently detected from pesticide-resistant insects. Susceptible insects became resistant after inoculation of the pesticide-degrading symbiont
0.02%
18.6
Klebsiella pneumoniae
RISB1771
Muscidae
Order: Diptera
None
3.52%
18.5
Bacillus cereus
RISB1872
Aedes aegypti
Order: Diptera
gut microbiome
3.05%
18.3
Morganella morganii
RISB0772
Delia antiqua
Order: Diptera
showed significant volatile inhibition activity against fungal entomopathogen Fusarium moniliforme, Botryosphaeria dothidea and both Fusarium oxysporum respectively
0.03%
18.3
Citrobacter freundii
RISB1221
Delia antiqua
Order: Diptera
six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana through symbiotic bacterium-derived organic acids
0.54%
18.2
Bacillus cereus
RISB1701
Phlebotomus papatasi
Order: Diptera
None
3.05%
18.1
Serratia plymuthica
RISB1225
Delia antiqua
Order: Diptera
six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana through symbiotic bacterium-derived organic acids
0.30%
18.0
Morganella morganii
RISB0008
Phormia regina
Order: Diptera
deterred oviposition by female stable flies; The flies' oviposition decisions appear to be guided by bacteria-derived semiochemicals as the bacteria
0.03%
18.0
Serratia marcescens
RISB0009
Phormia regina
Order: Diptera
prompted oviposition by flies; The flies' oviposition decisions appear to be guided by bacteria-derived semiochemicals as the bacteria
0.28%
18.0
Asaia
RISB0014
Aedes aegypti
Order: Diptera
The bacterium Asaia is considered a highly promising candidate for arboviral control in Aedes mosquitoes.Asaia could play a role in inhibiting CHIKV within Ae. aegypti.
3.91%
17.3
Escherichia coli
RISB1769
Calliphoridae
Order: Diptera
None
1.93%
16.9
Citrobacter freundii
RISB1396
Delia antiqua
Order: Diptera
suppressed Beauveria bassiana conidia germination and hyphal growth
0.54%
16.9
Acetobacter
RISB1865
Drosophila melanogaster
Order: Diptera
The bacterial cells may thus be able to ameliorate the pH of the acidic region, by the release of weak bases.Additionally, the bacteria have a complex relationship with physiological processes which may affect ionic homeostasis in the gut, such as nutrition and immune function
1.84%
16.8
Asaia
RISB2533
Anopheles stephensi
Order: Diptera
Asaia sp. strain effectively lodged in the female gut and salivary glands, sites that are crucial for Plasmodium sp. development and transmission
3.91%
16.8
Morganella morganii
RISB0611
Bactrocera dorsalis
Order: Diptera
may hydrolysing nitrogenous waste and providing metabolizable nitrogen for B. dorsalis
0.03%
16.8
Erwinia aphidicola
RISB1705
Phlebotomus papatasi
Order: Diptera
None
1.69%
16.7
Bacillus thuringiensis
RISB0820
Simulium tani
Order: Diptera
show resistance to some antibiotics
0.05%
15.8
Acinetobacter sp. WY4
RISB2083
Aedes aegypti
Order: Diptera
axenic larvae cannot develop
0.12%
15.7
Chryseobacterium sp.
RISB2092
Aedes aegypti
Order: Diptera
axenic larvae cannot develop
0.06%
15.6
Acetobacter
RISB0961
Drosophila melanogaster
Order: Diptera
The exist of Acetobacter had a balancing effect on food ingestion when carbohydrate levels were high in the warmer months, stabilizing fitness components of flies across the year.
1.84%
15.4
Buchnera aphidicola
RISB0051
Episyrphus balteatus
Order: Diptera
None
0.08%
15.1
Variovorax sp. PBL-E5
RISB1712
Phlebotomus papatasi
Order: Diptera
None
0.03%
15.0
Spiroplasma
RISB1796
Drosophila neotestacea
Order: Diptera
when parasitized by the nematode Howardula aoronymphium, Spiroplasma encodes a ribosome-inactivating protein (RIP) related to Shiga-like toxins from enterohemorrhagic Escherichia coli and that Howardula ribosomal RNA (rRNA) is depurinated during Spiroplasma-mediated protection of D. neotestacea
0.03%
15.0
Pseudomonas sp. CIP-10
RISB1622
Dendroctonus valens
Order: Coleoptera
volatiles from predominant bacteria regulate the consumption sequence of carbon sources d-pinitol and d-glucose in the fungal symbiont Leptographium procerum, and appear to alleviate the antagonistic effect from the fungus against RTB larvae
5.06%
14.9
Acetobacter
RISB0184
Drosophila melanogaster
Order: Diptera
enhancing the brain levels of tyrosine decarboxylase 2 (Tdc2), which is an enzyme that synthesizes octopamine (OA)
1.84%
14.1
Spiroplasma
RISB1926
Anopheles gambiae
Order: Diptera
may have reproductive interactions with their mosquito hosts,either providing an indirect fitness advantage to females by inducing male killing or by directly protecting the host against natural pathogens
0.03%
14.1
Proteus
RISB2315
Aedes aegypti
Order: Diptera
upregulates AMP gene expression, resulting in suppression of DENV infection in the mosquito gut epithelium
1.78%
13.9
Spiroplasma
RISB2026
Drosophila hydei
Order: Diptera
Spiroplasma protect their host against parasitoid attack. The Spiroplasma-conferred protection is partial and flies surviving a wasp attack have reduced adult longevity and fecundity
0.03%
13.7
Pseudomonas sp. CIP-10
RISB2224
Leptinotarsa decemlineata
Order: Coleoptera
Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)
5.06%
13.4
Gluconobacter
RISB0016
Aedes aegypti
Order: Diptera
Gluconobacter might increase the susceptibility of Ae. aegypti to CHIKV infection.
1.12%
12.8
Sphingobacterium
RISB1226
Delia antiqua
Order: Diptera
six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana through symbiotic bacterium-derived organic acids
0.06%
12.8
Gluconobacter
RISB1882
Drosophila suzukii
Order: Diptera
produce volatile substances that attract female D. suzukii
1.12%
12.3
Proteus
RISB0054
Episyrphus balteatus
Order: Diptera
None
1.78%
11.8
Pseudomonas sp. CIP-10
RISB0700
Nilaparvata lugens
Order: Hemiptera
Pseudomonas sp. composition and abundance correlated with BPH survivability
5.06%
11.6
Sphingobacterium
RISB1400
Delia antiqua
Order: Diptera
suppressed Beauveria bassiana conidia germination and hyphal growth
0.06%
11.4
Komagataeibacter
RISB1883
Drosophila suzukii
Order: Diptera
produce volatile substances that attract female D. suzukii
0.17%
11.3
Escherichia coli
RISB1339
Manduca sexta
Order: Lepidoptera
modulate immunity-related gene expression in the infected F0 larvae, and also in their offspring, triggered immune responses in the infected host associated with shifts in both DNA methylation and histone acetylation
1.93%
11.3
Gluconobacter
RISB0876
Drosophila suzukii
Order: Diptera
None
1.12%
11.1
Peribacillus
RISB1877
Aedes aegypti
Order: Diptera
gut microbiome
0.50%
10.8
Cedecea
RISB1570
Bactrocera tau
Order: Diptera
could attract male and female B. tau
0.02%
10.7
Burkholderia gladioli
RISB1172
Lagria villosa
Order: Coleoptera
process a cryptic gene cluster that codes for the biosynthesis of a novel antifungal polyketide with a glutarimide pharmacophore, which led to the discovery of the gladiofungins as previously-overlooked components of the antimicrobial armory of the beetle symbiont
0.09%
10.1
Buchnera aphidicola
RISB0236
Acyrthosiphon pisum
Order: Hemiptera
Buchnera the nutritional endosymbiont of A. pisum is located inside of bacteriocytes and requires aspartate from the aphid host, because it cannot make it de novo. Further Buchnera needs aspartate for the biosynthesis of the essential amino acids lysine and threonine, which the aphid and Buchnera require for survival
0.08%
10.1
Buchnera aphidicola
RISB2485
Macrosiphum euphorbiae
Order: Hemiptera
symbiont expression patterns differ between aphid clones with differing levels of virulence, and are influenced by the aphids' host plant. Potentially, symbionts may contribute to differential adaptation of aphids to host plant resistance
0.08%
9.8
Acinetobacter sp. WY4
RISB0730
Curculio chinensis
Order: Coleoptera
Acinetobacter sp. in C. chinensis enriched after treating with saponin, and when incubating bacteria with saponin for 72 h, saponin content significantly decreased from 4.054 to 1.867 mg/mL (by 16S rRNA metagenome sequencing and HPLC)
0.12%
9.8
Escherichia coli
RISB0128
Tribolium castaneum
Order: Coleoptera
may produce 4,8-dimethyldecanal (DMD) production that is strongly associated with attraction to females and host pheromone communication
1.93%
9.7
Burkholderia gladioli
RISB1729
Lagria hirta
Order: Coleoptera
the symbionts inhibit the growth of antagonistic fungi on the eggs of the insect host, indicating that the Lagria-associated Burkholderia have evolved from plant pathogenic ancestors into insect defensive mutualists
0.09%
9.4
Streptomyces sp. WAC00303
RISB0943
Polybia plebeja
Order: Hymenoptera
this bacterium produces antimicrobial compounds that are active against Hirsutella citriformis, a natural fungal enemy of its host, and the human pathogens Staphylococcus aureus and Candida albicans
0.24%
9.2
Acinetobacter sp. WY4
RISB1978
Blattella germanica
Order: Blattodea
gut microbiota contributes to production of VCAs that act as fecal aggregation agents and that cockroaches discriminate among the complex odors that emanate from a diverse microbial community
0.12%
8.9
Streptomyces sp. WAC00303
RISB2334
Sirex noctilio
Order: Hymenoptera
degrading woody substrates and that such degradation may assist in nutrient acquisition by S. noctilio, thus contributing to its ability to be established in forested habitats worldwide
0.24%
8.9
Burkholderia gladioli
RISB1604
Lagria villosa
Order: Coleoptera
Bacteria produce icosalide, an unusual two-tailed lipocyclopeptide antibiotic,which is active against entomopathogenic bacteria, thus adding to the chemical armory protecting beetle offspring
0.09%
8.9
Blattabacterium cuenoti
RISB0133
Panesthiinae
Order: Blattodea
enables hosts to subsist on a nutrient-poor diet; endosymbiont genome erosions are associated with repeated host transitions to an underground life
0.02%
8.0
Streptomyces sp. WAC00303
RISB1134
mud dauber wasp
Order: Hymenoptera
secondary metabolites derived from a Streptomyces sp. displayed significant inhibitory activity against hexokinase II
0.24%
7.6
Leclercia adecarboxylata
RISB1757
Spodoptera frugiperda
Order: Lepidoptera
degradation of lambda-cyhalothrin, deltamethrin, chlorpyrifos ethyl, lufenuron and spinosyn
0.14%
7.0
Erwinia sp. HDF1-3R
RISB0808
Hypothenemus hampei
Order: Coleoptera
might contribute to caffeine breakdown using the C-12 oxidation pathway
0.25%
6.7
Salmonella enterica
RISB0413
Melanaphis sacchari
Order: Hemiptera
None
1.64%
6.6
Kosakonia sp. BYX6
RISB0810
Hypothenemus hampei
Order: Coleoptera
might contribute to caffeine breakdown using the C-16 oxidation pathway
0.20%
6.6
Erwinia sp. QL-Z3
RISB0808
Hypothenemus hampei
Order: Coleoptera
might contribute to caffeine breakdown using the C-12 oxidation pathway
0.17%
6.6
Leclercia adecarboxylata
RISB1758
Spodoptera frugiperda
Order: Lepidoptera
may influence the metabolization of pesticides in insects
0.14%
6.3
Blattabacterium cuenoti
RISB0518
Cryptocercus punctulatus
Order: Blattodea
collaborative arginine biosynthesis
0.02%
5.7
Zymobacter palmae
RISB1324
Vespa mandarinia
Order: Hymenoptera
None
0.70%
5.7
Blattabacterium cuenoti
RISB0093
Blattella germanica
Order: Blattodea
obligate endosymbiont
0.02%
5.4
Oecophyllibacter saccharovorans
RISB1194
Oecophylla smaragdina
Order: Hymenoptera
None
0.40%
5.4
Formicincola oecophyllae
RISB0578
Oecophylla smaragdina
Order: Hymenoptera
None
0.29%
5.3
Staphylococcus epidermidis
RISB1070
Oryctes rhinoceros
Order: Coleoptera
gut microbe
0.05%
5.3
Candidatus Kirkpatrickella diaphorinae
RISB0222
Diaphorina citri
Order: Hemiptera
None
0.10%
5.1
Rahnella
RISB1623
Dendroctonus valens
Order: Coleoptera
volatiles from predominant bacteria regulate the consumption sequence of carbon sources d-pinitol and d-glucose in the fungal symbiont Leptographium procerum, and appear to alleviate the antagonistic effect from the fungus against RTB larvae
0.05%
4.9
Proteus
RISB0001
Leptinotarsa decemlineata
Order: Coleoptera
produces toxic hydrogen cyanide (HCN) and a mandelonitrile-producing cyanoglucoside, amygdalin, which protect the insect from predation
1.78%
4.5
Halomonas
RISB1808
Monochamus galloprovincialis
Order: Coleoptera
Have the ability for degradation of cellulose, proteins and starch
2.63%
4.0
Weissella
RISB1982
Blattella germanica
Order: Blattodea
gut microbiota contributes to production of VCAs that act as fecal aggregation agents and that cockroaches discriminate among the complex odors that emanate from a diverse microbial community
0.05%
3.9
Xanthomonas
RISB0498
Xylocopa appendiculata
Order: Hymenoptera
Xanthomonas strain from Japanese carpenter bee is effective PU-degradable bacterium and is able to use polyacryl-based PU as a nutritional source, as well as other types of PS-PU and PE-PU
0.03%
3.8
Sphingobacterium
RISB2227
Leptinotarsa decemlineata
Order: Coleoptera
Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)
0.06%
3.4
Amycolatopsis
RISB0483
Trachymyrmex smithi
Order: Hymenoptera
inhibited the growth of Pseudonocardia symbionts under laboratory conditions. The novel analog nocamycin V from the strain was identified as the antibacterial compound
0.04%
3.4
Amycolatopsis
RISB0199
Trachymyrmex
Order: Hymenoptera
produce antibiotic EC0-0501 that has strong activity against ant-associated Actinobacteria and may also play a role in bacterial competition in this niche
0.04%
3.1
Yersinia
RISB0492
Cimex hemipterus
Order: Hemiptera
the disruption of the abundant Yersinia possibly could be related to the enhanced susceptibility towards the insecticides
0.62%
3.0
Weissella
RISB0641
Formica
Order: Hymenoptera
exhibited abilities in catabolizing sugars (sucrose, trehalose, melezitose and raffinose) known to be constituents of hemipteran honeydew
0.05%
2.8
Halomonas
RISB1374
Bemisia tabaci
Order: Hemiptera
None
2.63%
2.6
Rahnella
RISB1800
Dendroctonus valens
Order: Coleoptera
could alleviate or compromise the antagonistic effects of fungi O. minus and L. procerum on RTB larval growth
0.05%
2.2
Rahnella
RISB0741
Dendroctonus ponderosae
Order: Coleoptera
R. aquatilis decreased (−)-α-pinene (38%) and (+)-α-pinene (46%) by 40% and 45% (by GC-MS), respectively
0.05%
2.1
Xanthomonas
RISB0217
Xylocopa appendiculata
Order: Hymenoptera
strains biodegraded polyethylene terephthalate PET powder, broke it into its degradation products
0.03%
2.0
Xenorhabdus
RISB1372
Spodoptera frugiperda
Order: Lepidoptera
the products of the symbiont gene cluster inhibit Spodoptera frugiperda phenoloxidase activity
0.03%
1.9
Corynebacterium
RISB0363
Pagiophloeus tsushimanus
Order: Coleoptera
terpenoid-degrading: the highest degradation rates of D-camphor, linalool, and eucalyptol
0.09%
1.9
Rhizobium
RISB0135
Coccinella septempunctata
Order: Coleoptera
be commonly found in plant roots and they all have nitrogen fixation abilities
0.30%
1.9
Corynebacterium
RISB0531
Helicoverpa armigera
Order: Lepidoptera
Corynebacterium sp. 2-TD, mediates the toxicity of the 2-tridecanone to H. armigera
0.09%
1.8
Xenorhabdus
RISB2270
Acyrthosiphon pisum
Order: Hemiptera
have the gene PIN1 encoding the protease inhibitor protein against aphids
0.03%
1.5
Vibrio
RISB1810
Monochamus galloprovincialis
Order: Coleoptera
Have the ability for degradation of cellulose, proteins and starch
0.12%
1.4
Massilia
RISB2151
Osmia bicornis
Order: Hymenoptera
may be essential to support Osmia larvae in their nutrient uptake
0.04%
1.3
Cronobacter
RISB0247
Tenebrio molitor
Order: Coleoptera
may be indirectly involved in the digestion of PE
0.20%
1.2
Dickeya
RISB1086
Rhodnius prolixus
Order: Hemiptera
supply enzymatic biosynthesis of B-complex vitamins
0.10%
1.1
Neokomagataea
RISB1560
Oecophylla smaragdina
Order: Hymenoptera
may be related with the formic acid production
0.12%
1.0
Corynebacterium
RISB2360
Bombyx mori
Order: Lepidoptera
producing lipase in a gut environment
0.09%
0.9
Yersinia
RISB0407
Anaphes nitens
Order: Hymenoptera
None
0.62%
0.6
Flavobacterium
RISB0659
Melanaphis bambusae
Order: Hemiptera
None
0.13%
0.1
Weissella
RISB1566
Liometopum apiculatum
Order: Hymenoptera
None
0.05%
0.1
Cupriavidus
RISB0694
Alydus tomentosus
Order: Hemiptera
None
0.04%
0.0
Cedecea
RISB0504
Plutella xylostella
Order: Lepidoptera
None
0.02%
0.0
Helicobacter
RISB0662
Melanaphis bambusae
Order: Hemiptera
None
0.02%
0.0

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