SRR28387195 - simuliidae

Basic Information

Run: SRR28387195

Assay Type: WGS

Bioproject: PRJNA1088476

Biosample: SAMN40472430

Bytes: 532718113

Center Name: KU LEUVEN

Sequencing Information

Instrument: Illumina NovaSeq 6000

Library Layout: PAIRED

Library Selection: RANDOM PCR

Platform: ILLUMINA

Geographic Information

Country: Cameroon

Continent: Africa

Location Name: Cameroon

Latitude/Longitude: 4.347925 N 11.635309 E

Sample Information

Host: simuliidae

Isolation: -

Biosample Model: Metagenome or environmental

Collection Date: 2021-07

Taxonomic Classification

Potential Symbionts

About Potential Symbionts

This table shows potential symbiont identified in the metagenome sample. Matches are scored based on:

Relative abundance in the sample
Species-level matches with known symbionts
Host insect order matches with reference records
Completeness and richness of functional records

Note: only the three highest scoring comparison records were retained for each symbiont species or genus in the sample

Based on our current records database, this section aims to identify potential functional symbionts in this metagenome sample, with scoring based on:

Relative abundance in sample
Species-level matches with known symbionts
Host insect order matches
Functional record completeness

Note: Showing top 3 highest scoring records for each species/genus

Symbiont Name	Record	Host Species	Function	Abundance	Score Score Composition: Symbiont Abundance Species match bonus Host match bonus Function richness Higher scores indicate stronger symbiotic relationship potential
Bacillus cereus Species-level Match Host Order Match	RISB1872	Aedes aegypti Order: Diptera	gut microbiome	16.85%	32.1
Bacillus cereus Species-level Match Host Order Match	RISB1701	Phlebotomus papatasi Order: Diptera	None	16.85%	31.9
Zymobacter palmae Species-level Match	RISB1324	Vespa mandarinia Order: Hymenoptera	None	23.57%	28.6
Bacillus cereus Species-level Match	RISB2161	Termitidae Order: Blattodea	The ability of these arthropods to feed on wood, foliage and detritus is likely to involve catalysis by different types of cellulases/hemicellulases that are secreted by gut microbiota to digest the structural and recalcitrant lignocellulosic residues in their foods.	16.85%	26.9
Stenotrophomonas maltophilia Species-level Match Host Order Match	RISB1227	Delia antiqua Order: Diptera	six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana through symbiotic bacterium-derived organic acids	7.20%	24.9
Stenotrophomonas maltophilia Species-level Match Host Order Match	RISB1141	Hermetia illucens Order: Diptera	enhance the insect growth performance when reared on an unbalanced nutritionally poor diet	7.20%	24.0
Stenotrophomonas maltophilia Species-level Match Host Order Match	RISB1401	Delia antiqua Order: Diptera	suppressed Beauveria bassiana conidia germination and hyphal growth	7.20%	23.5
Pseudomonas sp. CIP-10 Species-level Match	RISB1622	Dendroctonus valens Order: Coleoptera	volatiles from predominant bacteria regulate the consumption sequence of carbon sources d-pinitol and d-glucose in the fungal symbiont Leptographium procerum, and appear to alleviate the antagonistic effect from the fungus against RTB larvae	12.58%	22.4
Enterobacter sp. T2 Species-level Match Host Order Match	RISB0893	Bactrocera dorsalis Order: Diptera	be beneficial, with some quality control indices, such as adult size, pupal weight, survival rate under stress and nutritionally rich conditions, and mating competitiveness, being significantly increased, while slight nonsignificant increases in emergence rate and flight ability were observed	1.83%	21.8
Enterobacter sp. T2 Species-level Match Host Order Match	RISB1338	Ceratitis capitata Order: Diptera	Enterobacter sp. AA26 dry biomass can fully replace the brewer’s yeast as a protein source in medfly larval diet without any effect on the productivity and the biological quality of reared medfly of VIENNA 8 GSS	1.83%	21.1
Pseudomonas sp. CIP-10 Species-level Match	RISB2224	Leptinotarsa decemlineata Order: Coleoptera	Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)	12.58%	20.9
Klebsiella oxytoca Species-level Match Host Order Match	RISB0130	Ceratitis capitata Order: Diptera	The intestinal microbiota structure was significantly influenced by the probiotic treatment while still maintaining a stable core dominant community of Enterobacteriacea. The colony with these microbiome had the most improved potential functions in terms of gut microbes as well as the carbohydrates active enzymes most improved potential functions.	0.07%	20.1
Pseudomonas sp. CIP-10 Species-level Match	RISB0700	Nilaparvata lugens Order: Hemiptera	Pseudomonas sp. composition and abundance correlated with BPH survivability	12.58%	19.1
Klebsiella michiganensis Species-level Match Host Order Match	RISB1052	Bactrocera dorsalis Order: Diptera	K. michiganensis BD177 has the strain-specific ability to provide three essential amino acids (phenylalanine, tryptophan and methionine) and two vitamins B (folate and riboflavin) to B. dorsalis	0.07%	19.0
Enterobacter sp. T2 Species-level Match Host Order Match	RISB1311	Ceratitis capitata Order: Diptera	it was shown to have positive effects in rearing efficiency when used as larval probiotics	1.83%	18.6
Klebsiella oxytoca Species-level Match Host Order Match	RISB1139	Musca domestica Order: Diptera	It is associated to newly laid housefly eggs, where it is deposited by the female, and has a role in oviposition as well as protection against potential pathogens	0.07%	18.3
Paenibacillus sp. 481 Species-level Match Host Order Match	RISB0774	Delia antiqua Order: Diptera	showed significant contact inhibition activity against fungal entomopathogen Fusarium moniliforme, Botryosphaeria dothidea and both Fusarium oxysporum respectively	0.05%	18.3
Asaia Host Order Match	RISB0854	Anopheles stephensi Order: Diptera	Two complete operons encoding cytochrome bo3-type ubiquinol terminal oxidases (cyoABCD-1 and cyoABCD-2) were found in most Asaia genomes, possibly offering alternative terminal oxidases and allowing the flexible transition of respiratory pathways. Genes involved in the production of 2,3-butandiol and inositol have been found in Asaia sp. W12, possibly contributing to biofilm formation and stress tolerance.	2.80%	17.8
Enterococcus faecalis Species-level Match Host Order Match	RISB1411	Bactrocera dorsalis Order: Diptera	female Bactrocera dorsalis fed Enterococcus faecalis and Klebsiella oxytoca enriched diets lived longer but had lower fecundity	0.05%	17.6
Asaia Host Order Match	RISB0014	Aedes aegypti Order: Diptera	The bacterium Asaia is considered a highly promising candidate for arboviral control in Aedes mosquitoes.Asaia could play a role in inhibiting CHIKV within Ae. aegypti.	2.80%	16.2
Enterococcus faecalis Species-level Match Host Order Match	RISB0095	Bactrocera minax Order: Diptera	egrade phenols in unripe citrus in B. minax larvae	0.05%	16.1
Acetobacter Host Order Match	RISB1865	Drosophila melanogaster Order: Diptera	The bacterial cells may thus be able to ameliorate the pH of the acidic region, by the release of weak bases.Additionally, the bacteria have a complex relationship with physiological processes which may affect ionic homeostasis in the gut, such as nutrition and immune function	0.82%	15.8
Acinetobacter sp. ESL0695 Species-level Match Host Order Match	RISB2083	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.18%	15.7
Escherichia coli Species-level Match Host Order Match	RISB1769	Calliphoridae Order: Diptera	None	0.72%	15.7
Acinetobacter sp. WY4 Species-level Match Host Order Match	RISB2083	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.15%	15.7
Asaia Host Order Match	RISB2533	Anopheles stephensi Order: Diptera	Asaia sp. strain effectively lodged in the female gut and salivary glands, sites that are crucial for Plasmodium sp. development and transmission	2.80%	15.7
Paenibacillus sp. 481 Species-level Match Host Order Match	RISB2098	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.05%	15.6
Acinetobacter sp. Marseille-Q1620 Species-level Match Host Order Match	RISB2083	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.05%	15.6
Chryseobacterium sp. Species-level Match Host Order Match	RISB2092	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.04%	15.6
Wolbachia Host Order Match	RISB0766	Aedes fluviatilis Order: Diptera	The presence of Wolbachia pipientis improves energy performance in A. fluviatilis cells; it affects the regulation of key energy sources such as lipids, proteins, and carbohydrates, making the distribution of actin more peripheral and with extensions that come into contact with neighboring cells.	0.31%	15.3
Pantoea sp. SO10 Species-level Match Host Order Match	RISB1708	Phlebotomus papatasi Order: Diptera	None	0.16%	15.2
Wolbachia Host Order Match	RISB0779	Drosophila melanogaster Order: Diptera	Wolbachia infection affects differential gene expression in Drosophila testis.Genes involved in carbohydrate metabolism, lysosomal degradation, proteolysis, lipid metabolism, and immune response were upregulated in the presence of Wolbachia	0.31%	15.1
Buchnera aphidicola Species-level Match Host Order Match	RISB0051	Episyrphus balteatus Order: Diptera	None	0.06%	15.1
Brevundimonas sp. MF30-B Species-level Match Host Order Match	RISB1703	Phlebotomus papatasi Order: Diptera	None	0.04%	15.0
Wolbachia Host Order Match	RISB1408	Anastrepha fraterculus Order: Diptera	Wolbachia is the only known reproductive symbiont present in these morphotypes. Wolbachia reduced the ability for embryonic development in crosses involving cured females and infected males within each morphotype (uni-directional CI).	0.31%	15.0
Acetobacter Host Order Match	RISB0961	Drosophila melanogaster Order: Diptera	The exist of Acetobacter had a balancing effect on food ingestion when carbohydrate levels were high in the warmer months, stabilizing fitness components of flies across the year.	0.82%	14.4
Acetobacter Host Order Match	RISB0184	Drosophila melanogaster Order: Diptera	enhancing the brain levels of tyrosine decarboxylase 2 (Tdc2), which is an enzyme that synthesizes octopamine (OA)	0.82%	13.1
Proteus Host Order Match	RISB2315	Aedes aegypti Order: Diptera	upregulates AMP gene expression, resulting in suppression of DENV infection in the mosquito gut epithelium	0.60%	12.7
Shewanella Host Order Match	RISB1924	Anopheles gambiae Order: Diptera	may be related with mediating adaptation to different ecological niches or in shaping specific adult behaviors including mating	0.12%	12.7
Comamonas Host Order Match	RISB2021	Bactrocera dorsalis Order: Diptera	This group in the immature stages may be helping the insects to cope with oxidative stress by supplementing available oxygen.	0.03%	12.5
Halomonas	RISB1808	Monochamus galloprovincialis Order: Coleoptera	Have the ability for degradation of cellulose, proteins and starch	11.09%	12.4
Gluconobacter Host Order Match	RISB0016	Aedes aegypti Order: Diptera	Gluconobacter might increase the susceptibility of Ae. aegypti to CHIKV infection.	0.54%	12.2
Gluconobacter Host Order Match	RISB1882	Drosophila suzukii Order: Diptera	produce volatile substances that attract female D. suzukii	0.54%	11.7
Halomonas	RISB1374	Bemisia tabaci Order: Hemiptera	None	11.09%	11.1
Raoultella Host Order Match	RISB1575	Bactrocera tau Order: Diptera	could attract male and female B. tau	0.04%	10.8
Proteus Host Order Match	RISB0054	Episyrphus balteatus Order: Diptera	None	0.60%	10.6
Gluconobacter Host Order Match	RISB0876	Drosophila suzukii Order: Diptera	None	0.54%	10.5
Peribacillus Host Order Match	RISB1877	Aedes aegypti Order: Diptera	gut microbiome	0.17%	10.5
Pantoea agglomerans Species-level Match	RISB2197	Termitidae Order: Blattodea	The ability of these arthropods to feed on wood, foliage and detritus is likely to involve catalysis by different types of cellulases/hemicellulases that are secreted by gut microbiota to digest the structural and recalcitrant lignocellulosic residues in their foods.	0.36%	10.4
Burkholderia gladioli Species-level Match	RISB1172	Lagria villosa Order: Coleoptera	process a cryptic gene cluster that codes for the biosynthesis of a novel antifungal polyketide with a glutarimide pharmacophore, which led to the discovery of the gladiofungins as previously-overlooked components of the antimicrobial armory of the beetle symbiont	0.35%	10.4
Comamonas Host Order Match	RISB1875	Aedes aegypti Order: Diptera	gut microbiome	0.03%	10.3
Pantoea sp. SO10 Species-level Match	RISB0118	Nezara viridula Order: Hemiptera	plays an important role in interactions between insects and plants and could therefore be considered a valuable target for the development of sustainable pest control strategies;transmitted bacteria impacted plant chemical defenses and were able to degrade toxic plant metabolites, aiding the shield bug in its nutrition	0.16%	10.2
Paenibacillus polymyxa Species-level Match	RISB2195	Termitidae Order: Blattodea	The ability of these arthropods to feed on wood, foliage and detritus is likely to involve catalysis by different types of cellulases/hemicellulases that are secreted by gut microbiota to digest the structural and recalcitrant lignocellulosic residues in their foods.	0.06%	10.1
Buchnera aphidicola Species-level Match	RISB0236	Acyrthosiphon pisum Order: Hemiptera	Buchnera the nutritional endosymbiont of A. pisum is located inside of bacteriocytes and requires aspartate from the aphid host, because it cannot make it de novo. Further Buchnera needs aspartate for the biosynthesis of the essential amino acids lysine and threonine, which the aphid and Buchnera require for survival	0.06%	10.1
Escherichia coli Species-level Match	RISB1339	Manduca sexta Order: Lepidoptera	modulate immunity-related gene expression in the infected F0 larvae, and also in their offspring, triggered immune responses in the infected host associated with shifts in both DNA methylation and histone acetylation	0.72%	10.0
Comamonas Host Order Match	RISB2020	Bactrocera dorsalis Order: Diptera	None	0.03%	10.0
Enterococcus mundtii Species-level Match	RISB1733	Spodoptera littoralis Order: Lepidoptera	actively secretes a stable class IIa bacteriocin (mundticin KS) against invading bacteria, including the opportunistic pathogens E. faecalis and E. casseliflavus, but not against other gut residents, facilitating the normal development of host gut microbiota	0.02%	10.0
Buchnera aphidicola Species-level Match	RISB2485	Macrosiphum euphorbiae Order: Hemiptera	symbiont expression patterns differ between aphid clones with differing levels of virulence, and are influenced by the aphids' host plant. Potentially, symbionts may contribute to differential adaptation of aphids to host plant resistance	0.06%	9.8
Burkholderia gladioli Species-level Match	RISB1729	Lagria hirta Order: Coleoptera	the symbionts inhibit the growth of antagonistic fungi on the eggs of the insect host, indicating that the Lagria-associated Burkholderia have evolved from plant pathogenic ancestors into insect defensive mutualists	0.35%	9.7
Streptomyces sp. WAC00303 Species-level Match	RISB0943	Polybia plebeja Order: Hymenoptera	this bacterium produces antimicrobial compounds that are active against Hirsutella citriformis, a natural fungal enemy of its host, and the human pathogens Staphylococcus aureus and Candida albicans	0.29%	9.3
Burkholderia gladioli Species-level Match	RISB1604	Lagria villosa Order: Coleoptera	Bacteria produce icosalide, an unusual two-tailed lipocyclopeptide antibiotic,which is active against entomopathogenic bacteria, thus adding to the chemical armory protecting beetle offspring	0.35%	9.2
Streptomyces sp. T12 Species-level Match	RISB0943	Polybia plebeja Order: Hymenoptera	this bacterium produces antimicrobial compounds that are active against Hirsutella citriformis, a natural fungal enemy of its host, and the human pathogens Staphylococcus aureus and Candida albicans	0.10%	9.1
Streptomyces sp. GQFP Species-level Match	RISB0943	Polybia plebeja Order: Hymenoptera	this bacterium produces antimicrobial compounds that are active against Hirsutella citriformis, a natural fungal enemy of its host, and the human pathogens Staphylococcus aureus and Candida albicans	0.06%	9.0
Escherichia coli Species-level Match	RISB0128	Tribolium castaneum Order: Coleoptera	may produce 4,8-dimethyldecanal (DMD) production that is strongly associated with attraction to females and host pheromone communication	0.72%	8.4
Blattabacterium cuenoti Species-level Match	RISB0133	Panesthiinae Order: Blattodea	enables hosts to subsist on a nutrient-poor diet; endosymbiont genome erosions are associated with repeated host transitions to an underground life	0.04%	8.0
Salmonella enterica Species-level Match	RISB0413	Melanaphis sacchari Order: Hemiptera	None	0.84%	5.8
Blattabacterium cuenoti Species-level Match	RISB0518	Cryptocercus punctulatus Order: Blattodea	collaborative arginine biosynthesis	0.04%	5.7
Blattabacterium cuenoti Species-level Match	RISB0093	Blattella germanica Order: Blattodea	obligate endosymbiont	0.04%	5.5
Staphylococcus epidermidis Species-level Match	RISB1070	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.06%	5.3
Agrobacterium tumefaciens Species-level Match	RISB0650	Melanaphis bambusae Order: Hemiptera	None	0.27%	5.3
Lactobacillus apis Species-level Match	RISB1556	Apis florea Order: Hymenoptera	None	0.03%	5.0
Candidatus Erwinia haradaeae Species-level Match	RISB1632	Lachninae Order: Hemiptera	None	0.02%	5.0
Oecophyllibacter saccharovorans Species-level Match	RISB1194	Oecophylla smaragdina Order: Hymenoptera	None	0.02%	5.0
Formicincola oecophyllae Species-level Match	RISB0578	Oecophylla smaragdina Order: Hymenoptera	None	0.02%	5.0
Clostridium	RISB2301	Pyrrhocoris apterus Order: Hemiptera	could play an important role for the insect by degrading complex dietary components, providing nutrient supplementation, or detoxifying noxious chemicals (e.g. cyclopropenoic fatty acids or gossypol) in the diet	0.18%	4.4
Caballeronia	RISB0399	Riptortus pedestris Order: Hemiptera	in laboratory conditions, C. jiangsuensis significantly enhanced the development, body size, and reproductive potentials of R. pedestris, compared to individuals with no symbiotic bacteria.	0.06%	3.8
Amycolatopsis	RISB0483	Trachymyrmex smithi Order: Hymenoptera	inhibited the growth of Pseudonocardia symbionts under laboratory conditions. The novel analog nocamycin V from the strain was identified as the antibacterial compound	0.09%	3.4
Raoultella	RISB2226	Leptinotarsa decemlineata Order: Coleoptera	Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)	0.04%	3.4
Proteus	RISB0001	Leptinotarsa decemlineata Order: Coleoptera	produces toxic hydrogen cyanide (HCN) and a mandelonitrile-producing cyanoglucoside, amygdalin, which protect the insect from predation	0.60%	3.3
Amycolatopsis	RISB0199	Trachymyrmex Order: Hymenoptera	produce antibiotic EC0-0501 that has strong activity against ant-associated Actinobacteria and may also play a role in bacterial competition in this niche	0.09%	3.2
Caballeronia	RISB0276	Riptortus pedestris Order: Hemiptera	Gut symbiont resulted in increase in the body size and weight of male adults;increased dispersal capacity of male adults especially for flight	0.06%	2.9
Caballeronia	RISB0530	Anasa tristis Order: Hemiptera	the symbiont Caballeronia prevents successful, long-term establishment of phytopathogenic Serratia marcescens in the squash bug	0.06%	2.6
Pseudonocardia	RISB0947	Acromyrmex Order: Hymenoptera	Pseudonocardia in the Acromyrmex leaf-cutter ants as a protective partner against the entomopathogenic fungus Metarhizium	0.03%	2.5
Bacteroides	RISB0256	Leptocybe invasa Order: Hymenoptera	Differences in Male-Killing Rickettsia Bacteria between Lineages of the Invasive Gall-Causing Pest Leptocybe invasa	0.05%	2.4
Bacteroides	RISB0090	Hyphantria cunea Order: Lepidoptera	enhance the compatibility of invasive pests to new hosts and enable more rapid adaptation to new habitats.	0.05%	2.2
Pseudonocardia	RISB1218	Mycocepurus smithii Order: Hymenoptera	produce secondary metabolites with antibiotic activity that protects the fungus garden against pathogens	0.03%	2.1
Bacteroides	RISB1183	Oryzaephilus surinamensis Order: Coleoptera	supplement precursors for the cuticle synthesis and thereby enhance desiccation resistance of its host	0.05%	2.1
Corynebacterium	RISB0363	Pagiophloeus tsushimanus Order: Coleoptera	terpenoid-degrading: the highest degradation rates of D-camphor, linalool, and eucalyptol	0.08%	1.9
Corynebacterium	RISB0531	Helicoverpa armigera Order: Lepidoptera	Corynebacterium sp. 2-TD, mediates the toxicity of the 2-tridecanone to H. armigera	0.08%	1.7
Rhizobium	RISB0135	Coccinella septempunctata Order: Coleoptera	be commonly found in plant roots and they all have nitrogen fixation abilities	0.17%	1.7
Vibrio	RISB1810	Monochamus galloprovincialis Order: Coleoptera	Have the ability for degradation of cellulose, proteins and starch	0.33%	1.7
Raoultella	RISB1672	Spodoptera frugiperda Order: Lepidoptera	downregulated POX but upregulated trypsin PI in this plant species	0.04%	1.4
Massilia	RISB2151	Osmia bicornis Order: Hymenoptera	may be essential to support Osmia larvae in their nutrient uptake	0.04%	1.3
Diaphorobacter	RISB2150	Osmia bicornis Order: Hymenoptera	may be essential to support Osmia larvae in their nutrient uptake	0.03%	1.3
Clostridium	RISB0028	Sesamia inferens Order: Lepidoptera	degrade Chlorpyrifos and Chlorantraniliprole in vitro	0.18%	1.2
Neokomagataea	RISB1560	Oecophylla smaragdina Order: Hymenoptera	may be related with the formic acid production	0.08%	1.0
Corynebacterium	RISB2360	Bombyx mori Order: Lepidoptera	producing lipase in a gut environment	0.08%	0.8
Flavobacterium	RISB0659	Melanaphis bambusae Order: Hemiptera	None	0.43%	0.4
Priestia	RISB0839	Helicoverpa armigera Order: Lepidoptera	producing amylase	0.04%	0.4
Diaphorobacter	RISB1062	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.03%	0.3
Clostridium	RISB1959	Pyrrhocoridae Order: Hemiptera	None	0.18%	0.2
Cupriavidus	RISB0694	Alydus tomentosus Order: Hemiptera	None	0.04%	0.0
Helicobacter	RISB0662	Melanaphis bambusae Order: Hemiptera	None	0.03%	0.0