SRR28387165 - simuliidae

Basic Information

Run: SRR28387165

Assay Type: WGS

Bioproject: PRJNA1088476

Biosample: SAMN40472402

Bytes: 473748739

Center Name: KU LEUVEN

Sequencing Information

Instrument: Illumina NovaSeq 6000

Library Layout: PAIRED

Library Selection: RANDOM PCR

Platform: ILLUMINA

Geographic Information

Country: Cameroon

Continent: Africa

Location Name: Cameroon

Latitude/Longitude: 4.347925 N 11.635309 E

Sample Information

Host: simuliidae

Isolation: -

Biosample Model: Metagenome or environmental

Collection Date: 2021-07

Taxonomic Classification

Potential Symbionts

About Potential Symbionts

This table shows potential symbiont identified in the metagenome sample. Matches are scored based on:

Relative abundance in the sample
Species-level matches with known symbionts
Host insect order matches with reference records
Completeness and richness of functional records

Note: only the three highest scoring comparison records were retained for each symbiont species or genus in the sample

Based on our current records database, this section aims to identify potential functional symbionts in this metagenome sample, with scoring based on:

Relative abundance in sample
Species-level matches with known symbionts
Host insect order matches
Functional record completeness

Note: Showing top 3 highest scoring records for each species/genus

Symbiont Name	Record	Host Species	Function	Abundance	Score Score Composition: Symbiont Abundance Species match bonus Host match bonus Function richness Higher scores indicate stronger symbiotic relationship potential
Klebsiella pneumoniae Species-level Match Host Order Match	RISB1771	Muscidae Order: Diptera	None	11.07%	26.1
Pantoea dispersa Species-level Match Host Order Match	RISB1413	Bactrocera dorsalis Order: Diptera	causing female Bactrocera dorsalis laid more eggs but had shorter lifespan	6.59%	23.1
Pantoea sp. JZ2 Species-level Match Host Order Match	RISB1708	Phlebotomus papatasi Order: Diptera	None	6.54%	21.5
Klebsiella pneumoniae Species-level Match	RISB2185	Scirpophaga incertulas Order: Lepidoptera	The ability of these arthropods to feed on wood, foliage and detritus is likely to involve catalysis by different types of cellulases/hemicellulases that are secreted by gut microbiota to digest the structural and recalcitrant lignocellulosic residues in their foods.	11.07%	21.1
Enterobacter sp. RHBSTW-00593 Species-level Match Host Order Match	RISB0893	Bactrocera dorsalis Order: Diptera	be beneficial, with some quality control indices, such as adult size, pupal weight, survival rate under stress and nutritionally rich conditions, and mating competitiveness, being significantly increased, while slight nonsignificant increases in emergence rate and flight ability were observed	0.70%	20.7
Enterobacter sp. T2 Species-level Match Host Order Match	RISB0893	Bactrocera dorsalis Order: Diptera	be beneficial, with some quality control indices, such as adult size, pupal weight, survival rate under stress and nutritionally rich conditions, and mating competitiveness, being significantly increased, while slight nonsignificant increases in emergence rate and flight ability were observed	0.50%	20.5
Klebsiella oxytoca Species-level Match Host Order Match	RISB0130	Ceratitis capitata Order: Diptera	The intestinal microbiota structure was significantly influenced by the probiotic treatment while still maintaining a stable core dominant community of Enterobacteriacea. The colony with these microbiome had the most improved potential functions in terms of gut microbes as well as the carbohydrates active enzymes most improved potential functions.	0.49%	20.5
Citrobacter freundii Species-level Match Host Order Match	RISB1221	Delia antiqua Order: Diptera	six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana through symbiotic bacterium-derived organic acids	2.43%	20.1
Enterobacter sp. RHBSTW-00593 Species-level Match Host Order Match	RISB1338	Ceratitis capitata Order: Diptera	Enterobacter sp. AA26 dry biomass can fully replace the brewer’s yeast as a protein source in medfly larval diet without any effect on the productivity and the biological quality of reared medfly of VIENNA 8 GSS	0.70%	19.9
Stenotrophomonas maltophilia Species-level Match Host Order Match	RISB1227	Delia antiqua Order: Diptera	six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana through symbiotic bacterium-derived organic acids	2.05%	19.8
Stenotrophomonas maltophilia Species-level Match Host Order Match	RISB1141	Hermetia illucens Order: Diptera	enhance the insect growth performance when reared on an unbalanced nutritionally poor diet	2.05%	18.9
Citrobacter freundii Species-level Match Host Order Match	RISB1396	Delia antiqua Order: Diptera	suppressed Beauveria bassiana conidia germination and hyphal growth	2.43%	18.8
Serratia marcescens Species-level Match Host Order Match	RISB1291	Aedes aegypti Order: Diptera	facilitates arboviral infection through a secreted protein named SmEnhancin, which digests membrane-bound mucins on the mosquito gut epithelia, thereby enhancing viral dissemination.	0.11%	18.8
Citrobacter sp. RHBSTW-00599 Species-level Match Host Order Match	RISB1503	Bactrocera dorsalis Order: Diptera	Pesticide-degrading bacteria were frequently detected from pesticide-resistant insects. Susceptible insects became resistant after inoculation of the pesticide-degrading symbiont	0.17%	18.7
Paenibacillus sp. 481 Species-level Match Host Order Match	RISB0774	Delia antiqua Order: Diptera	showed significant contact inhibition activity against fungal entomopathogen Fusarium moniliforme, Botryosphaeria dothidea and both Fusarium oxysporum respectively	0.18%	18.4
Morganella morganii Species-level Match Host Order Match	RISB0772	Delia antiqua Order: Diptera	showed significant volatile inhibition activity against fungal entomopathogen Fusarium moniliforme, Botryosphaeria dothidea and both Fusarium oxysporum respectively	0.12%	18.4
Stenotrophomonas maltophilia Species-level Match Host Order Match	RISB1401	Delia antiqua Order: Diptera	suppressed Beauveria bassiana conidia germination and hyphal growth	2.05%	18.4
Sodalis glossinidius Species-level Match Host Order Match	RISB2256	Glossina palpalis Order: Diptera	flies harbouring this symbiont have three times greater probability of being infected by trypanosomes than flies without the symbiont.	0.59%	18.3
Escherichia coli Species-level Match Host Order Match	RISB1769	Calliphoridae Order: Diptera	None	3.10%	18.1
Morganella morganii Species-level Match Host Order Match	RISB0008	Phormia regina Order: Diptera	deterred oviposition by female stable flies; The flies' oviposition decisions appear to be guided by bacteria-derived semiochemicals as the bacteria	0.12%	18.1
Serratia marcescens Species-level Match Host Order Match	RISB0009	Phormia regina Order: Diptera	prompted oviposition by flies; The flies' oviposition decisions appear to be guided by bacteria-derived semiochemicals as the bacteria	0.11%	17.8
Pantoea sp. X85 Species-level Match Host Order Match	RISB1708	Phlebotomus papatasi Order: Diptera	None	2.44%	17.4
Bacillus cereus Species-level Match Host Order Match	RISB1872	Aedes aegypti Order: Diptera	gut microbiome	1.82%	17.1
Sodalis glossinidius Species-level Match Host Order Match	RISB2471	Glossina morsitans Order: Diptera	retains a thiamine ABC transporter (tbpAthiPQ) believed to salvage thiamine	0.59%	17.1
Sodalis glossinidius Species-level Match Host Order Match	RISB2531	Glossina spp. Order: Diptera	quorum sensing primes the oxidative stress response of endosymbiont	0.59%	16.9
Morganella morganii Species-level Match Host Order Match	RISB0611	Bactrocera dorsalis Order: Diptera	may hydrolysing nitrogenous waste and providing metabolizable nitrogen for B. dorsalis	0.12%	16.8
Bacillus cereus Species-level Match Host Order Match	RISB1701	Phlebotomus papatasi Order: Diptera	None	1.82%	16.8
Bacillus sp. 7D3 Species-level Match Host Order Match	RISB0791	Anopheles barbirostris Order: Diptera	without this midgut flora showed delayed development to become adult	0.11%	16.5
Serratia marcescens Species-level Match Host Order Match	RISB0096	Bactrocera minax Order: Diptera	egrade phenols in unripe citrus in B. minax larvae	0.11%	16.1
Cedecea lapagei Species-level Match Host Order Match	RISB1570	Bactrocera tau Order: Diptera	could attract male and female B. tau	0.09%	15.8
Paenibacillus sp. 481 Species-level Match Host Order Match	RISB2098	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.18%	15.7
Acinetobacter sp. WY4 Species-level Match Host Order Match	RISB2083	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.11%	15.7
Acinetobacter sp. ESL0695 Species-level Match Host Order Match	RISB2083	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.07%	15.6
Acinetobacter sp. GSS19 Species-level Match Host Order Match	RISB2083	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.04%	15.6
Erwinia aphidicola Species-level Match Host Order Match	RISB1705	Phlebotomus papatasi Order: Diptera	None	0.20%	15.2
Asaia Host Order Match	RISB0854	Anopheles stephensi Order: Diptera	Two complete operons encoding cytochrome bo3-type ubiquinol terminal oxidases (cyoABCD-1 and cyoABCD-2) were found in most Asaia genomes, possibly offering alternative terminal oxidases and allowing the flexible transition of respiratory pathways. Genes involved in the production of 2,3-butandiol and inositol have been found in Asaia sp. W12, possibly contributing to biofilm formation and stress tolerance.	0.17%	15.2
Buchnera aphidicola Species-level Match Host Order Match	RISB0051	Episyrphus balteatus Order: Diptera	None	0.04%	15.0
Asaia Host Order Match	RISB0014	Aedes aegypti Order: Diptera	The bacterium Asaia is considered a highly promising candidate for arboviral control in Aedes mosquitoes.Asaia could play a role in inhibiting CHIKV within Ae. aegypti.	0.17%	13.5
Asaia Host Order Match	RISB2533	Anopheles stephensi Order: Diptera	Asaia sp. strain effectively lodged in the female gut and salivary glands, sites that are crucial for Plasmodium sp. development and transmission	0.17%	13.1
Shewanella Host Order Match	RISB1924	Anopheles gambiae Order: Diptera	may be related with mediating adaptation to different ecological niches or in shaping specific adult behaviors including mating	0.11%	12.7
Proteus Host Order Match	RISB2315	Aedes aegypti Order: Diptera	upregulates AMP gene expression, resulting in suppression of DENV infection in the mosquito gut epithelium	0.35%	12.5
Escherichia coli Species-level Match	RISB1339	Manduca sexta Order: Lepidoptera	modulate immunity-related gene expression in the infected F0 larvae, and also in their offspring, triggered immune responses in the infected host associated with shifts in both DNA methylation and histone acetylation	3.10%	12.4
Pseudomonas sp. CIP-10 Species-level Match	RISB1622	Dendroctonus valens Order: Coleoptera	volatiles from predominant bacteria regulate the consumption sequence of carbon sources d-pinitol and d-glucose in the fungal symbiont Leptographium procerum, and appear to alleviate the antagonistic effect from the fungus against RTB larvae	1.71%	11.5
Actinomyces Host Order Match	RISB1234	Hermetia illucens Order: Diptera	provides the tools for degrading of a broad range of substrates	0.12%	11.4
Escherichia coli Species-level Match	RISB0128	Tribolium castaneum Order: Coleoptera	may produce 4,8-dimethyldecanal (DMD) production that is strongly associated with attraction to females and host pheromone communication	3.10%	10.8
Burkholderia gladioli Species-level Match	RISB1172	Lagria villosa Order: Coleoptera	process a cryptic gene cluster that codes for the biosynthesis of a novel antifungal polyketide with a glutarimide pharmacophore, which led to the discovery of the gladiofungins as previously-overlooked components of the antimicrobial armory of the beetle symbiont	0.53%	10.5
Peribacillus Host Order Match	RISB1877	Aedes aegypti Order: Diptera	gut microbiome	0.25%	10.5
Proteus Host Order Match	RISB0054	Episyrphus balteatus Order: Diptera	None	0.35%	10.4
Enterococcus mundtii Species-level Match	RISB1733	Spodoptera littoralis Order: Lepidoptera	actively secretes a stable class IIa bacteriocin (mundticin KS) against invading bacteria, including the opportunistic pathogens E. faecalis and E. casseliflavus, but not against other gut residents, facilitating the normal development of host gut microbiota	0.06%	10.1
Pseudomonas sp. CIP-10 Species-level Match	RISB2224	Leptinotarsa decemlineata Order: Coleoptera	Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)	1.71%	10.1
Buchnera aphidicola Species-level Match	RISB0236	Acyrthosiphon pisum Order: Hemiptera	Buchnera the nutritional endosymbiont of A. pisum is located inside of bacteriocytes and requires aspartate from the aphid host, because it cannot make it de novo. Further Buchnera needs aspartate for the biosynthesis of the essential amino acids lysine and threonine, which the aphid and Buchnera require for survival	0.04%	10.0
Pseudomonas sp. LTJR-52 Species-level Match	RISB1622	Dendroctonus valens Order: Coleoptera	volatiles from predominant bacteria regulate the consumption sequence of carbon sources d-pinitol and d-glucose in the fungal symbiont Leptographium procerum, and appear to alleviate the antagonistic effect from the fungus against RTB larvae	0.19%	10.0
Enterococcus mundtii Species-level Match	RISB0476	Spodoptera litura Order: Lepidoptera	The ingestion of bacteria negatively affected the development and nutritional physiology of insect. The bacteria after successful establishment started degrading the gut wall and invaded the haemocoel thereby causing the death of the host.	0.06%	9.8
Burkholderia gladioli Species-level Match	RISB1729	Lagria hirta Order: Coleoptera	the symbionts inhibit the growth of antagonistic fungi on the eggs of the insect host, indicating that the Lagria-associated Burkholderia have evolved from plant pathogenic ancestors into insect defensive mutualists	0.53%	9.8
Buchnera aphidicola Species-level Match	RISB2485	Macrosiphum euphorbiae Order: Hemiptera	symbiont expression patterns differ between aphid clones with differing levels of virulence, and are influenced by the aphids' host plant. Potentially, symbionts may contribute to differential adaptation of aphids to host plant resistance	0.04%	9.8
Streptomyces sp. NBC_00239 Species-level Match	RISB0943	Polybia plebeja Order: Hymenoptera	this bacterium produces antimicrobial compounds that are active against Hirsutella citriformis, a natural fungal enemy of its host, and the human pathogens Staphylococcus aureus and Candida albicans	0.41%	9.4
Burkholderia gladioli Species-level Match	RISB1604	Lagria villosa Order: Coleoptera	Bacteria produce icosalide, an unusual two-tailed lipocyclopeptide antibiotic,which is active against entomopathogenic bacteria, thus adding to the chemical armory protecting beetle offspring	0.53%	9.4
Streptomyces sp. WAC00303 Species-level Match	RISB0943	Polybia plebeja Order: Hymenoptera	this bacterium produces antimicrobial compounds that are active against Hirsutella citriformis, a natural fungal enemy of its host, and the human pathogens Staphylococcus aureus and Candida albicans	0.22%	9.2
Streptomyces sp. NBC_00239 Species-level Match	RISB2334	Sirex noctilio Order: Hymenoptera	degrading woody substrates and that such degradation may assist in nutrient acquisition by S. noctilio, thus contributing to its ability to be established in forested habitats worldwide	0.41%	9.1
Enterococcus mundtii Species-level Match	RISB2494	Anticarsia gemmatalis Order: Lepidoptera	allow the adaptation of this insect to plants rich in protease inhibitors, minimizing the potentially harmful consequences of protease inhibitors from some of this insect host plants, such as soybean	0.06%	9.0
Staphylococcus xylosus Species-level Match	RISB2497	Anticarsia gemmatalis Order: Lepidoptera	allow the adaptation of this insect to plants rich in protease inhibitors, minimizing the potentially harmful consequences of protease inhibitors from some of this insect host plants, such as soybean	0.05%	9.0
Blattabacterium cuenoti Species-level Match	RISB0133	Panesthiinae Order: Blattodea	enables hosts to subsist on a nutrient-poor diet; endosymbiont genome erosions are associated with repeated host transitions to an underground life	0.07%	8.0
Leclercia adecarboxylata Species-level Match	RISB1757	Spodoptera frugiperda Order: Lepidoptera	degradation of lambda-cyhalothrin, deltamethrin, chlorpyrifos ethyl, lufenuron and spinosyn	0.11%	6.9
Staphylococcus xylosus Species-level Match	RISB2247	Anticarsia gemmatalis Order: Lepidoptera	mitigation of the negative effects of proteinase inhibitors produced by the host plant	0.05%	6.8
Xenorhabdus bovienii Species-level Match	RISB2270	Acyrthosiphon pisum Order: Hemiptera	have the gene PIN1 encoding the protease inhibitor protein against aphids	0.15%	6.6
Paenibacillus sp. 481 Species-level Match	RISB0813	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-9 oxidation pathway	0.18%	6.6
Kosakonia sp. SMBL-WEM22 Species-level Match	RISB0810	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-16 oxidation pathway	0.16%	6.6
Leclercia adecarboxylata Species-level Match	RISB1758	Spodoptera frugiperda Order: Lepidoptera	may influence the metabolization of pesticides in insects	0.11%	6.3
Staphylococcus xylosus Species-level Match	RISB2246	Anticarsia gemmatalis Order: Lepidoptera	Against plant-derived protease inhibitor; pest control	0.05%	6.1
Zymobacter palmae Species-level Match	RISB1324	Vespa mandarinia Order: Hymenoptera	None	1.13%	6.1
Blattabacterium cuenoti Species-level Match	RISB0518	Cryptocercus punctulatus Order: Blattodea	collaborative arginine biosynthesis	0.07%	5.8
Blattabacterium cuenoti Species-level Match	RISB0093	Blattella germanica Order: Blattodea	obligate endosymbiont	0.07%	5.5
Rahnella	RISB1623	Dendroctonus valens Order: Coleoptera	volatiles from predominant bacteria regulate the consumption sequence of carbon sources d-pinitol and d-glucose in the fungal symbiont Leptographium procerum, and appear to alleviate the antagonistic effect from the fungus against RTB larvae	0.63%	5.5
Salmonella enterica Species-level Match	RISB0413	Melanaphis sacchari Order: Hemiptera	None	0.14%	5.1
Cedecea lapagei Species-level Match	RISB0504	Plutella xylostella Order: Lepidoptera	None	0.09%	5.1
Lactobacillus apis Species-level Match	RISB1556	Apis florea Order: Hymenoptera	None	0.07%	5.1
Erwinia amylovora Species-level Match	RISB0403	Anaphes nitens Order: Hymenoptera	None	0.06%	5.1
Clostridium	RISB2301	Pyrrhocoris apterus Order: Hemiptera	could play an important role for the insect by degrading complex dietary components, providing nutrient supplementation, or detoxifying noxious chemicals (e.g. cyclopropenoic fatty acids or gossypol) in the diet	0.18%	4.4
Amycolatopsis	RISB0483	Trachymyrmex smithi Order: Hymenoptera	inhibited the growth of Pseudonocardia symbionts under laboratory conditions. The novel analog nocamycin V from the strain was identified as the antibacterial compound	0.11%	3.5
Amycolatopsis	RISB0199	Trachymyrmex Order: Hymenoptera	produce antibiotic EC0-0501 that has strong activity against ant-associated Actinobacteria and may also play a role in bacterial competition in this niche	0.11%	3.2
Proteus	RISB0001	Leptinotarsa decemlineata Order: Coleoptera	produces toxic hydrogen cyanide (HCN) and a mandelonitrile-producing cyanoglucoside, amygdalin, which protect the insect from predation	0.35%	3.1
Halomonas	RISB1808	Monochamus galloprovincialis Order: Coleoptera	Have the ability for degradation of cellulose, proteins and starch	1.61%	2.9
Rahnella	RISB1800	Dendroctonus valens Order: Coleoptera	could alleviate or compromise the antagonistic effects of fungi O. minus and L. procerum on RTB larval growth	0.63%	2.8
Nocardia	RISB0947	Acromyrmex Order: Hymenoptera	Pseudonocardia in the Acromyrmex leaf-cutter ants as a protective partner against the entomopathogenic fungus Metarhizium	0.35%	2.8
Rahnella	RISB0741	Dendroctonus ponderosae Order: Coleoptera	R. aquatilis decreased (−)-α-pinene (38%) and (+)-α-pinene (46%) by 40% and 45% (by GC-MS), respectively	0.63%	2.7
Yersinia	RISB0492	Cimex hemipterus Order: Hemiptera	the disruption of the abundant Yersinia possibly could be related to the enhanced susceptibility towards the insecticides	0.18%	2.6
Nocardia	RISB1218	Mycocepurus smithii Order: Hymenoptera	produce secondary metabolites with antibiotic activity that protects the fungus garden against pathogens	0.35%	2.4
Cronobacter	RISB0247	Tenebrio molitor Order: Coleoptera	may be indirectly involved in the digestion of PE	1.29%	2.3
Vibrio	RISB1810	Monochamus galloprovincialis Order: Coleoptera	Have the ability for degradation of cellulose, proteins and starch	0.42%	1.7
Leuconostoc	RISB0812	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-18 oxidation pathway	0.32%	1.7
Halomonas	RISB1374	Bemisia tabaci Order: Hemiptera	None	1.61%	1.6
Dickeya	RISB1086	Rhodnius prolixus Order: Hemiptera	supply enzymatic biosynthesis of B-complex vitamins	0.37%	1.4
Diaphorobacter	RISB2150	Osmia bicornis Order: Hymenoptera	may be essential to support Osmia larvae in their nutrient uptake	0.04%	1.3
Clostridium	RISB0028	Sesamia inferens Order: Lepidoptera	degrade Chlorpyrifos and Chlorantraniliprole in vitro	0.18%	1.2
Flavobacterium	RISB0659	Melanaphis bambusae Order: Hemiptera	None	0.60%	0.6
Fructobacillus	RISB0638	Formica Order: Hymenoptera	None	0.37%	0.4
Diaphorobacter	RISB1062	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.04%	0.3
Legionella	RISB1687	Polyplax serrata Order: Phthiraptera	None	0.19%	0.2
Clostridium	RISB1959	Pyrrhocoridae Order: Hemiptera	None	0.18%	0.2
Yersinia	RISB0407	Anaphes nitens Order: Hymenoptera	None	0.18%	0.2
Cupriavidus	RISB0694	Alydus tomentosus Order: Hemiptera	None	0.05%	0.1
Helicobacter	RISB0662	Melanaphis bambusae Order: Hemiptera	None	0.04%	0.0