SRR27609048 - Bombus impatiens

Basic Information

Run: SRR27609048

Assay Type: WGS

Bioproject: PRJNA1066198

Biosample: SAMN39482746

Bytes: 226625896

Center Name: UNIVERSITY OF OTTAWA

Sequencing Information

Instrument: Illumina NovaSeq 6000

Library Layout: PAIRED

Library Selection: RANDOM

Platform: ILLUMINA

Geographic Information

Country: Canada

Continent: North America

Location Name: Canada: Ottawa

Latitude/Longitude: 45.42141677 N 75.68148579 W

Sample Information

Host: Bombus impatiens

Isolation: gut

Biosample Model: Metagenome or environmental

Collection Date: 2021-05-27

Taxonomic Classification

Potential Symbionts

About Potential Symbionts

This table shows potential symbiont identified in the metagenome sample. Matches are scored based on:

Relative abundance in the sample
Species-level matches with known symbionts
Host insect order matches with reference records
Completeness and richness of functional records

Note: only the three highest scoring comparison records were retained for each symbiont species or genus in the sample

Based on our current records database, this section aims to identify potential functional symbionts in this metagenome sample, with scoring based on:

Relative abundance in sample
Species-level matches with known symbionts
Host insect order matches
Functional record completeness

Note: Showing top 3 highest scoring records for each species/genus

Symbiont Name	Record	Host Species	Function	Abundance	Score Score Composition: Symbiont Abundance Species match bonus Host match bonus Function richness Higher scores indicate stronger symbiotic relationship potential
Gilliamella apicola Species-level Match Host Order Match	RISB0102	Apis mellifera Order: Hymenoptera	Gilliamella apicola carries the gene for the desaturase FADS2, which is able to metabolize polyunsaturated fatty acids from pollen and synthesize endocannabinoid, a lipogenic neuroactive substance, thereby modulating reward learning and memory in honeybees.	0.38%	20.4
Bifidobacterium asteroides Species-level Match Host Order Match	RISB0174	Apis mellifera Order: Hymenoptera	Bifidobacterium provides complementary demethylation service to promote Gilliamella growth on methylated homogalacturonan, an enriched polysaccharide of pectin. In exchange, Gilliamella shares digestive products with Bifidobacterium, through which a positive interaction is established	0.06%	20.1
Snodgrassella alvi Species-level Match Host Order Match	RISB1423	Bombus spp. Order: Hymenoptera	The bumble bee microbiome slightly increases survivorship when the host is exposed to selenate	0.39%	17.3
Providencia sp. R33 Species-level Match Host Order Match	RISB0984	Nasonia vitripennis Order: Hymenoptera	may highly associated with diapause	0.02%	15.7
Snodgrassella alvi Species-level Match Host Order Match	RISB1947	Apis cerana Order: Hymenoptera	None	0.39%	15.4
Gilliamella apicola Species-level Match Host Order Match	RISB1945	Apis cerana Order: Hymenoptera	None	0.38%	15.4
Arsenophonus nasoniae Species-level Match Host Order Match	RISB0428	Nasonia vitripennis Order: Hymenoptera	male killing	0.02%	15.3
Providencia rettgeri Species-level Match Host Order Match	RISB1352	Nasonia vitripennis Order: Hymenoptera	None	0.18%	15.2
Lactobacillus apis Species-level Match Host Order Match	RISB1556	Apis florea Order: Hymenoptera	None	0.07%	15.1
Arsenophonus nasoniae Species-level Match Host Order Match	RISB0366	Pachycrepoideus vindemmiae Order: Hymenoptera	None	0.02%	15.0
Candidatus Blochmanniella Host Order Match	RISB2542	Camponotus Order: Hymenoptera	Blochmannia provide essential amino acids to its host,Camponotus floridanus, and that it may also play a role in nitrogen recycling via its functional urease	0.03%	13.2
Candidatus Blochmanniella Host Order Match	RISB1827	Camponotus floridanus Order: Hymenoptera	a modulation of immune gene expression which may facilitate tolerance towards the endosymbionts and thus may contribute to their transovarial transmission	0.03%	13.1
Candidatus Blochmanniella Host Order Match	RISB2448	Camponotus floridanus Order: Hymenoptera	nutritional contribution of the bacteria to host metabolism by production of essential amino acids and urease-mediated nitrogen recycling	0.03%	12.8
Yersinia Host Order Match	RISB0407	Anaphes nitens Order: Hymenoptera	None	0.16%	10.2
Buchnera aphidicola Species-level Match	RISB0236	Acyrthosiphon pisum Order: Hemiptera	Buchnera the nutritional endosymbiont of A. pisum is located inside of bacteriocytes and requires aspartate from the aphid host, because it cannot make it de novo. Further Buchnera needs aspartate for the biosynthesis of the essential amino acids lysine and threonine, which the aphid and Buchnera require for survival	0.12%	10.1
Klebsiella oxytoca Species-level Match	RISB0130	Ceratitis capitata Order: Diptera	The intestinal microbiota structure was significantly influenced by the probiotic treatment while still maintaining a stable core dominant community of Enterobacteriacea. The colony with these microbiome had the most improved potential functions in terms of gut microbes as well as the carbohydrates active enzymes most improved potential functions.	0.03%	10.0
Buchnera aphidicola Species-level Match	RISB2485	Macrosiphum euphorbiae Order: Hemiptera	symbiont expression patterns differ between aphid clones with differing levels of virulence, and are influenced by the aphids' host plant. Potentially, symbionts may contribute to differential adaptation of aphids to host plant resistance	0.12%	9.9
Escherichia coli Species-level Match	RISB1339	Manduca sexta Order: Lepidoptera	modulate immunity-related gene expression in the infected F0 larvae, and also in their offspring, triggered immune responses in the infected host associated with shifts in both DNA methylation and histone acetylation	0.13%	9.5
Coriobacterium glomerans Species-level Match	RISB2302	Pyrrhocoris apterus Order: Hemiptera	could play an important role for the insect by degrading complex dietary components, providing nutrient supplementation, or detoxifying noxious chemicals (e.g. cyclopropenoic fatty acids or gossypol) in the diet	0.01%	9.2
Coriobacterium glomerans Species-level Match	RISB2125	Dysdercus fasciatus Order: Hemiptera	Elimination of symbionts by egg-surface sterilization resulted in significantly higher mortality and reduced growth rates, indicating that the microbial community plays an important role for host nutrition	0.01%	9.1
Buchnera aphidicola Species-level Match	RISB0685	Acyrthosiphon pisum Order: Hemiptera	It supplies the host with vitamins and essential amino acids, such as arginine and methionine that aphids cannot synthesize or derive insufficiently from their diet, the phloem sap of plants	0.12%	8.9
Klebsiella oxytoca Species-level Match	RISB2565	Acrolepiopsis assectella Order: Lepidoptera	Klebsiella oxytoca and Bacillus spp. produce the volatile alkyl disulfides present in the fecal pellets, which serve as kairomones to attract the parasitoid Diadromus pulchellus to the moth host	0.03%	8.9
Bifidobacterium asteroides Species-level Match	RISB0616	Spodoptera frugiperda Order: Lepidoptera	Strain wkB204 grew in the presence of amygdalin as the sole carbon source, suggesting that this strain degrades amygdalin and is not susceptible to the potential byproducts	0.06%	8.5
Lactobacillus sp. ESL0785 Species-level Match	RISB0292	Lymantria dispar asiatica Order: Lepidoptera	Beauveria bassiana infection-based assays showed that the mortality of non-axenic L. dispar asiatica larvae was significantly higher than that of axenic larvae at 72 h.	0.08%	8.4
Lactobacillus sp. IBH004 Species-level Match	RISB0292	Lymantria dispar asiatica Order: Lepidoptera	Beauveria bassiana infection-based assays showed that the mortality of non-axenic L. dispar asiatica larvae was significantly higher than that of axenic larvae at 72 h.	0.06%	8.4
Candidatus Mikella endobia Species-level Match	RISB1887	Paracoccus marginatus Order: Hemiptera	a nested symbiotic arrangement, where one bacterium lives inside another bacterium,occurred in building the mosaic metabolic pathways seen in mitochondria and plastids	0.01%	8.4
Candidatus Hoaglandella endobia Species-level Match	RISB1886	Trionymus perrisii Order: Hemiptera	a nested symbiotic arrangement, where one bacterium lives inside another bacterium,occurred in building the mosaic metabolic pathways seen in mitochondria and plastids	0.01%	8.4
Morganella morganii Species-level Match	RISB0772	Delia antiqua Order: Diptera	showed significant volatile inhibition activity against fungal entomopathogen Fusarium moniliforme, Botryosphaeria dothidea and both Fusarium oxysporum respectively	0.04%	8.3
Klebsiella oxytoca Species-level Match	RISB1139	Musca domestica Order: Diptera	It is associated to newly laid housefly eggs, where it is deposited by the female, and has a role in oviposition as well as protection against potential pathogens	0.03%	8.3
Citrobacter freundii Species-level Match	RISB0517	Leptinotarsa decemlineata Order: Coleoptera	affect the cellular and humoral immunity of the insect, increasing its susceptibility to Bacillus thuringiensis var. tenebrionis (morrisoni) (Bt)	0.11%	8.0
Morganella morganii Species-level Match	RISB0008	Phormia regina Order: Diptera	deterred oviposition by female stable flies; The flies' oviposition decisions appear to be guided by bacteria-derived semiochemicals as the bacteria	0.04%	8.0
Morganella morganii Species-level Match	RISB1867	Costelytra zealandica Order: Coleoptera	Female beetles were previously shown to use phenol as their sex pheromone produced by symbiotic bacteria in the accessory or colleterial gland	0.04%	7.9
Escherichia coli Species-level Match	RISB0128	Tribolium castaneum Order: Coleoptera	may produce 4,8-dimethyldecanal (DMD) production that is strongly associated with attraction to females and host pheromone communication	0.13%	7.9
Citrobacter freundii Species-level Match	RISB0127	Tribolium castaneum Order: Coleoptera	may produce 4,8-dimethyldecanal (DMD) production that is strongly associated with attraction to females and host pheromone communication	0.11%	7.8
Citrobacter freundii Species-level Match	RISB1221	Delia antiqua Order: Diptera	six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana through symbiotic bacterium-derived organic acids	0.11%	7.8
Proteus vulgaris Species-level Match	RISB0001	Leptinotarsa decemlineata Order: Coleoptera	produces toxic hydrogen cyanide (HCN) and a mandelonitrile-producing cyanoglucoside, amygdalin, which protect the insect from predation	0.09%	7.8
Serratia plymuthica Species-level Match	RISB1225	Delia antiqua Order: Diptera	six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana through symbiotic bacterium-derived organic acids	0.07%	7.8
Enterobacter asburiae Species-level Match	RISB1700	Plutella xylostella Order: Lepidoptera	play an important role in the breakdown of plant cell walls, detoxification of plant phenolics, and synthesis of amino acids.	0.06%	7.6
Frischella perrara Species-level Match	RISB2028	Diceroprocta semicincta Order: Hemiptera	causes the formation of a scab-like structure on the gut epithelium of its host	0.30%	6.9
Enterobacter asburiae Species-level Match	RISB1695	Plutella xylostella Order: Lepidoptera	participate in the synthesis of host lacking amino acids histidine and threonine	0.06%	6.7
Serratia plymuthica Species-level Match	RISB1399	Delia antiqua Order: Diptera	suppressed Beauveria bassiana conidia germination and hyphal growth	0.07%	6.4
Acinetobacter calcoaceticus Species-level Match	RISB0017	Scirpophaga incertulas Order: Lepidoptera	degrade Chlorpyrifos and Chlorantraniliprole in vitro	0.04%	6.1
Proteus vulgaris Species-level Match	RISB2460	Bombyx mori Order: Lepidoptera	degradation of cellulose, xylan, pectin and starch	0.09%	6.1
Providencia rettgeri Species-level Match	RISB1001	Anastrepha obliqua Order: Diptera	improve the sexual competitiveness of males	0.18%	6.0
Escherichia coli Species-level Match	RISB2120	Galleria mellonella Order: Lepidoptera	mediate trans-generational immune priming	0.13%	6.0
Enterobacter asburiae Species-level Match	RISB1697	Plutella xylostella Order: Lepidoptera	degradation of plant phenolics: catechol	0.06%	5.9
Salmonella enterica Species-level Match	RISB0413	Melanaphis sacchari Order: Hemiptera	None	0.86%	5.9
Bombilactobacillus bombi Species-level Match	RISB0617	Spodoptera frugiperda Order: Lepidoptera	degrade amygdalin	0.29%	5.6
Serratia plymuthica Species-level Match	RISB0203	Belgica antarctica Order: Diptera	None	0.07%	5.1
Candidatus Erwinia haradaeae Species-level Match	RISB1632	Lachninae Order: Hemiptera	None	0.05%	5.1
Pantoea	RISB0118	Nezara viridula Order: Hemiptera	plays an important role in interactions between insects and plants and could therefore be considered a valuable target for the development of sustainable pest control strategies;transmitted bacteria impacted plant chemical defenses and were able to degrade toxic plant metabolites, aiding the shield bug in its nutrition	0.04%	5.0
Candidatus Regiella	RISB1370	Sitobion avenae Order: Hemiptera	Regiella infection decreased the intrinsic rate of increase (rm) of aphids at 25 °C and 28 °C. However, at 31 °C, the effect of Regiella on the rm varied depending on the aphid genotype and density. Thus, the negative effects of this endosymbiont on its host were environmentally dependent.	0.03%	5.0
Candidatus Palibaumannia cicadellinicola Species-level Match	RISB1594	Graphocephala coccinea Order: Hemiptera	None	0.02%	5.0
Coriobacterium glomerans Species-level Match	RISB2143	Pyrrhocoris apterus Order: Hemiptera	None	0.01%	5.0
Pantoea	RISB1715	Dolycoris baccarum Order: Hemiptera	The sterilization-produced aposymbiotic nymphs showed high mortality and no insects reached adulthood. In addition, the Pantoea symbiont was uncultivable outside the insect host, indicating an obligate and intimate host-symbiont association	0.04%	4.8
Rahnella	RISB1623	Dendroctonus valens Order: Coleoptera	volatiles from predominant bacteria regulate the consumption sequence of carbon sources d-pinitol and d-glucose in the fungal symbiont Leptographium procerum, and appear to alleviate the antagonistic effect from the fungus against RTB larvae	0.01%	4.8
Candidatus Regiella	RISB1819	Sitobion avenae Order: Hemiptera	In R. insecticola-infected aphid lines, there were increases in plasticities for developmental times of first and second instar nymphs and for fecundity, showing novel functional roles of bacterial symbionts in plant-insect interactions.	0.03%	4.8
Candidatus Regiella	RISB1363	Sitobion avenae Order: Hemiptera	R. insecticola-infected aphids were more predated by the ladybird Hippodamia variegata irrespective of host plants and did not improve defences against coccinellid predators or metabolic rates on any host plants	0.03%	4.3
Pantoea	RISB1671	Spodoptera frugiperda Order: Lepidoptera	modulate plant defense, downregulated the activity of the plant defensive proteins polyphenol oxidase and trypsin proteinase inhibitors (trypsin PI) but upregulated peroxidase (POX) activity in tomatoresponses	0.04%	4.2
Photorhabdus	RISB2532	Manduca sexta Order: Lepidoptera	produces a small-molecule antibiotic (E)-1,3-dihydroxy-2-(isopropyl)-5-(2-phenylethenyl)benzene (ST) that also acts as an inhibitor of phenoloxidase (PO) in the insect host Manduca sexta.	0.02%	3.8
Pectobacterium	RISB1889	Pseudococcus longispinus Order: Hemiptera	a nested symbiotic arrangement, where one bacterium lives inside another bacterium,occurred in building the mosaic metabolic pathways seen in mitochondria and plastids	0.04%	3.4
Photorhabdus	RISB2573	Manduca sexta Order: Lepidoptera	the bacteria are symbiotic with entomopathogenic nematodes but become pathogenic on release from the nematode into the insect blood system	0.02%	2.8
Shewanella	RISB1924	Anopheles gambiae Order: Diptera	may be related with mediating adaptation to different ecological niches or in shaping specific adult behaviors including mating	0.07%	2.6
Yersinia	RISB0492	Cimex hemipterus Order: Hemiptera	the disruption of the abundant Yersinia possibly could be related to the enhanced susceptibility towards the insecticides	0.16%	2.6
Psychrobacter	RISB1773	Calliphoridae Order: Diptera	it shows physiological adaptation to survival in warmer temperatures and has been previously associated with food spoilage	0.02%	2.5
Rahnella	RISB1800	Dendroctonus valens Order: Coleoptera	could alleviate or compromise the antagonistic effects of fungi O. minus and L. procerum on RTB larval growth	0.01%	2.2
Rahnella	RISB0741	Dendroctonus ponderosae Order: Coleoptera	R. aquatilis decreased (−)-α-pinene (38%) and (+)-α-pinene (46%) by 40% and 45% (by GC-MS), respectively	0.01%	2.1
Xenorhabdus	RISB1372	Spodoptera frugiperda Order: Lepidoptera	the products of the symbiont gene cluster inhibit Spodoptera frugiperda phenoloxidase activity	0.08%	2.0
Vibrio	RISB1810	Monochamus galloprovincialis Order: Coleoptera	Have the ability for degradation of cellulose, proteins and starch	0.24%	1.6
Xenorhabdus	RISB2270	Acyrthosiphon pisum Order: Hemiptera	have the gene PIN1 encoding the protease inhibitor protein against aphids	0.08%	1.5
Nostoc	RISB0812	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-18 oxidation pathway	0.02%	1.4
Dysgonomonas	RISB1235	Hermetia illucens Order: Diptera	provides the tools for degrading of a broad range of substrates	0.02%	1.3
Photorhabdus	RISB0532	Drosophila melanogaster Order: Diptera	produces toxin complex (Tc) toxins as major virulence factors	0.02%	1.2
Pectobacterium	RISB0798	Pseudoregma bambucicola Order: Hemiptera	may help P. bambucicola feed on the stalks of bamboo	0.04%	1.1
Lysinibacillus	RISB1416	Psammotermes hypostoma Order: Blattodea	isolates showed significant cellulolytic activity	0.06%	1.0
Achromobacter	RISB1869	Aedes aegypti Order: Diptera	gut microbiome	0.01%	0.3
Lysinibacillus	RISB1066	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.06%	0.3
Bosea	RISB1702	Phlebotomus papatasi Order: Diptera	None	0.13%	0.1
Pectobacterium	RISB1772	Muscidae Order: Diptera	None	0.04%	0.0
Dysgonomonas	RISB1481	Brachinus elongatulus Order: Coleoptera	None	0.02%	0.0
Achromobacter	RISB0383	Aphis gossypii Order: Hemiptera	None	0.01%	0.0
Candidatus Profftia	RISB1664	Adelgidae Order: Hemiptera	None	0.01%	0.0