SRR25391497 - Diptera

Basic Information

Run: SRR25391497

Assay Type: WGS

Bioproject: PRJNA997598

Biosample: SAMN36683861

Bytes: 752019245

Center Name: USDA ARS

Sequencing Information

Instrument: NextSeq 2000

Library Layout: PAIRED

Library Selection: RANDOM

Platform: ILLUMINA

Geographic Information

Country: USA

Continent: North America

Location Name: USA: Kansas

Latitude/Longitude: 39.18 N 96.57 W

Sample Information

Host: Diptera

Isolation: -

Biosample Model: MIMS.me,MIGS/MIMS/MIMARKS.host-associated

Collection Date: 2022-05

Taxonomic Classification

Potential Symbionts

About Potential Symbionts

This table shows potential symbiont identified in the metagenome sample. Matches are scored based on:

Relative abundance in the sample
Species-level matches with known symbionts
Host insect order matches with reference records
Completeness and richness of functional records

Note: only the three highest scoring comparison records were retained for each symbiont species or genus in the sample

Based on our current records database, this section aims to identify potential functional symbionts in this metagenome sample, with scoring based on:

Relative abundance in sample
Species-level matches with known symbionts
Host insect order matches
Functional record completeness

Note: Showing top 3 highest scoring records for each species/genus

Symbiont Name	Record	Host Species	Function	Abundance	Score Score Composition: Symbiont Abundance Species match bonus Host match bonus Function richness Higher scores indicate stronger symbiotic relationship potential
Wolbachia Host Order Match	RISB0766	Aedes fluviatilis Order: Diptera	The presence of Wolbachia pipientis improves energy performance in A. fluviatilis cells; it affects the regulation of key energy sources such as lipids, proteins, and carbohydrates, making the distribution of actin more peripheral and with extensions that come into contact with neighboring cells.	8.89%	23.9
Wolbachia Host Order Match	RISB0779	Drosophila melanogaster Order: Diptera	Wolbachia infection affects differential gene expression in Drosophila testis.Genes involved in carbohydrate metabolism, lysosomal degradation, proteolysis, lipid metabolism, and immune response were upregulated in the presence of Wolbachia	8.89%	23.7
Wolbachia Host Order Match	RISB1408	Anastrepha fraterculus Order: Diptera	Wolbachia is the only known reproductive symbiont present in these morphotypes. Wolbachia reduced the ability for embryonic development in crosses involving cured females and infected males within each morphotype (uni-directional CI).	8.89%	23.6
Vibrio	RISB1810	Monochamus galloprovincialis Order: Coleoptera	Have the ability for degradation of cellulose, proteins and starch	20.04%	21.4
Lactococcus lactis Species-level Match Host Order Match	RISB0131	Ceratitis capitata Order: Diptera	The intestinal microbiota structure was significantly influenced by the probiotic treatment while still maintaining a stable core dominant community of Enterobacteriacea. The colony with these microbiome had the most improved potential functions in terms of gut microbes as well as the carbohydrates active enzymes most improved potential functions.	0.51%	20.5
Listeria monocytogenes Species-level Match Host Order Match	RISB2308	Drosophila melanogaster Order: Diptera	L. monocytogenes infection disrupts host energy metabolism by depleting energy stores (triglycerides and glycogen) and reducing metabolic pathway activity (beta-oxidation and glycolysis). The infection affects antioxidant defense by reducing uric acid levels and alters amino acid metabolism. These metabolic changes are accompanied by melanization, potentially linked to decreased tyrosine levels.	0.10%	20.1
Paenibacillus sp. PAMC21692 Species-level Match Host Order Match	RISB0774	Delia antiqua Order: Diptera	showed significant contact inhibition activity against fungal entomopathogen Fusarium moniliforme, Botryosphaeria dothidea and both Fusarium oxysporum respectively	0.59%	18.9
Serratia marcescens Species-level Match Host Order Match	RISB1291	Aedes aegypti Order: Diptera	facilitates arboviral infection through a secreted protein named SmEnhancin, which digests membrane-bound mucins on the mosquito gut epithelia, thereby enhancing viral dissemination.	0.12%	18.8
Lactococcus lactis Species-level Match Host Order Match	RISB0113	Bactrocera dorsalis Order: Diptera	increase the resistance of B. dorsalis to β-cypermethrin by regulating cytochrome P450 (P450) enzymes and α-glutathione S-transferase (GST) activities	0.51%	18.5
Morganella morganii Species-level Match Host Order Match	RISB0772	Delia antiqua Order: Diptera	showed significant volatile inhibition activity against fungal entomopathogen Fusarium moniliforme, Botryosphaeria dothidea and both Fusarium oxysporum respectively	0.09%	18.4
Paenibacillus sp. RC67 Species-level Match Host Order Match	RISB0774	Delia antiqua Order: Diptera	showed significant contact inhibition activity against fungal entomopathogen Fusarium moniliforme, Botryosphaeria dothidea and both Fusarium oxysporum respectively	0.09%	18.4
Morganella morganii Species-level Match Host Order Match	RISB0008	Phormia regina Order: Diptera	deterred oviposition by female stable flies; The flies' oviposition decisions appear to be guided by bacteria-derived semiochemicals as the bacteria	0.09%	18.1
Enterococcus faecalis Species-level Match Host Order Match	RISB1411	Bactrocera dorsalis Order: Diptera	female Bactrocera dorsalis fed Enterococcus faecalis and Klebsiella oxytoca enriched diets lived longer but had lower fecundity	0.39%	17.9
Stenotrophomonas maltophilia Species-level Match Host Order Match	RISB1227	Delia antiqua Order: Diptera	six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana through symbiotic bacterium-derived organic acids	0.21%	17.9
Serratia marcescens Species-level Match Host Order Match	RISB0009	Phormia regina Order: Diptera	prompted oviposition by flies; The flies' oviposition decisions appear to be guided by bacteria-derived semiochemicals as the bacteria	0.12%	17.8
Stenotrophomonas maltophilia Species-level Match Host Order Match	RISB1141	Hermetia illucens Order: Diptera	enhance the insect growth performance when reared on an unbalanced nutritionally poor diet	0.21%	17.0
Morganella morganii Species-level Match Host Order Match	RISB0611	Bactrocera dorsalis Order: Diptera	may hydrolysing nitrogenous waste and providing metabolizable nitrogen for B. dorsalis	0.09%	16.8
Bacillus thuringiensis Species-level Match Host Order Match	RISB0820	Simulium tani Order: Diptera	show resistance to some antibiotics	0.95%	16.7
Escherichia coli Species-level Match Host Order Match	RISB1769	Calliphoridae Order: Diptera	None	1.56%	16.6
Stenotrophomonas maltophilia Species-level Match Host Order Match	RISB1401	Delia antiqua Order: Diptera	suppressed Beauveria bassiana conidia germination and hyphal growth	0.21%	16.6
Enterococcus faecalis Species-level Match Host Order Match	RISB0095	Bactrocera minax Order: Diptera	egrade phenols in unripe citrus in B. minax larvae	0.39%	16.4
Bacillus cereus Species-level Match Host Order Match	RISB1872	Aedes aegypti Order: Diptera	gut microbiome	1.05%	16.3
Lactiplantibacillus plantarum Species-level Match Host Order Match	RISB0674	Drosophila melanogaster Order: Diptera	could effectively inhibit fungal spore germinations	0.15%	16.2
Paenibacillus sp. PAMC21692 Species-level Match Host Order Match	RISB2098	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.59%	16.2
Serratia marcescens Species-level Match Host Order Match	RISB0096	Bactrocera minax Order: Diptera	egrade phenols in unripe citrus in B. minax larvae	0.12%	16.1
Lactococcus lactis Species-level Match Host Order Match	RISB1167	Bactrocera dorsalis Order: Diptera	Promote the growth of larvae	0.51%	16.1
Bacillus cereus Species-level Match Host Order Match	RISB1701	Phlebotomus papatasi Order: Diptera	None	1.05%	16.1
Streptomyces sp. SJL17-4 Species-level Match	RISB0943	Polybia plebeja Order: Hymenoptera	this bacterium produces antimicrobial compounds that are active against Hirsutella citriformis, a natural fungal enemy of its host, and the human pathogens Staphylococcus aureus and Candida albicans	6.94%	15.9
Microbacterium sp. ABRD28 Species-level Match Host Order Match	RISB2095	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.19%	15.8
Chryseobacterium sp. MYb264 Species-level Match Host Order Match	RISB2092	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.16%	15.7
Chryseobacterium sp. Chry.R1 Species-level Match Host Order Match	RISB2092	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.11%	15.7
Streptomyces sp. SJL17-4 Species-level Match	RISB2334	Sirex noctilio Order: Hymenoptera	degrading woody substrates and that such degradation may assist in nutrient acquisition by S. noctilio, thus contributing to its ability to be established in forested habitats worldwide	6.94%	15.6
Klebsiella pneumoniae Species-level Match Host Order Match	RISB1771	Muscidae Order: Diptera	None	0.59%	15.6
Buchnera aphidicola Species-level Match Host Order Match	RISB0051	Episyrphus balteatus Order: Diptera	None	0.56%	15.6
Staphylococcus hominis Species-level Match Host Order Match	RISB1881	Aedes aegypti Order: Diptera	gut microbiome	0.09%	15.4
Spiroplasma Host Order Match	RISB1796	Drosophila neotestacea Order: Diptera	when parasitized by the nematode Howardula aoronymphium, Spiroplasma encodes a ribosome-inactivating protein (RIP) related to Shiga-like toxins from enterohemorrhagic Escherichia coli and that Howardula ribosomal RNA (rRNA) is depurinated during Spiroplasma-mediated protection of D. neotestacea	0.35%	15.4
Candidatus Cardinium Host Order Match	RISB1439	Lutzomyia evansi Order: Diptera	‘Candidatus Cardinium’ is a recently described bacterium from the Bacteroidetes group involved in diverse reproduction alterations of its arthropod hosts (including cytoplasmic incompatibility, parthenogenesis, and feminization) similar to Wolbachia	0.24%	15.2
Streptomyces sp. NBC_01296 Species-level Match	RISB0943	Polybia plebeja Order: Hymenoptera	this bacterium produces antimicrobial compounds that are active against Hirsutella citriformis, a natural fungal enemy of its host, and the human pathogens Staphylococcus aureus and Candida albicans	6.23%	15.2
Lactiplantibacillus plantarum Species-level Match Host Order Match	RISB0608	Drosophila melanogaster Order: Diptera	None	0.15%	15.2
Spiroplasma Host Order Match	RISB1926	Anopheles gambiae Order: Diptera	may have reproductive interactions with their mosquito hosts，either providing an indirect fitness advantage to females by inducing male killing or by directly protecting the host against natural pathogens	0.35%	14.4
Spiroplasma Host Order Match	RISB2026	Drosophila hydei Order: Diptera	Spiroplasma protect their host against parasitoid attack. The Spiroplasma-conferred protection is partial and flies surviving a wasp attack have reduced adult longevity and fecundity	0.35%	14.0
Leucobacter Host Order Match	RISB0771	Delia antiqua Order: Diptera	showed significant contact inhibition activity against fungal entomopathogen Fusarium moniliforme, Botryosphaeria dothidea and both Fusarium oxysporum respectively	0.18%	13.4
Shewanella Host Order Match	RISB1924	Anopheles gambiae Order: Diptera	may be related with mediating adaptation to different ecological niches or in shaping specific adult behaviors including mating	0.19%	12.7
Escherichia coli Species-level Match	RISB1339	Manduca sexta Order: Lepidoptera	modulate immunity-related gene expression in the infected F0 larvae, and also in their offspring, triggered immune responses in the infected host associated with shifts in both DNA methylation and histone acetylation	1.56%	10.9
Aeromonas Host Order Match	RISB2086	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.11%	10.7
Pseudomonas sp. CIP-10 Species-level Match	RISB1622	Dendroctonus valens Order: Coleoptera	volatiles from predominant bacteria regulate the consumption sequence of carbon sources d-pinitol and d-glucose in the fungal symbiont Leptographium procerum, and appear to alleviate the antagonistic effect from the fungus against RTB larvae	0.83%	10.7
Klebsiella pneumoniae Species-level Match	RISB2185	Scirpophaga incertulas Order: Lepidoptera	The ability of these arthropods to feed on wood, foliage and detritus is likely to involve catalysis by different types of cellulases/hemicellulases that are secreted by gut microbiota to digest the structural and recalcitrant lignocellulosic residues in their foods.	0.59%	10.6
Buchnera aphidicola Species-level Match	RISB0236	Acyrthosiphon pisum Order: Hemiptera	Buchnera the nutritional endosymbiont of A. pisum is located inside of bacteriocytes and requires aspartate from the aphid host, because it cannot make it de novo. Further Buchnera needs aspartate for the biosynthesis of the essential amino acids lysine and threonine, which the aphid and Buchnera require for survival	0.56%	10.6
Peribacillus Host Order Match	RISB1877	Aedes aegypti Order: Diptera	gut microbiome	0.21%	10.5
Leucobacter Host Order Match	RISB1876	Aedes aegypti Order: Diptera	gut microbiome	0.18%	10.5
Achromobacter Host Order Match	RISB1869	Aedes aegypti Order: Diptera	gut microbiome	0.13%	10.4
Buchnera aphidicola Species-level Match	RISB2485	Macrosiphum euphorbiae Order: Hemiptera	symbiont expression patterns differ between aphid clones with differing levels of virulence, and are influenced by the aphids' host plant. Potentially, symbionts may contribute to differential adaptation of aphids to host plant resistance	0.56%	10.3
Vagococcus Host Order Match	RISB0042	Aldrichina grahami Order: Diptera	None	0.17%	10.2
Enterococcus mundtii Species-level Match	RISB1733	Spodoptera littoralis Order: Lepidoptera	actively secretes a stable class IIa bacteriocin (mundticin KS) against invading bacteria, including the opportunistic pathogens E. faecalis and E. casseliflavus, but not against other gut residents, facilitating the normal development of host gut microbiota	0.07%	10.1
Clostridium sp. AWRP Species-level Match	RISB2301	Pyrrhocoris apterus Order: Hemiptera	could play an important role for the insect by degrading complex dietary components, providing nutrient supplementation, or detoxifying noxious chemicals (e.g. cyclopropenoic fatty acids or gossypol) in the diet	0.33%	9.6
Clostridium sp. DL-VIII Species-level Match	RISB2301	Pyrrhocoris apterus Order: Hemiptera	could play an important role for the insect by degrading complex dietary components, providing nutrient supplementation, or detoxifying noxious chemicals (e.g. cyclopropenoic fatty acids or gossypol) in the diet	0.08%	9.3
Escherichia coli Species-level Match	RISB0128	Tribolium castaneum Order: Coleoptera	may produce 4,8-dimethyldecanal (DMD) production that is strongly associated with attraction to females and host pheromone communication	1.56%	9.3
Clostridium sp. OS1-26 Species-level Match	RISB2301	Pyrrhocoris apterus Order: Hemiptera	could play an important role for the insect by degrading complex dietary components, providing nutrient supplementation, or detoxifying noxious chemicals (e.g. cyclopropenoic fatty acids or gossypol) in the diet	0.06%	9.3
Pseudomonas sp. CIP-10 Species-level Match	RISB2224	Leptinotarsa decemlineata Order: Coleoptera	Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)	0.83%	9.2
Mammaliicoccus sciuri Species-level Match	RISB0075	Bombyx mori Order: Lepidoptera	could produce a secreted chitinolytic lysozyme (termed Msp1) to damage fungal cell walls，completely inhibit the spore germination of fungal entomopathogens Metarhizium robertsii and Beauveria bassiana	0.15%	9.2
Acinetobacter pittii Species-level Match	RISB1977	Blattella germanica Order: Blattodea	gut microbiota contributes to production of VCAs that act as fecal aggregation agents and that cockroaches discriminate among the complex odors that emanate from a diverse microbial community	0.30%	9.1
Sphingobacterium sp. ML3W Species-level Match	RISB2227	Leptinotarsa decemlineata Order: Coleoptera	Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)	0.17%	8.5
Blattabacterium cuenoti Species-level Match	RISB0133	Panesthiinae Order: Blattodea	enables hosts to subsist on a nutrient-poor diet; endosymbiont genome erosions are associated with repeated host transitions to an underground life	0.44%	8.4
Proteus vulgaris Species-level Match	RISB0001	Leptinotarsa decemlineata Order: Coleoptera	produces toxic hydrogen cyanide (HCN) and a mandelonitrile-producing cyanoglucoside, amygdalin, which protect the insect from predation	0.14%	7.8
Pseudomonas sp. CIP-10 Species-level Match	RISB0700	Nilaparvata lugens Order: Hemiptera	Pseudomonas sp. composition and abundance correlated with BPH survivability	0.83%	7.3
Klebsiella pneumoniae Species-level Match	RISB2459	Bombyx mori Order: Lepidoptera	degradation of cellulose, xylan, pectin and starch	0.59%	6.6
Blattabacterium cuenoti Species-level Match	RISB0518	Cryptocercus punctulatus Order: Blattodea	collaborative arginine biosynthesis	0.44%	6.1
Proteus vulgaris Species-level Match	RISB2460	Bombyx mori Order: Lepidoptera	degradation of cellulose, xylan, pectin and starch	0.14%	6.1
Salmonella enterica Species-level Match	RISB0413	Melanaphis sacchari Order: Hemiptera	None	0.93%	5.9
Blattabacterium cuenoti Species-level Match	RISB0093	Blattella germanica Order: Blattodea	obligate endosymbiont	0.44%	5.9
Flavobacterium johnsoniae Species-level Match	RISB0659	Melanaphis bambusae Order: Hemiptera	None	0.54%	5.5
Staphylococcus epidermidis Species-level Match	RISB1070	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.14%	5.4
Staphylococcus hominis Species-level Match	RISB1071	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.09%	5.3
Candidatus Cardinium	RISB0223	Bemisia tabaci Order: Hemiptera	Cardinium could inhibit the defense response of the host plant and decrease the detoxification metabolism ability of the host whitefly, decrease the expression of detoxification metabolism genes, especially the uridine 5'-diphospho-glucuronyltransferase and P450 genes,	0.24%	5.2
Gilliamella	RISB0102	Apis mellifera Order: Hymenoptera	Gilliamella apicola carries the gene for the desaturase FADS2, which is able to metabolize polyunsaturated fatty acids from pollen and synthesize endocannabinoid, a lipogenic neuroactive substance, thereby modulating reward learning and memory in honeybees.	0.11%	5.1
Candidatus Erwinia haradaeae Species-level Match	RISB1632	Lachninae Order: Hemiptera	None	0.10%	5.1
Francisella	RISB1907	Bombyx mori Order: Lepidoptera	After infection with F. tularensis, the induction of melanization and nodulation, which are immune responses to bacterial infection, were inhibited in silkworms. Pre-inoculation of silkworms with F. tularensis enhanced the expression of antimicrobial peptides and resistance to infection by pathogenic bacteria.	0.10%	5.1
Treponema	RISB2377	termite Order: Blattodea	when grown together, two termite-gut Treponema species influence each other's gene expression in a far more comprehensive and nuanced manner than might have been predicted based on the results of previous studies on the respective pure cultures	0.13%	5.0
Xanthomonas	RISB0498	Xylocopa appendiculata Order: Hymenoptera	Xanthomonas strain from Japanese carpenter bee is effective PU-degradable bacterium and is able to use polyacryl-based PU as a nutritional source, as well as other types of PS-PU and PE-PU	0.24%	4.0
Rhizobium	RISB0135	Coccinella septempunctata Order: Coleoptera	be commonly found in plant roots and they all have nitrogen fixation abilities	1.37%	2.9
Streptococcus	RISB2625	Galleria mellonella Order: Lepidoptera	suppress bacteria ingested with food by producing bacteriocin and by releasing a lysozyme like enzyme	0.89%	2.9
Bacteroides	RISB0256	Leptocybe invasa Order: Hymenoptera	Differences in Male-Killing Rickettsia Bacteria between Lineages of the Invasive Gall-Causing Pest Leptocybe invasa	0.45%	2.8
Bacteroides	RISB0090	Hyphantria cunea Order: Lepidoptera	enhance the compatibility of invasive pests to new hosts and enable more rapid adaptation to new habitats.	0.45%	2.6
Streptococcus	RISB2624	Reticulitermes flavipes Order: Blattodea	can be broken down into substances such as carbon dioxide, ammonia and acetic acid	0.89%	2.5
Bacteroides	RISB1183	Oryzaephilus surinamensis Order: Coleoptera	supplement precursors for the cuticle synthesis and thereby enhance desiccation resistance of its host	0.45%	2.5
Candidatus Cardinium	RISB2290	Sogatella furcifera Order: Hemiptera	dual infection with Cardinium and Wolbachia induced strong cytoplasmic incompatibility (CI) in a single host	0.24%	2.4
Xanthomonas	RISB0217	Xylocopa appendiculata Order: Hymenoptera	strains biodegraded polyethylene terephthalate PET powder, broke it into its degradation products	0.24%	2.2
Streptococcus	RISB2604	Homona magnanima Order: Lepidoptera	influence the growth of Bacillus thuringiensis in the larvae	0.89%	2.1
Bradyrhizobium	RISB0135	Coccinella septempunctata Order: Coleoptera	be commonly found in plant roots and they all have nitrogen fixation abilities	0.16%	1.7
Halomonas	RISB1808	Monochamus galloprovincialis Order: Coleoptera	Have the ability for degradation of cellulose, proteins and starch	0.21%	1.5
Priestia	RISB0839	Helicoverpa armigera Order: Lepidoptera	producing amylase	1.03%	1.4
Paraclostridium	RISB0028	Sesamia inferens Order: Lepidoptera	degrade Chlorpyrifos and Chlorantraniliprole in vitro	0.23%	1.3
Curtobacterium	RISB1910	Hyles euphorbiae Order: Lepidoptera	able to degrade alkaloids and/or latex	0.37%	1.1
Aeromonas	RISB2456	Bombyx mori Order: Lepidoptera	able to utilize the CMcellulose and xylan	0.11%	0.9
Mycobacterium	RISB1156	Nicrophorus concolor Order: Coleoptera	produces Antimicrobial compounds	0.15%	0.8
Aeromonas	RISB1145	Tenebrio molitor Order: Coleoptera	degrading plastics	0.11%	0.5
Gilliamella	RISB0620	Spodoptera frugiperda Order: Lepidoptera	degrade amygdalin	0.11%	0.5
Metabacillus	RISB0902	Myzus persicae Order: Hemiptera	None	0.40%	0.4
Curtobacterium	RISB0900	Myzus persicae Order: Hemiptera	None	0.37%	0.4
Cupriavidus	RISB0694	Alydus tomentosus Order: Hemiptera	None	0.33%	0.3
Helicobacter	RISB0662	Melanaphis bambusae Order: Hemiptera	None	0.25%	0.3
Halomonas	RISB1374	Bemisia tabaci Order: Hemiptera	None	0.21%	0.2
Achromobacter	RISB0383	Aphis gossypii Order: Hemiptera	None	0.13%	0.1
Treponema	RISB0169	Reticulitermes flaviceps Order: Blattodea	None	0.13%	0.1
Gilliamella	RISB1945	Apis cerana Order: Hymenoptera	None	0.11%	0.1
Legionella	RISB1687	Polyplax serrata Order: Phthiraptera	None	0.11%	0.1