SRR19201354 - Dinoderus minutus

Basic Information

Run: SRR19201354

Assay Type: WGS

Bioproject: PRJNA836854

Biosample: SAMN28175365

Bytes: 3362353794

Center Name: JOHANNES GUTENBERG-UNIVERSITY MAINZ

Sequencing Information

Instrument: Illumina HiSeq 3000

Library Layout: PAIRED

Library Selection: RANDOM

Platform: ILLUMINA

Quality Control Information

Filter Percentage: -

QC Average Length: -

Retained Reads: -

Geographic Information

Country: Japan

Continent: Asia

Location Name: Japan: ita

Latitude/Longitude: -

Sample Information

Host: Dinoderus minutus

Isolation: beetle abdomen

Biosample Model: Metagenome or environmental

Collection Date: 2016-04-12

Taxonomic Classification

Potential Symbionts

About Potential Symbionts

This table shows potential symbiont identified in the metagenome sample. Matches are scored based on:

Relative abundance in the sample
Species-level matches with known symbionts
Host insect order matches with reference records
Completeness and richness of functional records

Note: only the three highest scoring comparison records were retained for each symbiont species or genus in the sample

Based on our current records database, this section aims to identify potential functional symbionts in this metagenome sample, with scoring based on:

Relative abundance in sample
Species-level matches with known symbionts
Host insect order matches
Functional record completeness

Note: Showing top 3 highest scoring records for each species/genus

Symbiont Name	Record	Host Species	Function	Abundance	Score Score Composition: Symbiont Abundance Species match bonus Host match bonus Function richness Higher scores indicate stronger symbiotic relationship potential
Blattabacterium cuenoti Species-level Match	RISB0133	Panesthiinae Order: Blattodea	enables hosts to subsist on a nutrient-poor diet; endosymbiont genome erosions are associated with repeated host transitions to an underground life	16.63%	24.6
Blattabacterium cuenoti Species-level Match	RISB0518	Cryptocercus punctulatus Order: Blattodea	collaborative arginine biosynthesis	16.63%	22.3
Blattabacterium cuenoti Species-level Match	RISB0093	Blattella germanica Order: Blattodea	obligate endosymbiont	16.63%	22.1
Pseudomonas sp. CIP-10 Species-level Match Host Order Match	RISB1622	Dendroctonus valens Order: Coleoptera	volatiles from predominant bacteria regulate the consumption sequence of carbon sources d-pinitol and d-glucose in the fungal symbiont Leptographium procerum, and appear to alleviate the antagonistic effect from the fungus against RTB larvae	0.47%	20.3
Stenotrophomonas sp. 610A2 Species-level Match Host Order Match	RISB0325	Pharaxonotha floridana Order: Coleoptera	suggesting the occurrence of an unprecedented desferrioxamine-like biosynthetic pathway,including desferrioxamine B, which may help tolerating diets rich in azoxyglycosides, BMAA, and other cycad toxins, including a possible role for bacterial siderophores	0.06%	20.1
Stenotrophomonas sp. SAU14A_NAIMI4_5 Species-level Match Host Order Match	RISB0325	Pharaxonotha floridana Order: Coleoptera	suggesting the occurrence of an unprecedented desferrioxamine-like biosynthetic pathway,including desferrioxamine B, which may help tolerating diets rich in azoxyglycosides, BMAA, and other cycad toxins, including a possible role for bacterial siderophores	0.03%	20.0
Pantoea sp. SS70 Species-level Match Host Order Match	RISB0736	Psylliodes chrysocephala Order: Coleoptera	Laboratory-reared and field-collected P. chrysocephala all contained three core genera Pantoea, Acinetobacter and Pseudomonas, and reintroduction of Pantoea sp. Pc8 in antibiotic-fed beetles restored isothiocyanate degradation ability in vivo (by 16S rRNA gene sequencing and LC-MS)	0.01%	20.0
Burkholderia gladioli Species-level Match Host Order Match	RISB1172	Lagria villosa Order: Coleoptera	process a cryptic gene cluster that codes for the biosynthesis of a novel antifungal polyketide with a glutarimide pharmacophore, which led to the discovery of the gladiofungins as previously-overlooked components of the antimicrobial armory of the beetle symbiont	0.01%	20.0
Staphylococcus gallinarum Species-level Match Host Order Match	RISB0945	Callosobruchus maculatus Order: Coleoptera	The strain encodes complete biosynthetic pathways for the production of B vitamins and amino acids, including tyrosine; A carbohydrate-active enzyme search revealed that the genome codes for a number of digestive enzymes, reflecting the nutritional ecology of C. maculatus	0.01%	20.0
Pseudomonas sp. FP198 Species-level Match Host Order Match	RISB1622	Dendroctonus valens Order: Coleoptera	volatiles from predominant bacteria regulate the consumption sequence of carbon sources d-pinitol and d-glucose in the fungal symbiont Leptographium procerum, and appear to alleviate the antagonistic effect from the fungus against RTB larvae	0.03%	19.9
Pseudomonas sp. IT1137 Species-level Match Host Order Match	RISB1622	Dendroctonus valens Order: Coleoptera	volatiles from predominant bacteria regulate the consumption sequence of carbon sources d-pinitol and d-glucose in the fungal symbiont Leptographium procerum, and appear to alleviate the antagonistic effect from the fungus against RTB larvae	0.02%	19.8
Escherichia coli Species-level Match Host Order Match	RISB0128	Tribolium castaneum Order: Coleoptera	may produce 4,8-dimethyldecanal (DMD) production that is strongly associated with attraction to females and host pheromone communication	2.00%	19.7
Acinetobacter sp. A1-4-2 Species-level Match Host Order Match	RISB0730	Curculio chinensis Order: Coleoptera	Acinetobacter sp. in C. chinensis enriched after treating with saponin, and when incubating bacteria with saponin for 72 h, saponin content significantly decreased from 4.054 to 1.867 mg/mL (by 16S rRNA metagenome sequencing and HPLC)	0.04%	19.7
Acinetobacter sp. XS-4 Species-level Match Host Order Match	RISB0730	Curculio chinensis Order: Coleoptera	Acinetobacter sp. in C. chinensis enriched after treating with saponin, and when incubating bacteria with saponin for 72 h, saponin content significantly decreased from 4.054 to 1.867 mg/mL (by 16S rRNA metagenome sequencing and HPLC)	0.01%	19.7
Burkholderia gladioli Species-level Match Host Order Match	RISB1729	Lagria hirta Order: Coleoptera	the symbionts inhibit the growth of antagonistic fungi on the eggs of the insect host, indicating that the Lagria-associated Burkholderia have evolved from plant pathogenic ancestors into insect defensive mutualists	0.01%	19.3
Burkholderia gladioli Species-level Match Host Order Match	RISB1604	Lagria villosa Order: Coleoptera	Bacteria produce icosalide, an unusual two-tailed lipocyclopeptide antibiotic,which is active against entomopathogenic bacteria, thus adding to the chemical armory protecting beetle offspring	0.01%	18.8
Lactococcus lactis Species-level Match Host Order Match	RISB0967	Oulema melanopus Order: Coleoptera	contribute to the decomposition of complex carbohydrates, fatty acids, or polysaccharides in the insect gut. It might also contribute to the improvement of nutrient availability.	0.18%	18.7
Sphingobacterium sp. ML3W Species-level Match Host Order Match	RISB2227	Leptinotarsa decemlineata Order: Coleoptera	Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)	0.08%	18.4
Stenotrophomonas sp. 610A2 Species-level Match Host Order Match	RISB2228	Leptinotarsa decemlineata Order: Coleoptera	Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)	0.06%	18.4
Sphingobacterium sp. WM Species-level Match Host Order Match	RISB2227	Leptinotarsa decemlineata Order: Coleoptera	Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)	0.06%	18.4
Sphingobacterium sp. UDSM-2020 Species-level Match Host Order Match	RISB2227	Leptinotarsa decemlineata Order: Coleoptera	Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)	0.02%	18.4
Klebsiella oxytoca Species-level Match Host Order Match	RISB1506	Cleonus trivittatus Order: Coleoptera	Antibiotic-treated larvae suffered growth retardation on a diet containing plant extract or swainsonine. Gut bacteria showed toxin-degradation activities in vitro	0.01%	18.3
Enterococcus faecalis Species-level Match Host Order Match	RISB0497	Cryptolestes ferrugineus Order: Coleoptera	bacteria can degrade malathion, pirimiphos-methyl, and deltamethrin and utilize these insecticides as the carbon source in vitro.	0.53%	18.1
Citrobacter freundii Species-level Match Host Order Match	RISB0517	Leptinotarsa decemlineata Order: Coleoptera	affect the cellular and humoral immunity of the insect, increasing its susceptibility to Bacillus thuringiensis var. tenebrionis (morrisoni) (Bt)	0.07%	18.0
Enterococcus faecalis Species-level Match Host Order Match	RISB2042	Harpalus pensylvanicus Order: Coleoptera	E. faecalis facilitate seed consumption by H. pensylvanicus, possibly by contributing digestive enzymes to their host	0.53%	17.9
Morganella morganii Species-level Match Host Order Match	RISB1867	Costelytra zealandica Order: Coleoptera	Female beetles were previously shown to use phenol as their sex pheromone produced by symbiotic bacteria in the accessory or colleterial gland	0.03%	17.9
Bacillus sp. FDAARGOS_235 Species-level Match Host Order Match	RISB1645	Osphranteria coerulescens Order: Coleoptera	The isolate has cellulolytic activity and can hydrolyze CMC, avicel, cellulose and sawdust with broad temperature and pH stability	0.21%	17.8
Citrobacter freundii Species-level Match Host Order Match	RISB0127	Tribolium castaneum Order: Coleoptera	may produce 4,8-dimethyldecanal (DMD) production that is strongly associated with attraction to females and host pheromone communication	0.07%	17.8
Bacillus subtilis Species-level Match Host Order Match	RISB0494	Sitophilus oryzae Order: Coleoptera	bacteria can degrade malathion, pirimiphos-methyl, and deltamethrin and utilize these insecticides as the carbon source in vitro.	0.17%	17.8
Proteus vulgaris Species-level Match Host Order Match	RISB0001	Leptinotarsa decemlineata Order: Coleoptera	produces toxic hydrogen cyanide (HCN) and a mandelonitrile-producing cyanoglucoside, amygdalin, which protect the insect from predation	0.05%	17.8
Bacillus sp. T3 Species-level Match Host Order Match	RISB1645	Osphranteria coerulescens Order: Coleoptera	The isolate has cellulolytic activity and can hydrolyze CMC, avicel, cellulose and sawdust with broad temperature and pH stability	0.04%	17.6
Staphylococcus gallinarum Species-level Match Host Order Match	RISB0946	Callosobruchus maculatus Order: Coleoptera	The strain encodes complete biosynthetic pathways for the production of B vitamins and amino acids, including tyrosine	0.01%	17.4
Acinetobacter sp. A1-4-2 Species-level Match Host Order Match	RISB0706	Curculio chinensis Order: Coleoptera	facilitate the degradation of tea saponin; genome contains 47 genes relating to triterpenoids degradation	0.04%	17.1
Serratia marcescens Species-level Match Host Order Match	RISB1295	Nicrophorus vespilloides Order: Coleoptera	producing antibacterial compound Serrawettin W2, which has antibacterial and nematode-inhibiting effects	0.06%	17.1
Lactococcus lactis Species-level Match Host Order Match	RISB1430	Rhynchophorus ferrugineus Order: Coleoptera	promote the development and body mass gain of RPW larvae by improving their nutrition metabolism	0.18%	17.1
Klebsiella pneumoniae Species-level Match Host Order Match	RISB1153	Tenebrio molitor Order: Coleoptera	degrading plastics	1.74%	17.1
Enterococcus faecalis Species-level Match Host Order Match	RISB0374	Tribolium castaneum Order: Coleoptera	modulates host phosphine resistance by interfering with the redox system	0.53%	17.0
Enterobacter cloacae complex sp. Species-level Match Host Order Match	RISB1428	Rhynchophorus ferrugineus Order: Coleoptera	promote the development and body mass gain of RPW larvae by improving their nutrition metabolism	0.03%	17.0
Enterobacter cloacae Species-level Match Host Order Match	RISB1428	Rhynchophorus ferrugineus Order: Coleoptera	promote the development and body mass gain of RPW larvae by improving their nutrition metabolism	0.01%	16.9
Paludibacter propionicigenes Species-level Match Host Order Match	RISB2055	Odontotaenius disjunctus Order: Coleoptera	microbial fixation of nitrogen that is important for this beetle to subsist on woody biomass	0.01%	16.9
Streptomyces sp. T12 Species-level Match Host Order Match	RISB0777	Copris tripartitus Order: Coleoptera	contribute brood ball hygiene by inhibiting fungal parasites in the environment	0.27%	16.9
Serratia marcescens Species-level Match Host Order Match	RISB0365	Pagiophloeus tsushimanus Order: Coleoptera	terpenoid-degrading: the highest degradation rates of D-camphor, linalool, and eucalyptol	0.06%	16.8
Morganella morganii Species-level Match Host Order Match	RISB1548	Costelytra zealandica Order: Coleoptera	symbionts residing in the colleterial glands produce phenol 1 as the female sex pheromone	0.03%	16.8
Morganella morganii Species-level Match Host Order Match	RISB1868	Costelytra zealandica Order: Coleoptera	produces phenol as the sex pheromone of the host from tyrosine in the colleterial gland	0.03%	16.8
Streptomyces sp. NBC_00354 Species-level Match Host Order Match	RISB0777	Copris tripartitus Order: Coleoptera	contribute brood ball hygiene by inhibiting fungal parasites in the environment	0.10%	16.7
Streptomyces sp. BPPL-273 Species-level Match Host Order Match	RISB0777	Copris tripartitus Order: Coleoptera	contribute brood ball hygiene by inhibiting fungal parasites in the environment	0.08%	16.7
Paenibacillus sp. FSL R10-2796 Species-level Match Host Order Match	RISB0813	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-9 oxidation pathway	0.07%	16.5
Pantoea sp. SS70 Species-level Match Host Order Match	RISB0814	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-14 oxidation pathway	0.01%	16.4
Paenibacillus sp. FSL W8-0194 Species-level Match Host Order Match	RISB0813	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-9 oxidation pathway	0.02%	16.4
Paenibacillus sp. BIC5C1 Species-level Match Host Order Match	RISB0813	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-9 oxidation pathway	0.01%	16.4
Serratia marcescens Species-level Match Host Order Match	RISB1158	Nicrophorus vespilloides Order: Coleoptera	produces an antibacterial cyclic lipopeptide called serrawettin W2	0.06%	16.4
Paludibacter propionicigenes Species-level Match Host Order Match	RISB2056	Odontotaenius disjunctus Order: Coleoptera	plays an important role in nitrogen fixation	0.01%	15.9
Enterobacter asburiae Species-level Match Host Order Match	RISB1150	Plodia interpunctella Order: Coleoptera	damaged polyethylene (PE) films	0.03%	15.7
Lactococcus lactis Species-level Match Host Order Match	RISB1065	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.18%	15.4
Staphylococcus epidermidis Species-level Match Host Order Match	RISB1070	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.12%	15.3
Lysinibacillus fusiformis Species-level Match Host Order Match	RISB1066	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.05%	15.3
Pantoea agglomerans Species-level Match Host Order Match	RISB1858	Lissorhoptrus oryzophilus Order: Coleoptera	None	0.02%	15.0
Sphingobium Host Order Match	RISB1837	Dendroctonus valens Order: Coleoptera	It can trongly degrade naringenin, and pinitol, the main soluble carbohydrate of P. tabuliformis, is retained in L. procerum-infected phloem and facilitate naringenin biodegradation by the microbiotas.	0.02%	14.0
Novosphingobium Host Order Match	RISB1837	Dendroctonus valens Order: Coleoptera	It can trongly degrade naringenin, and pinitol, the main soluble carbohydrate of P. tabuliformis, is retained in L. procerum-infected phloem and facilitate naringenin biodegradation by the microbiotas.	0.02%	14.0
Wolbachia Host Order Match	RISB1452	Octodonta nipae Order: Coleoptera	Wolbachia harbored dominantly in a female than the male adult, while, no significant differences were observed between male and female body parts and tissues	0.85%	14.0
Raoultella Host Order Match	RISB2226	Leptinotarsa decemlineata Order: Coleoptera	Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)	0.02%	13.4
Wolbachia Host Order Match	RISB2107	Sitophilus zeamais Order: Coleoptera	Wolbachia directly favored weevil fertility and exhibited only mild indirect effects, usually enhancing the SZPE effect	0.85%	13.2
Vibrio Host Order Match	RISB1810	Monochamus galloprovincialis Order: Coleoptera	Have the ability for degradation of cellulose, proteins and starch	1.37%	12.7
Bacteroides Host Order Match	RISB1183	Oryzaephilus surinamensis Order: Coleoptera	supplement precursors for the cuticle synthesis and thereby enhance desiccation resistance of its host	0.58%	12.6
Wolbachia Host Order Match	RISB1282	Ips sp. Order: Coleoptera	inducing cytoplasmic incompatibility, resulting in reproductive distortions and hence	0.85%	12.6
Rickettsia Host Order Match	RISB1279	Ips sp. Order: Coleoptera	inducing cytoplasmic incompatibility, resulting in reproductive distortions and hence	0.12%	11.8
Klebsiella pneumoniae Species-level Match	RISB2185	Scirpophaga incertulas Order: Lepidoptera	The ability of these arthropods to feed on wood, foliage and detritus is likely to involve catalysis by different types of cellulases/hemicellulases that are secreted by gut microbiota to digest the structural and recalcitrant lignocellulosic residues in their foods.	1.74%	11.7
Rickettsia Host Order Match	RISB0970	Oulema melanopus Order: Coleoptera	may be associated with insect reproduction and maturation of their sexual organs	0.12%	11.7
Rickettsia Host Order Match	RISB1954	Sitona obsoletus Order: Coleoptera	potential defensive properties against he parasitoid Microctonus aethiopoides	0.12%	11.7
Rhizobium Host Order Match	RISB0135	Coccinella septempunctata Order: Coleoptera	be commonly found in plant roots and they all have nitrogen fixation abilities	0.09%	11.7
Nostoc Host Order Match	RISB0812	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-18 oxidation pathway	0.22%	11.6
Candidatus Nardonella Host Order Match	RISB2449	Euscepes postfasciatus Order: Coleoptera	endosymbiont is involved in normal growth and development of the host weevil	0.02%	11.5
Halomonas Host Order Match	RISB1808	Monochamus galloprovincialis Order: Coleoptera	Have the ability for degradation of cellulose, proteins and starch	0.18%	11.5
Candidatus Nardonella Host Order Match	RISB1931	Lissorhoptrus oryzophilus Order: Coleoptera	might be not playing critical roles in the reproduction of L. oryzophilus	0.02%	11.5
Delftia Host Order Match	RISB0806	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-19 oxidation pathway	0.06%	11.5
Leuconostoc Host Order Match	RISB0812	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-18 oxidation pathway	0.04%	11.5
Kosakonia Host Order Match	RISB0810	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-16 oxidation pathway	0.03%	11.5
Candidatus Mesenet Host Order Match	RISB1785	Brontispa longissima Order: Coleoptera	induced complete Cytoplasmic incompatibility (CI) (100% mortality)	0.01%	11.3
Escherichia coli Species-level Match	RISB1339	Manduca sexta Order: Lepidoptera	modulate immunity-related gene expression in the infected F0 larvae, and also in their offspring, triggered immune responses in the infected host associated with shifts in both DNA methylation and histone acetylation	2.00%	11.3
Raoultella Host Order Match	RISB1007	Monochamus alternatus Order: Coleoptera	may help M. alternatus degrade cellulose and pinene	0.02%	11.0
Buchnera aphidicola Species-level Match	RISB0236	Acyrthosiphon pisum Order: Hemiptera	Buchnera the nutritional endosymbiont of A. pisum is located inside of bacteriocytes and requires aspartate from the aphid host, because it cannot make it de novo. Further Buchnera needs aspartate for the biosynthesis of the essential amino acids lysine and threonine, which the aphid and Buchnera require for survival	0.91%	10.9
Candidatus Nardonella Host Order Match	RISB1668	Multiple species Order: Coleoptera	Possibly tyrosine precursor provisioning	0.02%	10.8
Mycobacterium Host Order Match	RISB1156	Nicrophorus concolor Order: Coleoptera	produces Antimicrobial compounds	0.11%	10.8
Buchnera aphidicola Species-level Match	RISB2485	Macrosiphum euphorbiae Order: Hemiptera	symbiont expression patterns differ between aphid clones with differing levels of virulence, and are influenced by the aphids' host plant. Potentially, symbionts may contribute to differential adaptation of aphids to host plant resistance	0.91%	10.7
Turicibacter Host Order Match	RISB0451	Odontotaenius disjunctus Order: Coleoptera	degrading ellulose and xylan	0.03%	10.6
Aeromonas Host Order Match	RISB1145	Tenebrio molitor Order: Coleoptera	degrading plastics	0.09%	10.5
Kosakonia Host Order Match	RISB1155	Tenebrio molitor Order: Coleoptera	degrading plastics	0.03%	10.4
Candidatus Walczuchella monophlebidarum Species-level Match	RISB2075	Llaveia axin axin Order: Hemiptera	could be supplying most of these precursors for the amino acid biosynthesis as it has the potential to make ribulose-5P from ribose-1P and also PEP and pyruvate from glycolysis. It is also capable of producing homocysteine from homoserine for methionine biosynthesis,	0.12%	10.1
Dysgonomonas Host Order Match	RISB1481	Brachinus elongatulus Order: Coleoptera	None	0.04%	10.0
Listeria monocytogenes Species-level Match	RISB2308	Drosophila melanogaster Order: Diptera	L. monocytogenes infection disrupts host energy metabolism by depleting energy stores (triglycerides and glycogen) and reducing metabolic pathway activity (beta-oxidation and glycolysis). The infection affects antioxidant defense by reducing uric acid levels and alters amino acid metabolism. These metabolic changes are accompanied by melanization, potentially linked to decreased tyrosine levels.	0.03%	10.0
Francisella tularensis Species-level Match	RISB1907	Bombyx mori Order: Lepidoptera	After infection with F. tularensis, the induction of melanization and nodulation, which are immune responses to bacterial infection, were inhibited in silkworms. Pre-inoculation of silkworms with F. tularensis enhanced the expression of antimicrobial peptides and resistance to infection by pathogenic bacteria.	0.02%	10.0
Gilliamella apicola Species-level Match	RISB0102	Apis mellifera Order: Hymenoptera	Gilliamella apicola carries the gene for the desaturase FADS2, which is able to metabolize polyunsaturated fatty acids from pollen and synthesize endocannabinoid, a lipogenic neuroactive substance, thereby modulating reward learning and memory in honeybees.	0.01%	10.0
Buchnera aphidicola Species-level Match	RISB0685	Acyrthosiphon pisum Order: Hemiptera	It supplies the host with vitamins and essential amino acids, such as arginine and methionine that aphids cannot synthesize or derive insufficiently from their diet, the phloem sap of plants	0.91%	9.7
Candidatus Nasuia deltocephalinicola Species-level Match	RISB2283	Nephotettix cincticeps Order: Hemiptera	Oral administration of tetracycline to nymphal N. cincticeps resulted in retarded growth, high mortality rates, and failure in adult emergence, suggesting important biological roles of the symbionts for the host insect	0.01%	9.4
Clostridium sp. JN-9 Species-level Match	RISB2301	Pyrrhocoris apterus Order: Hemiptera	could play an important role for the insect by degrading complex dietary components, providing nutrient supplementation, or detoxifying noxious chemicals (e.g. cyclopropenoic fatty acids or gossypol) in the diet	0.11%	9.3
Clostridium sp. OS1-26 Species-level Match	RISB2301	Pyrrhocoris apterus Order: Hemiptera	could play an important role for the insect by degrading complex dietary components, providing nutrient supplementation, or detoxifying noxious chemicals (e.g. cyclopropenoic fatty acids or gossypol) in the diet	0.06%	9.3
Clostridium sp. MB40-C1 Species-level Match	RISB2301	Pyrrhocoris apterus Order: Hemiptera	could play an important role for the insect by degrading complex dietary components, providing nutrient supplementation, or detoxifying noxious chemicals (e.g. cyclopropenoic fatty acids or gossypol) in the diet	0.05%	9.3
Mammaliicoccus sciuri Species-level Match	RISB0075	Bombyx mori Order: Lepidoptera	could produce a secreted chitinolytic lysozyme (termed Msp1) to damage fungal cell walls，completely inhibit the spore germination of fungal entomopathogens Metarhizium robertsii and Beauveria bassiana	0.06%	9.1
Candidatus Carsonella ruddii Species-level Match	RISB0394	Cacopsylla pyricola Order: Hemiptera	Carsonella produces most essential amino acids (EAAs) for C. pyricola, Psyllophila complements the genes missing in Carsonella for the tryptophan pathway and synthesizes some vitamins and carotenoids	0.02%	9.0
Citrobacter sp. CRE-46 Species-level Match	RISB1503	Bactrocera dorsalis Order: Diptera	Pesticide-degrading bacteria were frequently detected from pesticide-resistant insects. Susceptible insects became resistant after inoculation of the pesticide-degrading symbiont	0.10%	8.7
Candidatus Portiera aleyrodidarum Species-level Match	RISB1193	Bemisia tabaci Order: Hemiptera	synthesizing essential amino acid (e.g. tryptophan, leucine and L-Isoleucine), Bemisia tabaci provides vital nutritional support for growth, development and reproduction	0.03%	8.4
Lactobacillus sp. PV034 Species-level Match	RISB0292	Lymantria dispar asiatica Order: Lepidoptera	Beauveria bassiana infection-based assays showed that the mortality of non-axenic L. dispar asiatica larvae was significantly higher than that of axenic larvae at 72 h.	0.04%	8.4
Spiroplasma sp. SV19 Species-level Match	RISB1353	Cephus cinctus Order: Hymenoptera	The bacterium also encoded biosynthetic pathways for essential vitamins B2, B3, and B9. We identified putative Spiroplasma virulence genes: cardiolipin and chitinase.	0.03%	8.4
Spiroplasma sp. TIUS-1 Species-level Match	RISB1353	Cephus cinctus Order: Hymenoptera	The bacterium also encoded biosynthetic pathways for essential vitamins B2, B3, and B9. We identified putative Spiroplasma virulence genes: cardiolipin and chitinase.	0.02%	8.3
Wigglesworthia glossinidia Species-level Match	RISB0369	Glossina morsitans Order: Diptera	symbiont-derived factors, likely B vitamins, are critical for the proper function of both lipid biosynthesis and lipolysis to maintain tsetse fly fecundity	0.07%	8.2
Escherichia coli Species-level Match	RISB2120	Galleria mellonella Order: Lepidoptera	mediate trans-generational immune priming	2.00%	7.8
Spiroplasma ixodetis Species-level Match	RISB0842	Dactylopius coccus Order: Hemiptera	use the T4SS to interact with the Dactylopius cells, which show a strong interaction and molecular signaling in the symbiosis	0.11%	7.6
Comamonas terrigena Species-level Match	RISB2021	Bactrocera dorsalis Order: Diptera	This group in the immature stages may be helping the insects to cope with oxidative stress by supplementing available oxygen.	0.04%	7.5
Carnobacterium maltaromaticum Species-level Match	RISB1693	Plutella xylostella Order: Lepidoptera	play an important role in the breakdown of plant cell walls, detoxification of plant phenolics, and synthesis of amino acids.	0.03%	7.5
Candidatus Nasuia deltocephalinicola Species-level Match	RISB2282	Nephotettix cincticeps Order: Hemiptera	With the antibiotic, nymphal growth was remarkably retarded, and a number of nymphs either died or failed to attain adulthood	0.01%	7.5
Psychrobacter sp. 28M-43 Species-level Match	RISB1773	Calliphoridae Order: Diptera	it shows physiological adaptation to survival in warmer temperatures and has been previously associated with food spoilage	0.04%	7.5
Psychrobacter sp. M13 Species-level Match	RISB1773	Calliphoridae Order: Diptera	it shows physiological adaptation to survival in warmer temperatures and has been previously associated with food spoilage	0.01%	7.5
Candidatus Nasuia deltocephalinicola Species-level Match	RISB0262	Maiestas dorsalis Order: Hemiptera	are responsible for synthesizing two essential amino acids (histidine and methionine) and riboflavin (vitamin B2)	0.01%	7.3
Salmonella enterica Species-level Match	RISB0413	Melanaphis sacchari Order: Hemiptera	None	2.26%	7.3
Candidatus Portiera aleyrodidarum Species-level Match	RISB2289	Bemisia tabaci Order: Hemiptera	encoding the capability to synthetize, or participate in the synthesis of, several amino acids and carotenoids,	0.03%	7.3
Wigglesworthia glossinidia Species-level Match	RISB1786	Glossina morsitans Order: Diptera	Synthesis of a large number of B vitamins, to supplement the host nutritional deficiencies of the diet	0.07%	7.1
Apilactobacillus kunkeei Species-level Match	RISB0475	Apis mellifera Order: Hymenoptera	A. kunkeei alleviated acetamiprid-induced symbiotic microbiota dysregulation and mortality in honeybees	0.01%	7.1
Candidatus Portiera aleyrodidarum Species-level Match	RISB1973	Bemisia tabaci Order: Hemiptera	a primary symbiont, which compensates for the deficient nutritional composition of its food sources	0.03%	7.0
Snodgrassella alvi Species-level Match	RISB1423	Bombus spp. Order: Hymenoptera	The bumble bee microbiome slightly increases survivorship when the host is exposed to selenate	0.01%	6.9
Leclercia adecarboxylata Species-level Match	RISB1757	Spodoptera frugiperda Order: Lepidoptera	degradation of lambda-cyhalothrin, deltamethrin, chlorpyrifos ethyl, lufenuron and spinosyn	0.03%	6.9
Candidatus Karelsulcia muelleri Species-level Match	RISB1591	Philaenus spumarius Order: Hemiptera	None	1.73%	6.7
Carnobacterium maltaromaticum Species-level Match	RISB1692	Plutella xylostella Order: Lepidoptera	participate in the synthesis of host lacking amino acids histidine and threonine	0.03%	6.6
Frischella perrara Species-level Match	RISB2028	Diceroprocta semicincta Order: Hemiptera	causes the formation of a scab-like structure on the gut epithelium of its host	0.01%	6.6
Candidatus Westeberhardia cardiocondylae Species-level Match	RISB1794	Cardiocondyla obscurior Order: Hymenoptera	Contributes to cuticle formation and is responsible for host invasive success	0.01%	6.6
Candidatus Walczuchella monophlebidarum Species-level Match	RISB2074	Llaveia axin axin Order: Hemiptera	may provide metabolic precursors to the flavobacterial endosymbiont	0.12%	6.5
Wigglesworthia glossinidia Species-level Match	RISB2577	Glossina brevipalpis Order: Diptera	provide its tsetse host with metabolites such as vitamins	0.07%	6.2
Leclercia adecarboxylata Species-level Match	RISB1758	Spodoptera frugiperda Order: Lepidoptera	may influence the metabolization of pesticides in insects	0.03%	6.2
Candidatus Westeberhardia cardiocondylae Species-level Match	RISB1795	Cardiocondyla obscurior Order: Hymenoptera	a contribution of Westeberhardia to cuticle formation	0.01%	6.1
Proteus vulgaris Species-level Match	RISB2460	Bombyx mori Order: Lepidoptera	degradation of cellulose, xylan, pectin and starch	0.05%	6.1
Lactiplantibacillus plantarum Species-level Match	RISB0674	Drosophila melanogaster Order: Diptera	could effectively inhibit fungal spore germinations	0.03%	6.1
Lysinibacillus fusiformis Species-level Match	RISB1417	Psammotermes hypostoma Order: Blattodea	isolates showed significant cellulolytic activity	0.05%	6.0
Providencia rettgeri Species-level Match	RISB1001	Anastrepha obliqua Order: Diptera	improve the sexual competitiveness of males	0.11%	6.0
Carnobacterium maltaromaticum Species-level Match	RISB1691	Plutella xylostella Order: Lepidoptera	activity of cellulose and hemicellulose	0.03%	5.8
Agrobacterium tumefaciens Species-level Match	RISB0650	Melanaphis bambusae Order: Hemiptera	None	0.75%	5.8
Providencia rettgeri Species-level Match	RISB1169	Bactrocera dorsalis Order: Diptera	Promote the growth of larvae	0.11%	5.7
Chryseobacterium sp. G0201 Species-level Match	RISB2092	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.07%	5.6
Chryseobacterium sp. T16E-39 Species-level Match	RISB2092	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.05%	5.6
Chryseobacterium sp. StRB126 Species-level Match	RISB2092	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.04%	5.6
Providencia alcalifaciens Species-level Match	RISB1168	Bactrocera dorsalis Order: Diptera	Promote the growth of larvae	0.01%	5.6
Comamonas testosteroni Species-level Match	RISB1875	Aedes aegypti Order: Diptera	gut microbiome	0.03%	5.3
Arsenophonus nasoniae Species-level Match	RISB0428	Nasonia vitripennis Order: Hymenoptera	male killing	0.01%	5.3
Candidatus Erwinia haradaeae Species-level Match	RISB1632	Lachninae Order: Hemiptera	None	0.09%	5.1
Flavobacterium johnsoniae Species-level Match	RISB0659	Melanaphis bambusae Order: Hemiptera	None	0.06%	5.1
Candidatus Annandia pinicola Species-level Match	RISB1661	Adelgidae Order: Hemiptera	None	0.04%	5.0
Lactiplantibacillus plantarum Species-level Match	RISB0608	Drosophila melanogaster Order: Diptera	None	0.03%	5.0
Candidatus Annandia adelgestsuga Species-level Match	RISB2207	Adelges tsugae Order: Hemiptera	None	0.03%	5.0
Variovorax sp. HW608 Species-level Match	RISB1712	Phlebotomus papatasi Order: Diptera	None	0.03%	5.0
Candidatus Carsonella ruddii Species-level Match	RISB0748	Diaphorina citri Order: Hemiptera	None	0.02%	5.0
Gilliamella apicola Species-level Match	RISB1945	Apis cerana Order: Hymenoptera	None	0.01%	5.0
Snodgrassella alvi Species-level Match	RISB1947	Apis cerana Order: Hymenoptera	None	0.01%	5.0
Arsenophonus nasoniae Species-level Match	RISB0366	Pachycrepoideus vindemmiae Order: Hymenoptera	None	0.01%	5.0
Candidatus Legionella polyplacis Species-level Match	RISB1687	Polyplax serrata Order: Phthiraptera	None	0.01%	5.0
Candidatus Cardinium	RISB0223	Bemisia tabaci Order: Hemiptera	Cardinium could inhibit the defense response of the host plant and decrease the detoxification metabolism ability of the host whitefly, decrease the expression of detoxification metabolism genes, especially the uridine 5'-diphospho-glucuronyltransferase and P450 genes,	0.01%	5.0
Candidatus Cardinium	RISB1439	Lutzomyia evansi Order: Diptera	‘Candidatus Cardinium’ is a recently described bacterium from the Bacteroidetes group involved in diverse reproduction alterations of its arthropod hosts (including cytoplasmic incompatibility, parthenogenesis, and feminization) similar to Wolbachia	0.01%	5.0
Treponema	RISB2377	termite Order: Blattodea	when grown together, two termite-gut Treponema species influence each other's gene expression in a far more comprehensive and nuanced manner than might have been predicted based on the results of previous studies on the respective pure cultures	0.05%	4.9
Apibacter	RISB0603	Apis cerana Order: Hymenoptera	The acquisition of genes for the degradation of the toxic monosaccharides potentiates Apibacter with the ability to utilize the pollen hydrolysis products, at the same time enabling monosaccharide detoxification for the host	0.17%	4.7
Rickettsiella	RISB2479	Acyrthosiphon pisum Order: Hemiptera	changes the insects’ body color from red to green in natural populations, the infection increased amounts of blue-green polycyclic quinones, whereas it had less of an effect on yellow-red carotenoid pigments	0.03%	4.2
Xanthomonas	RISB0498	Xylocopa appendiculata Order: Hymenoptera	Xanthomonas strain from Japanese carpenter bee is effective PU-degradable bacterium and is able to use polyacryl-based PU as a nutritional source, as well as other types of PS-PU and PE-PU	0.08%	3.8
Photorhabdus	RISB2532	Manduca sexta Order: Lepidoptera	produces a small-molecule antibiotic (E)-1,3-dihydroxy-2-(isopropyl)-5-(2-phenylethenyl)benzene (ST) that also acts as an inhibitor of phenoloxidase (PO) in the insect host Manduca sexta.	0.03%	3.8
Rickettsiella	RISB2262	Acyrthosiphon pisum Order: Hemiptera	against this entomopathogen Pandora neoaphidis, reduce mortality and also decrease fungal sporulation on dead aphids which may help protect nearby genetically identical insects	0.03%	3.6
Pectobacterium	RISB1889	Pseudococcus longispinus Order: Hemiptera	a nested symbiotic arrangement, where one bacterium lives inside another bacterium,occurred in building the mosaic metabolic pathways seen in mitochondria and plastids	0.04%	3.4
Methylobacter	RISB1440	Lutzomyia evansi Order: Diptera	Methylobacterium can be important in several physiological and metabolic processes in Lu. evansi, which suggests that interactions could occur with Leishmania parasite	0.01%	3.4
Candidatus Blochmanniella	RISB2542	Camponotus Order: Hymenoptera	Blochmannia provide essential amino acids to its host,Camponotus floridanus, and that it may also play a role in nitrogen recycling via its functional urease	0.14%	3.3
Candidatus Blochmanniella	RISB1827	Camponotus floridanus Order: Hymenoptera	a modulation of immune gene expression which may facilitate tolerance towards the endosymbionts and thus may contribute to their transovarial transmission	0.14%	3.2
Rickettsiella	RISB1739	Acyrthosiphon pisum Order: Hemiptera	in an experiment with a single-injected isolate of Rickettsiella sp. wasps were also attracted to plants fed on by aphids without secondary symbionts	0.03%	3.0
Ignatzschineria	RISB0562	Chrysomya megacephala Order: Diptera	Ignatzschineria indica is a Gram-negative bacterium commonly associated with maggot infestation and myiasis, a probable marker for myiasis diagnosis	0.01%	3.0
Bacteroides	RISB0256	Leptocybe invasa Order: Hymenoptera	Differences in Male-Killing Rickettsia Bacteria between Lineages of the Invasive Gall-Causing Pest Leptocybe invasa	0.58%	2.9
Candidatus Blochmanniella	RISB2448	Camponotus floridanus Order: Hymenoptera	nutritional contribution of the bacteria to host metabolism by production of essential amino acids and urease-mediated nitrogen recycling	0.14%	2.9
Photorhabdus	RISB2573	Manduca sexta Order: Lepidoptera	the bacteria are symbiotic with entomopathogenic nematodes but become pathogenic on release from the nematode into the insect blood system	0.03%	2.8
Shewanella	RISB1924	Anopheles gambiae Order: Diptera	may be related with mediating adaptation to different ecological niches or in shaping specific adult behaviors including mating	0.17%	2.7
Bacteroides	RISB0090	Hyphantria cunea Order: Lepidoptera	enhance the compatibility of invasive pests to new hosts and enable more rapid adaptation to new habitats.	0.58%	2.7
Yersinia	RISB0492	Cimex hemipterus Order: Hemiptera	the disruption of the abundant Yersinia possibly could be related to the enhanced susceptibility towards the insecticides	0.03%	2.5
Streptococcus	RISB2625	Galleria mellonella Order: Lepidoptera	suppress bacteria ingested with food by producing bacteriocin and by releasing a lysozyme like enzyme	0.35%	2.4
Liberibacter	RISB2310	Bactericerca cockerelli Order: Hemiptera	manipulate plant signaling and defensive responses, suppress accumulation of defense transcripts like JA and SA	0.01%	2.3
Blautia	RISB0091	Hyphantria cunea Order: Lepidoptera	enhance the compatibility of invasive pests to new hosts and enable more rapid adaptation to new habitats.	0.12%	2.2
Candidatus Cardinium	RISB2290	Sogatella furcifera Order: Hemiptera	dual infection with Cardinium and Wolbachia induced strong cytoplasmic incompatibility (CI) in a single host	0.01%	2.2
Coprococcus	RISB0092	Hyphantria cunea Order: Lepidoptera	enhance the compatibility of invasive pests to new hosts and enable more rapid adaptation to new habitats.	0.01%	2.1
Delftia	RISB0083	Osmia cornifrons Order: Hymenoptera	be known to exhibit antibiotic activity, suggesting their potential protective role against pathogens	0.06%	2.1
Xanthomonas	RISB0217	Xylocopa appendiculata Order: Hymenoptera	strains biodegraded polyethylene terephthalate PET powder, broke it into its degradation products	0.08%	2.0
Streptococcus	RISB2624	Reticulitermes flavipes Order: Blattodea	can be broken down into substances such as carbon dioxide, ammonia and acetic acid	0.35%	2.0
Xenorhabdus	RISB1372	Spodoptera frugiperda Order: Lepidoptera	the products of the symbiont gene cluster inhibit Spodoptera frugiperda phenoloxidase activity	0.02%	1.9
Liberibacter	RISB2524	Bactericera cockerelli Order: Hemiptera	Reduced expression of plant defensive gene in tomato probably for psyllid success	0.01%	1.6
Streptococcus	RISB2604	Homona magnanima Order: Lepidoptera	influence the growth of Bacillus thuringiensis in the larvae	0.35%	1.6
Xenorhabdus	RISB2270	Acyrthosiphon pisum Order: Hemiptera	have the gene PIN1 encoding the protease inhibitor protein against aphids	0.02%	1.5
Glutamicibacter	RISB0606	Phthorimaea operculella Order: Lepidoptera	could degrade the major toxic α-solanine and α-chaconine in potatoes	0.02%	1.4
Raoultella	RISB1672	Spodoptera frugiperda Order: Lepidoptera	downregulated POX but upregulated trypsin PI in this plant species	0.02%	1.3
Dysgonomonas	RISB1235	Hermetia illucens Order: Diptera	provides the tools for degrading of a broad range of substrates	0.04%	1.3
Photorhabdus	RISB0532	Drosophila melanogaster Order: Diptera	produces toxin complex (Tc) toxins as major virulence factors	0.03%	1.3
Delftia	RISB1754	Spodoptera frugiperda Order: Lepidoptera	may influence the metabolization of pesticides in insects	0.06%	1.2
Paraclostridium	RISB0028	Sesamia inferens Order: Lepidoptera	degrade Chlorpyrifos and Chlorantraniliprole in vitro	0.13%	1.2
Pectobacterium	RISB0798	Pseudoregma bambucicola Order: Hemiptera	may help P. bambucicola feed on the stalks of bamboo	0.04%	1.1
Dickeya	RISB1086	Rhodnius prolixus Order: Hemiptera	supply enzymatic biosynthesis of B-complex vitamins	0.05%	1.1
Liberibacter	RISB2333	Cacopsylla pyri Order: Hemiptera	behaves as an endophyte rather than a pathogen	0.01%	0.9
Aeromonas	RISB2456	Bombyx mori Order: Lepidoptera	able to utilize the CMcellulose and xylan	0.09%	0.9
Methylobacter	RISB2053	Atractomorpha sinensis Order: Orthoptera	associated with cellulolytic enzymes	0.01%	0.7
Aeromonas	RISB2086	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.09%	0.7
Priestia	RISB0839	Helicoverpa armigera Order: Lepidoptera	producing amylase	0.27%	0.6
Peribacillus	RISB1877	Aedes aegypti Order: Diptera	gut microbiome	0.13%	0.4
Methylobacter	RISB2340	Saturniidae Order: Lepidoptera	Nitrogen fixation	0.01%	0.4
Myroides	RISB0626	Musca altica Order: Diptera	None	0.34%	0.3
Sphingobium	RISB1880	Aedes aegypti Order: Diptera	gut microbiome	0.02%	0.3
Halomonas	RISB1374	Bemisia tabaci Order: Hemiptera	None	0.18%	0.2
Helicobacter	RISB0662	Melanaphis bambusae Order: Hemiptera	None	0.18%	0.2
Apibacter	RISB0604	Apis cerana Order: Hymenoptera	None	0.17%	0.2
Candidatus Phytoplasma	RISB1620	Cacopsylla pyricola Order: Hemiptera	None	0.10%	0.1
Neisseria	RISB0512	Plutella xylostella Order: Lepidoptera	None	0.06%	0.1
Metabacillus	RISB0902	Myzus persicae Order: Hemiptera	None	0.06%	0.1
Candidatus Arthromitus	RISB2613	Multiple species Order: None	None	0.06%	0.1
Treponema	RISB0169	Reticulitermes flaviceps Order: Blattodea	None	0.05%	0.1
Vagococcus	RISB0042	Aldrichina grahami Order: Diptera	None	0.05%	0.1
Pectobacterium	RISB1772	Muscidae Order: Diptera	None	0.04%	0.0
Sediminibacterium	RISB0244	Spodoptera frugiperda Order: Lepidoptera	None	0.04%	0.0
Yersinia	RISB0407	Anaphes nitens Order: Hymenoptera	None	0.03%	0.0
Cupriavidus	RISB0694	Alydus tomentosus Order: Hemiptera	None	0.03%	0.0
Glutamicibacter	RISB0438	Helicoverpa armigera Order: Lepidoptera	None	0.02%	0.0
Candidatus Profftia	RISB1664	Adelgidae Order: Hemiptera	None	0.02%	0.0
Ralstonia	RISB0243	Spodoptera frugiperda Order: Lepidoptera	None	0.02%	0.0
Candidatus Vallotia	RISB1665	Adelgidae Order: Hemiptera	None	0.02%	0.0
Geobacillus	RISB1251	Potamobates horvathi Order: Hemiptera	None	0.02%	0.0