SRR10017625 - Stolas sp.

Basic Information

Run: SRR10017625

Assay Type: WGS

Bioproject: PRJNA561424

Biosample: SAMN12618090

Bytes: 2792342598

Center Name: EMORY UNIVERSITY

Sequencing Information

Instrument: Illumina HiSeq 2500

Library Layout: PAIRED

Library Selection: RANDOM

Platform: ILLUMINA

Geographic Information

Country: Panama

Continent: North America

Location Name: Panama: Panama City

Latitude/Longitude: 8.9824 N 79.5199 W

Sample Information

Host: Stolas sp.

Isolation: -

Biosample Model: Metagenome or environmental

Collection Date: 2017-05-01

Taxonomic Classification

Potential Symbionts

About Potential Symbionts

This table shows potential symbiont identified in the metagenome sample. Matches are scored based on:

Relative abundance in the sample
Species-level matches with known symbionts
Host insect order matches with reference records
Completeness and richness of functional records

Note: only the three highest scoring comparison records were retained for each symbiont species or genus in the sample

Based on our current records database, this section aims to identify potential functional symbionts in this metagenome sample, with scoring based on:

Relative abundance in sample
Species-level matches with known symbionts
Host insect order matches
Functional record completeness

Note: Showing top 3 highest scoring records for each species/genus

Symbiont Name	Record	Host Species	Function	Abundance	Score Score Composition: Symbiont Abundance Species match bonus Host match bonus Function richness Higher scores indicate stronger symbiotic relationship potential
Enterobacter mori Species-level Match	RISB1163	Bactrocera dorsalis Order: Diptera	Promote the growth of larvae	27.75%	33.3
Staphylococcus gallinarum Species-level Match Host Order Match	RISB0945	Callosobruchus maculatus Order: Coleoptera	The strain encodes complete biosynthetic pathways for the production of B vitamins and amino acids, including tyrosine; A carbohydrate-active enzyme search revealed that the genome codes for a number of digestive enzymes, reflecting the nutritional ecology of C. maculatus	0.03%	20.0
Pantoea sp. JZ2 Species-level Match Host Order Match	RISB0736	Psylliodes chrysocephala Order: Coleoptera	Laboratory-reared and field-collected P. chrysocephala all contained three core genera Pantoea, Acinetobacter and Pseudomonas, and reintroduction of Pantoea sp. Pc8 in antibiotic-fed beetles restored isothiocyanate degradation ability in vivo (by 16S rRNA gene sequencing and LC-MS)	0.02%	20.0
Pantoea sp. BRR-3P Species-level Match Host Order Match	RISB0736	Psylliodes chrysocephala Order: Coleoptera	Laboratory-reared and field-collected P. chrysocephala all contained three core genera Pantoea, Acinetobacter and Pseudomonas, and reintroduction of Pantoea sp. Pc8 in antibiotic-fed beetles restored isothiocyanate degradation ability in vivo (by 16S rRNA gene sequencing and LC-MS)	0.01%	20.0
Pseudomonas sp. CIP-10 Species-level Match Host Order Match	RISB1622	Dendroctonus valens Order: Coleoptera	volatiles from predominant bacteria regulate the consumption sequence of carbon sources d-pinitol and d-glucose in the fungal symbiont Leptographium procerum, and appear to alleviate the antagonistic effect from the fungus against RTB larvae	0.16%	20.0
Pseudomonas sp. RtIB026 Species-level Match Host Order Match	RISB1622	Dendroctonus valens Order: Coleoptera	volatiles from predominant bacteria regulate the consumption sequence of carbon sources d-pinitol and d-glucose in the fungal symbiont Leptographium procerum, and appear to alleviate the antagonistic effect from the fungus against RTB larvae	0.03%	19.9
Serratia liquefaciens Species-level Match Host Order Match	RISB1624	Dendroctonus valens Order: Coleoptera	volatiles from predominant bacteria regulate the consumption sequence of carbon sources d-pinitol and d-glucose in the fungal symbiont Leptographium procerum, and appear to alleviate the antagonistic effect from the fungus against RTB larvae	0.01%	19.8
Acinetobacter sp. Tol 5 Species-level Match Host Order Match	RISB0730	Curculio chinensis Order: Coleoptera	Acinetobacter sp. in C. chinensis enriched after treating with saponin, and when incubating bacteria with saponin for 72 h, saponin content significantly decreased from 4.054 to 1.867 mg/mL (by 16S rRNA metagenome sequencing and HPLC)	0.07%	19.8
Acinetobacter sp. Marseille-Q1620 Species-level Match Host Order Match	RISB0730	Curculio chinensis Order: Coleoptera	Acinetobacter sp. in C. chinensis enriched after treating with saponin, and when incubating bacteria with saponin for 72 h, saponin content significantly decreased from 4.054 to 1.867 mg/mL (by 16S rRNA metagenome sequencing and HPLC)	0.02%	19.7
Acinetobacter sp. Z1 Species-level Match Host Order Match	RISB0730	Curculio chinensis Order: Coleoptera	Acinetobacter sp. in C. chinensis enriched after treating with saponin, and when incubating bacteria with saponin for 72 h, saponin content significantly decreased from 4.054 to 1.867 mg/mL (by 16S rRNA metagenome sequencing and HPLC)	0.01%	19.7
Escherichia coli Species-level Match Host Order Match	RISB0128	Tribolium castaneum Order: Coleoptera	may produce 4,8-dimethyldecanal (DMD) production that is strongly associated with attraction to females and host pheromone communication	1.78%	19.5
Pantoea ananatis Species-level Match	RISB1671	Spodoptera frugiperda Order: Lepidoptera	modulate plant defense, downregulated the activity of the plant defensive proteins polyphenol oxidase and trypsin proteinase inhibitors (trypsin PI) but upregulated peroxidase (POX) activity in tomatoresponses	10.26%	19.4
Citrobacter freundii Species-level Match Host Order Match	RISB0517	Leptinotarsa decemlineata Order: Coleoptera	affect the cellular and humoral immunity of the insect, increasing its susceptibility to Bacillus thuringiensis var. tenebrionis (morrisoni) (Bt)	0.88%	18.8
Lactococcus lactis Species-level Match Host Order Match	RISB0967	Oulema melanopus Order: Coleoptera	contribute to the decomposition of complex carbohydrates, fatty acids, or polysaccharides in the insect gut. It might also contribute to the improvement of nutrient availability.	0.09%	18.7
Citrobacter freundii Species-level Match Host Order Match	RISB0127	Tribolium castaneum Order: Coleoptera	may produce 4,8-dimethyldecanal (DMD) production that is strongly associated with attraction to females and host pheromone communication	0.88%	18.6
Pseudomonas sp. CIP-10 Species-level Match Host Order Match	RISB2224	Leptinotarsa decemlineata Order: Coleoptera	Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)	0.16%	18.5
Enterobacter sp. JBIWA003 Species-level Match Host Order Match	RISB2221	Leptinotarsa decemlineata Order: Coleoptera	Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)	0.04%	18.4
Enterobacter sp. JJBC Species-level Match Host Order Match	RISB2221	Leptinotarsa decemlineata Order: Coleoptera	Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)	0.03%	18.4
Sphingobacterium sp. SYP-B4668 Species-level Match Host Order Match	RISB2227	Leptinotarsa decemlineata Order: Coleoptera	Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)	0.02%	18.4
Sphingobacterium sp. LZ7M1 Species-level Match Host Order Match	RISB2227	Leptinotarsa decemlineata Order: Coleoptera	Colorado potato beetle (Leptinotarsa decemlineata) larvae exploit bacteria in their oral secretions to suppress antiherbivore defenses in tomato (Solanum lycopersicum)	0.01%	18.4
Klebsiella oxytoca Species-level Match Host Order Match	RISB1506	Cleonus trivittatus Order: Coleoptera	Antibiotic-treated larvae suffered growth retardation on a diet containing plant extract or swainsonine. Gut bacteria showed toxin-degradation activities in vitro	0.01%	18.3
Serratia marcescens Species-level Match Host Order Match	RISB1295	Nicrophorus vespilloides Order: Coleoptera	producing antibacterial compound Serrawettin W2, which has antibacterial and nematode-inhibiting effects	0.85%	17.9
Morganella morganii Species-level Match Host Order Match	RISB1867	Costelytra zealandica Order: Coleoptera	Female beetles were previously shown to use phenol as their sex pheromone produced by symbiotic bacteria in the accessory or colleterial gland	0.02%	17.9
Proteus vulgaris Species-level Match Host Order Match	RISB0001	Leptinotarsa decemlineata Order: Coleoptera	produces toxic hydrogen cyanide (HCN) and a mandelonitrile-producing cyanoglucoside, amygdalin, which protect the insect from predation	0.04%	17.7
Bacillus sp. JAS24-2 Species-level Match Host Order Match	RISB1645	Osphranteria coerulescens Order: Coleoptera	The isolate has cellulolytic activity and can hydrolyze CMC, avicel, cellulose and sawdust with broad temperature and pH stability	0.07%	17.7
Serratia marcescens Species-level Match Host Order Match	RISB0365	Pagiophloeus tsushimanus Order: Coleoptera	terpenoid-degrading: the highest degradation rates of D-camphor, linalool, and eucalyptol	0.85%	17.6
Enterococcus faecalis Species-level Match Host Order Match	RISB0497	Cryptolestes ferrugineus Order: Coleoptera	bacteria can degrade malathion, pirimiphos-methyl, and deltamethrin and utilize these insecticides as the carbon source in vitro.	0.05%	17.6
Bacillus subtilis Species-level Match Host Order Match	RISB0494	Sitophilus oryzae Order: Coleoptera	bacteria can degrade malathion, pirimiphos-methyl, and deltamethrin and utilize these insecticides as the carbon source in vitro.	0.04%	17.6
Bacillus sp. 1NLA3E Species-level Match Host Order Match	RISB1645	Osphranteria coerulescens Order: Coleoptera	The isolate has cellulolytic activity and can hydrolyze CMC, avicel, cellulose and sawdust with broad temperature and pH stability	0.01%	17.6
Staphylococcus gallinarum Species-level Match Host Order Match	RISB0946	Callosobruchus maculatus Order: Coleoptera	The strain encodes complete biosynthetic pathways for the production of B vitamins and amino acids, including tyrosine	0.03%	17.4
Enterococcus faecalis Species-level Match Host Order Match	RISB2042	Harpalus pensylvanicus Order: Coleoptera	E. faecalis facilitate seed consumption by H. pensylvanicus, possibly by contributing digestive enzymes to their host	0.05%	17.4
Buchnera aphidicola Species-level Match	RISB0236	Acyrthosiphon pisum Order: Hemiptera	Buchnera the nutritional endosymbiont of A. pisum is located inside of bacteriocytes and requires aspartate from the aphid host, because it cannot make it de novo. Further Buchnera needs aspartate for the biosynthesis of the essential amino acids lysine and threonine, which the aphid and Buchnera require for survival	7.39%	17.4
Buchnera aphidicola Species-level Match	RISB2485	Macrosiphum euphorbiae Order: Hemiptera	symbiont expression patterns differ between aphid clones with differing levels of virulence, and are influenced by the aphids' host plant. Potentially, symbionts may contribute to differential adaptation of aphids to host plant resistance	7.39%	17.2
Lactococcus lactis Species-level Match Host Order Match	RISB1430	Rhynchophorus ferrugineus Order: Coleoptera	promote the development and body mass gain of RPW larvae by improving their nutrition metabolism	0.09%	17.0
Morganella morganii Species-level Match Host Order Match	RISB1548	Costelytra zealandica Order: Coleoptera	symbionts residing in the colleterial glands produce phenol 1 as the female sex pheromone	0.02%	16.8
Morganella morganii Species-level Match Host Order Match	RISB1868	Costelytra zealandica Order: Coleoptera	produces phenol as the sex pheromone of the host from tyrosine in the colleterial gland	0.02%	16.8
Streptomyces sp. T12 Species-level Match Host Order Match	RISB0777	Copris tripartitus Order: Coleoptera	contribute brood ball hygiene by inhibiting fungal parasites in the environment	0.07%	16.7
Streptomyces sp. MST-110588 Species-level Match Host Order Match	RISB0777	Copris tripartitus Order: Coleoptera	contribute brood ball hygiene by inhibiting fungal parasites in the environment	0.03%	16.6
Streptomyces sp. SCSIO 75703 Species-level Match Host Order Match	RISB0777	Copris tripartitus Order: Coleoptera	contribute brood ball hygiene by inhibiting fungal parasites in the environment	0.01%	16.6
Enterococcus faecalis Species-level Match Host Order Match	RISB0374	Tribolium castaneum Order: Coleoptera	modulates host phosphine resistance by interfering with the redox system	0.05%	16.5
Paenibacillus sp. FSL K6-3182 Species-level Match Host Order Match	RISB0813	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-9 oxidation pathway	0.05%	16.5
Paenibacillus sp. DCT19 Species-level Match Host Order Match	RISB0813	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-9 oxidation pathway	0.03%	16.4
Paenibacillus sp. HWE-109 Species-level Match Host Order Match	RISB0813	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-9 oxidation pathway	0.01%	16.4
Buchnera aphidicola Species-level Match	RISB0685	Acyrthosiphon pisum Order: Hemiptera	It supplies the host with vitamins and essential amino acids, such as arginine and methionine that aphids cannot synthesize or derive insufficiently from their diet, the phloem sap of plants	7.39%	16.2
Stenotrophomonas maltophilia Species-level Match Host Order Match	RISB0139	Tenebrio molitor Order: Coleoptera	correlated with polyvinyl chloride PVC degradation	0.07%	16.1
Citrobacter koseri Species-level Match Host Order Match	RISB1060	Oryctes rhinoceros Order: Coleoptera	associated with insect digestive tracts	0.02%	15.8
Wolbachia pipientis Species-level Match Host Order Match	RISB2621	Tribolium confusum Order: Coleoptera	induces cytoplasmic incompatibility	0.02%	15.7
Klebsiella pneumoniae Species-level Match Host Order Match	RISB1153	Tenebrio molitor Order: Coleoptera	degrading plastics	0.35%	15.7
Exiguobacterium sp. MH3 Species-level Match Host Order Match	RISB1152	Tenebrio molitor Order: Coleoptera	degrading plastics	0.04%	15.4
Lactococcus lactis Species-level Match Host Order Match	RISB1065	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.09%	15.3
Staphylococcus hominis Species-level Match Host Order Match	RISB1071	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.04%	15.3
Lysinibacillus fusiformis Species-level Match Host Order Match	RISB1066	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.01%	15.2
Burkholderia Host Order Match	RISB1172	Lagria villosa Order: Coleoptera	process a cryptic gene cluster that codes for the biosynthesis of a novel antifungal polyketide with a glutarimide pharmacophore, which led to the discovery of the gladiofungins as previously-overlooked components of the antimicrobial armory of the beetle symbiont	0.01%	15.0
Burkholderia Host Order Match	RISB1729	Lagria hirta Order: Coleoptera	the symbionts inhibit the growth of antagonistic fungi on the eggs of the insect host, indicating that the Lagria-associated Burkholderia have evolved from plant pathogenic ancestors into insect defensive mutualists	0.01%	14.3
Burkholderia Host Order Match	RISB1836	Dendroctonus valens Order: Coleoptera	It can trongly degrade naringenin, and pinitol, the main soluble carbohydrate of P. tabuliformis, is retained in L. procerum-infected phloem and facilitate naringenin biodegradation by the microbiotas.	0.01%	14.0
Sodalis Host Order Match	RISB2035	Sitophilus oryzae Order: Coleoptera	endosymbiont dynamics parallels numerous transcriptional changes in weevil developing adults and affects several biological processes, including metabolism and development	0.01%	13.4
Sodalis Host Order Match	RISB2607	Sitophilus oryzae Order: Coleoptera	induces the specific differentiation of the bacteriocytes, increases mitochondrial oxidative phosphorylation through the supply of pantothenic acid and riboflavin	0.01%	13.3
Sodalis Host Order Match	RISB1718	Sitophilus zeamais Order: Coleoptera	we investigated the role of a quorum sensing（QS ） system in S. praecaptivus and found that it negatively regulates a potent insect-killing phenotype	0.01%	13.0
Candidatus Nardonella Host Order Match	RISB2449	Euscepes postfasciatus Order: Coleoptera	endosymbiont is involved in normal growth and development of the host weevil	0.90%	12.4
Candidatus Nardonella Host Order Match	RISB1931	Lissorhoptrus oryzophilus Order: Coleoptera	might be not playing critical roles in the reproduction of L. oryzophilus	0.90%	12.4
Bacteroides Host Order Match	RISB1183	Oryzaephilus surinamensis Order: Coleoptera	supplement precursors for the cuticle synthesis and thereby enhance desiccation resistance of its host	0.30%	12.3
Vibrio Host Order Match	RISB1810	Monochamus galloprovincialis Order: Coleoptera	Have the ability for degradation of cellulose, proteins and starch	0.48%	11.8
Candidatus Nardonella Host Order Match	RISB1668	Multiple species Order: Coleoptera	Possibly tyrosine precursor provisioning	0.90%	11.7
Rhizobium Host Order Match	RISB0135	Coccinella septempunctata Order: Coleoptera	be commonly found in plant roots and they all have nitrogen fixation abilities	0.11%	11.7
Nostoc Host Order Match	RISB0812	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-18 oxidation pathway	0.21%	11.6
Leuconostoc Host Order Match	RISB0812	Hypothenemus hampei Order: Coleoptera	might contribute to caffeine breakdown using the C-18 oxidation pathway	0.03%	11.5
Candidatus Mesenet Host Order Match	RISB1785	Brontispa longissima Order: Coleoptera	induced complete Cytoplasmic incompatibility (CI) (100% mortality)	0.01%	11.3
Escherichia coli Species-level Match	RISB1339	Manduca sexta Order: Lepidoptera	modulate immunity-related gene expression in the infected F0 larvae, and also in their offspring, triggered immune responses in the infected host associated with shifts in both DNA methylation and histone acetylation	1.78%	11.1
Cronobacter Host Order Match	RISB0247	Tenebrio molitor Order: Coleoptera	may be indirectly involved in the digestion of PE	0.02%	11.0
Mycobacterium Host Order Match	RISB1156	Nicrophorus concolor Order: Coleoptera	produces Antimicrobial compounds	0.02%	10.7
Turicibacter Host Order Match	RISB0451	Odontotaenius disjunctus Order: Coleoptera	degrading ellulose and xylan	0.02%	10.6
Aeromonas Host Order Match	RISB1145	Tenebrio molitor Order: Coleoptera	degrading plastics	0.03%	10.4
Klebsiella pneumoniae Species-level Match	RISB2185	Scirpophaga incertulas Order: Lepidoptera	The ability of these arthropods to feed on wood, foliage and detritus is likely to involve catalysis by different types of cellulases/hemicellulases that are secreted by gut microbiota to digest the structural and recalcitrant lignocellulosic residues in their foods.	0.35%	10.4
Comamonas Host Order Match	RISB1061	Oryctes rhinoceros Order: Coleoptera	gut microbe	0.03%	10.3
Wolbachia pipientis Species-level Match	RISB0766	Aedes fluviatilis Order: Diptera	The presence of Wolbachia pipientis improves energy performance in A. fluviatilis cells; it affects the regulation of key energy sources such as lipids, proteins, and carbohydrates, making the distribution of actin more peripheral and with extensions that come into contact with neighboring cells.	0.02%	10.0
Listeria monocytogenes Species-level Match	RISB2308	Drosophila melanogaster Order: Diptera	L. monocytogenes infection disrupts host energy metabolism by depleting energy stores (triglycerides and glycogen) and reducing metabolic pathway activity (beta-oxidation and glycolysis). The infection affects antioxidant defense by reducing uric acid levels and alters amino acid metabolism. These metabolic changes are accompanied by melanization, potentially linked to decreased tyrosine levels.	0.02%	10.0
Micromonospora Host Order Match	RISB2034	Harpalus sinicus Order: Coleoptera	None	0.02%	10.0
Francisella tularensis Species-level Match	RISB1907	Bombyx mori Order: Lepidoptera	After infection with F. tularensis, the induction of melanization and nodulation, which are immune responses to bacterial infection, were inhibited in silkworms. Pre-inoculation of silkworms with F. tularensis enhanced the expression of antimicrobial peptides and resistance to infection by pathogenic bacteria.	0.01%	10.0
Gilliamella apicola Species-level Match	RISB0102	Apis mellifera Order: Hymenoptera	Gilliamella apicola carries the gene for the desaturase FADS2, which is able to metabolize polyunsaturated fatty acids from pollen and synthesize endocannabinoid, a lipogenic neuroactive substance, thereby modulating reward learning and memory in honeybees.	0.01%	10.0
Candidatus Liberibacter asiaticus Species-level Match	RISB1077	Diaphorina citri Order: Hemiptera	CLas exposure altered the abundance of proteins involved in immunity and cellular and oxidative stress in a sex-dependent manner. Also, Clas impacted cuticular proteins and enzymes involved in chitin degradation, as well as energy metabolism and abundance of the endosymbiont 'Candidatus Profftella armatura' in both sexes similarly	0.01%	10.0
Dysgonomonas Host Order Match	RISB1481	Brachinus elongatulus Order: Coleoptera	None	0.01%	10.0
Treponema primitia Species-level Match	RISB2377	termite Order: Blattodea	when grown together, two termite-gut Treponema species influence each other's gene expression in a far more comprehensive and nuanced manner than might have been predicted based on the results of previous studies on the respective pure cultures	0.01%	9.9
Candidatus Nasuia deltocephalinicola Species-level Match	RISB2283	Nephotettix cincticeps Order: Hemiptera	Oral administration of tetracycline to nymphal N. cincticeps resulted in retarded growth, high mortality rates, and failure in adult emergence, suggesting important biological roles of the symbionts for the host insect	0.01%	9.4
Clostridium sp. MB40-C1 Species-level Match	RISB2301	Pyrrhocoris apterus Order: Hemiptera	could play an important role for the insect by degrading complex dietary components, providing nutrient supplementation, or detoxifying noxious chemicals (e.g. cyclopropenoic fatty acids or gossypol) in the diet	0.06%	9.3
Clostridium sp. DL-VIII Species-level Match	RISB2301	Pyrrhocoris apterus Order: Hemiptera	could play an important role for the insect by degrading complex dietary components, providing nutrient supplementation, or detoxifying noxious chemicals (e.g. cyclopropenoic fatty acids or gossypol) in the diet	0.05%	9.3
Clostridium sp. BJN0001 Species-level Match	RISB2301	Pyrrhocoris apterus Order: Hemiptera	could play an important role for the insect by degrading complex dietary components, providing nutrient supplementation, or detoxifying noxious chemicals (e.g. cyclopropenoic fatty acids or gossypol) in the diet	0.03%	9.3
Candidatus Schneideria nysicola Species-level Match	RISB0872	Nysius sp. Order: Hemiptera	synthesize four B vitamins(Pan, pantothenate;Fol, folate; Rib, riboflavin; Pyr, pyridoxine) and five Essential Amino Acids(Ile, isoleucine; Val, valine; Lys, lysine; Thr, threonine; Phe, phenylalanine)	0.08%	9.1
Candidatus Carsonella ruddii Species-level Match	RISB0394	Cacopsylla pyricola Order: Hemiptera	Carsonella produces most essential amino acids (EAAs) for C. pyricola, Psyllophila complements the genes missing in Carsonella for the tryptophan pathway and synthesizes some vitamins and carotenoids	0.12%	9.1
Stenotrophomonas maltophilia Species-level Match	RISB1122	Bombyx mori Order: Lepidoptera	facilitate host resistance against organophosphate insecticides, provides essential amino acids that increase host fitness and allow the larvae to better tolerate the toxic effects of the insecticide.	0.07%	9.1
Mammaliicoccus sciuri Species-level Match	RISB0075	Bombyx mori Order: Lepidoptera	could produce a secreted chitinolytic lysozyme (termed Msp1) to damage fungal cell walls，completely inhibit the spore germination of fungal entomopathogens Metarhizium robertsii and Beauveria bassiana	0.06%	9.1
Weissella cibaria Species-level Match	RISB1982	Blattella germanica Order: Blattodea	gut microbiota contributes to production of VCAs that act as fecal aggregation agents and that cockroaches discriminate among the complex odors that emanate from a diverse microbial community	0.01%	8.8
Candidatus Portiera aleyrodidarum Species-level Match	RISB1193	Bemisia tabaci Order: Hemiptera	synthesizing essential amino acid (e.g. tryptophan, leucine and L-Isoleucine), Bemisia tabaci provides vital nutritional support for growth, development and reproduction	0.29%	8.7
Lactobacillus sp. ESL0785 Species-level Match	RISB0292	Lymantria dispar asiatica Order: Lepidoptera	Beauveria bassiana infection-based assays showed that the mortality of non-axenic L. dispar asiatica larvae was significantly higher than that of axenic larvae at 72 h.	0.05%	8.4
Lactobacillus sp. PV034 Species-level Match	RISB0292	Lymantria dispar asiatica Order: Lepidoptera	Beauveria bassiana infection-based assays showed that the mortality of non-axenic L. dispar asiatica larvae was significantly higher than that of axenic larvae at 72 h.	0.02%	8.4
Candidatus Mikella endobia Species-level Match	RISB1887	Paracoccus marginatus Order: Hemiptera	a nested symbiotic arrangement, where one bacterium lives inside another bacterium,occurred in building the mosaic metabolic pathways seen in mitochondria and plastids	0.02%	8.4
Candidatus Gullanella endobia Species-level Match	RISB1885	Ferrisia virgata Order: Hemiptera	a nested symbiotic arrangement, where one bacterium lives inside another bacterium,occurred in building the mosaic metabolic pathways seen in mitochondria and plastids	0.01%	8.4
Spiroplasma sp. BIUS-1 Species-level Match	RISB1353	Cephus cinctus Order: Hymenoptera	The bacterium also encoded biosynthetic pathways for essential vitamins B2, B3, and B9. We identified putative Spiroplasma virulence genes: cardiolipin and chitinase.	0.01%	8.3
Blattabacterium cuenoti Species-level Match	RISB0133	Panesthiinae Order: Blattodea	enables hosts to subsist on a nutrient-poor diet; endosymbiont genome erosions are associated with repeated host transitions to an underground life	0.38%	8.3
Wigglesworthia glossinidia Species-level Match	RISB0369	Glossina morsitans Order: Diptera	symbiont-derived factors, likely B vitamins, are critical for the proper function of both lipid biosynthesis and lipolysis to maintain tsetse fly fecundity	0.05%	8.2
Candidatus Profftella armatura (Diaphorina cf. continua) Species-level Match	RISB2147	Diaphorina citri Order: Hemiptera	a defensive symbiont presumably of an obligate nature, which encoded horizontally acquired genes for synthesizing a novel polyketide toxin, diaphorin	0.04%	8.0
Candidatus Moranella endobia Species-level Match	RISB2232	Planococcus citri Order: Hemiptera	be responsible for the biosynthesis of most cellular components and energy provision, and controls most informational processes for the consortium	0.03%	8.0
Spiroplasma poulsonii Species-level Match	RISB1346	Drosophila melanogaster Order: Diptera	S. poulsonii protects its host against parasitoid wasps and nematodes by the action of toxins from the family of Ribosome Inactivating Proteins	0.01%	7.9
Candidatus Profftella armatura (Diaphorina cf. continua) Species-level Match	RISB2005	Diaphorina citri Order: Hemiptera	produce proteins involved in polyketide biosynthesis,which were up-regulated in CLas(+) insects (associated with citrus greening disease)	0.04%	7.8
Stenotrophomonas maltophilia Species-level Match	RISB1227	Delia antiqua Order: Diptera	six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana through symbiotic bacterium-derived organic acids	0.07%	7.8
Weissella cibaria Species-level Match	RISB0641	Formica Order: Hymenoptera	exhibited abilities in catabolizing sugars (sucrose, trehalose, melezitose and raffinose) known to be constituents of hemipteran honeydew	0.01%	7.8
Sphingobacterium faecium Species-level Match	RISB1226	Delia antiqua Order: Diptera	six bacteria protect larvae from infection with the entomopathogen Beauveria bassiana through symbiotic bacterium-derived organic acids	0.02%	7.7
Exiguobacterium sp. MH3 Species-level Match	RISB0007	Phormia regina Order: Diptera	prompted oviposition by flies; The flies' oviposition decisions appear to be guided by bacteria-derived semiochemicals as the bacteria	0.04%	7.7
Escherichia coli Species-level Match	RISB2120	Galleria mellonella Order: Lepidoptera	mediate trans-generational immune priming	1.78%	7.6
Spiroplasma ixodetis Species-level Match	RISB0842	Dactylopius coccus Order: Hemiptera	use the T4SS to interact with the Dactylopius cells, which show a strong interaction and molecular signaling in the symbiosis	0.10%	7.6
Wolbachia pipientis Species-level Match	RISB1515	Drosophila melanogaster Order: Diptera	increases the recombination rate observed across two genomic intervals and increases the efficacy of natural selection in hosts	0.02%	7.6
Carnobacterium maltaromaticum Species-level Match	RISB1693	Plutella xylostella Order: Lepidoptera	play an important role in the breakdown of plant cell walls, detoxification of plant phenolics, and synthesis of amino acids.	0.02%	7.5
Candidatus Nasuia deltocephalinicola Species-level Match	RISB2282	Nephotettix cincticeps Order: Hemiptera	With the antibiotic, nymphal growth was remarkably retarded, and a number of nymphs either died or failed to attain adulthood	0.01%	7.5
Candidatus Portiera aleyrodidarum Species-level Match	RISB2289	Bemisia tabaci Order: Hemiptera	encoding the capability to synthetize, or participate in the synthesis of, several amino acids and carotenoids,	0.29%	7.5
Candidatus Profftella armatura (Diaphorina cf. continua) Species-level Match	RISB2146	Diaphorina citri Order: Hemiptera	encoded horizontally acquired genes for synthesizing a novel polyketide toxin, providing defense against natural enemies	0.04%	7.4
Candidatus Tachikawaea gelatinosa Species-level Match	RISB2112	Urostylis westwoodii Order: Hemiptera	the symbiont localizes to a specialized midgut region and supplies essential amino acids deficient in the host's diet	0.09%	7.4
Candidatus Nasuia deltocephalinicola Species-level Match	RISB0262	Maiestas dorsalis Order: Hemiptera	are responsible for synthesizing two essential amino acids (histidine and methionine) and riboflavin (vitamin B2)	0.01%	7.3
Candidatus Portiera aleyrodidarum Species-level Match	RISB1973	Bemisia tabaci Order: Hemiptera	a primary symbiont, which compensates for the deficient nutritional composition of its food sources	0.29%	7.3
Candidatus Rickettsiella viridis Species-level Match	RISB1949	Acyrthosiphon pisum Order: Hemiptera	young red aphid larvae infected whith symbiont become greener at adulthood,which can reduce predation risk	0.03%	7.2
Wigglesworthia glossinidia Species-level Match	RISB1786	Glossina morsitans Order: Diptera	Synthesis of a large number of B vitamins, to supplement the host nutritional deficiencies of the diet	0.05%	7.1
Candidatus Ishikawella capsulata Species-level Match	RISB2368	Megacopta punctatissima Order: Hemiptera	Microbe compensates for nutritional deficiency of host diet by supplying essential amino acids	0.09%	7.0
Leclercia adecarboxylata Species-level Match	RISB1757	Spodoptera frugiperda Order: Lepidoptera	degradation of lambda-cyhalothrin, deltamethrin, chlorpyrifos ethyl, lufenuron and spinosyn	0.05%	6.9
Blattabacterium sp. (Cryptocercus punctulatus) str. Cpu Species-level Match	RISB1534	Periplaneta fuliginosa Order: Blattodea	involved in uric acid degradation, nitrogen assimilation and nutrient provisioning	0.06%	6.7
Blattabacterium sp. (Mastotermes darwiniensis) Species-level Match	RISB1534	Periplaneta fuliginosa Order: Blattodea	involved in uric acid degradation, nitrogen assimilation and nutrient provisioning	0.01%	6.7
Carnobacterium maltaromaticum Species-level Match	RISB1692	Plutella xylostella Order: Lepidoptera	participate in the synthesis of host lacking amino acids histidine and threonine	0.02%	6.6
Candidatus Westeberhardia cardiocondylae Species-level Match	RISB1794	Cardiocondyla obscurior Order: Hymenoptera	Contributes to cuticle formation and is responsible for host invasive success	0.07%	6.6
Frischella perrara Species-level Match	RISB2028	Diceroprocta semicincta Order: Hemiptera	causes the formation of a scab-like structure on the gut epithelium of its host	0.01%	6.6
Candidatus Rickettsiella viridis Species-level Match	RISB0277	Myzus persicae Order: Hemiptera	parasitoids showing a preference for probing aphids infected with R. viridis	0.03%	6.6
Xenorhabdus bovienii Species-level Match	RISB2270	Acyrthosiphon pisum Order: Hemiptera	have the gene PIN1 encoding the protease inhibitor protein against aphids	0.02%	6.5
Wigglesworthia glossinidia Species-level Match	RISB2577	Glossina brevipalpis Order: Diptera	provide its tsetse host with metabolites such as vitamins	0.05%	6.2
Leclercia adecarboxylata Species-level Match	RISB1758	Spodoptera frugiperda Order: Lepidoptera	may influence the metabolization of pesticides in insects	0.05%	6.2
Candidatus Riesia pediculicola Species-level Match	RISB2452	Pediculus humanus humanus Order: Phthiraptera	supplement body lice nutritionally deficient blood diet	0.08%	6.2
Delftia lacustris Species-level Match	RISB1754	Spodoptera frugiperda Order: Lepidoptera	may influence the metabolization of pesticides in insects	0.04%	6.2
Candidatus Westeberhardia cardiocondylae Species-level Match	RISB1795	Cardiocondyla obscurior Order: Hymenoptera	a contribution of Westeberhardia to cuticle formation	0.07%	6.1
Proteus vulgaris Species-level Match	RISB2460	Bombyx mori Order: Lepidoptera	degradation of cellulose, xylan, pectin and starch	0.04%	6.0
Lactiplantibacillus plantarum Species-level Match	RISB0674	Drosophila melanogaster Order: Diptera	could effectively inhibit fungal spore germinations	0.02%	6.0
Lysinibacillus fusiformis Species-level Match	RISB1417	Psammotermes hypostoma Order: Blattodea	isolates showed significant cellulolytic activity	0.01%	6.0
Providencia rettgeri Species-level Match	RISB1001	Anastrepha obliqua Order: Diptera	improve the sexual competitiveness of males	0.09%	6.0
Candidatus Ishikawella capsulata Species-level Match	RISB2543	Megacopta punctatissima Order: Hemiptera	Enhance pest status of the insect host	0.09%	5.9
Carnobacterium maltaromaticum Species-level Match	RISB1691	Plutella xylostella Order: Lepidoptera	activity of cellulose and hemicellulose	0.02%	5.8
Providencia sp. R33 Species-level Match	RISB1574	Bactrocera tau Order: Diptera	could attract male and female B. tau	0.02%	5.7
Providencia sp. R33 Species-level Match	RISB0984	Nasonia vitripennis Order: Hymenoptera	may highly associated with diapause	0.02%	5.7
Chryseobacterium sp. T16E-39 Species-level Match	RISB2092	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.05%	5.6
Chryseobacterium sp. D764 Species-level Match	RISB2092	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.03%	5.6
Chryseobacterium sp. CY350 Species-level Match	RISB2092	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.02%	5.6
Salmonella enterica Species-level Match	RISB0413	Melanaphis sacchari Order: Hemiptera	None	0.38%	5.4
Bombilactobacillus bombi Species-level Match	RISB0617	Spodoptera frugiperda Order: Lepidoptera	degrade amygdalin	0.01%	5.4
Candidatus Annandia pinicola Species-level Match	RISB1661	Adelgidae Order: Hemiptera	None	0.28%	5.3
Arsenophonus nasoniae Species-level Match	RISB0428	Nasonia vitripennis Order: Hymenoptera	male killing	0.02%	5.3
Candidatus Annandia adelgestsuga Species-level Match	RISB2207	Adelges tsugae Order: Hemiptera	None	0.14%	5.1
Candidatus Erwinia haradaeae Species-level Match	RISB1632	Lachninae Order: Hemiptera	None	0.14%	5.1
Candidatus Carsonella ruddii Species-level Match	RISB0748	Diaphorina citri Order: Hemiptera	None	0.12%	5.1
Candidatus Legionella polyplacis Species-level Match	RISB1687	Polyplax serrata Order: Phthiraptera	None	0.07%	5.1
Candidatus Palibaumannia cicadellinicola Species-level Match	RISB1594	Graphocephala coccinea Order: Hemiptera	None	0.06%	5.1
Agrobacterium tumefaciens Species-level Match	RISB0650	Melanaphis bambusae Order: Hemiptera	None	0.06%	5.1
Flavobacterium johnsoniae Species-level Match	RISB0659	Melanaphis bambusae Order: Hemiptera	None	0.05%	5.1
Candidatus Karelsulcia muelleri Species-level Match	RISB1591	Philaenus spumarius Order: Hemiptera	None	0.05%	5.1
Delftia lacustris Species-level Match	RISB0657	Melanaphis bambusae Order: Hemiptera	None	0.04%	5.0
Candidatus Moranella endobia Species-level Match	RISB1588	Planococcus citri Order: Hemiptera	None	0.03%	5.0
Lactiplantibacillus plantarum Species-level Match	RISB0608	Drosophila melanogaster Order: Diptera	None	0.02%	5.0
Arsenophonus nasoniae Species-level Match	RISB0366	Pachycrepoideus vindemmiae Order: Hymenoptera	None	0.02%	5.0
Rickettsia typhi Species-level Match	RISB1906	Bemisia tabaci Order: Hemiptera	None	0.02%	5.0
Gilliamella apicola Species-level Match	RISB1945	Apis cerana Order: Hymenoptera	None	0.01%	5.0
Candidatus Liberibacter asiaticus Species-level Match	RISB0750	Diaphorina citri Order: Hemiptera	None	0.01%	5.0
Lactobacillus apis Species-level Match	RISB1556	Apis florea Order: Hymenoptera	None	0.01%	5.0
Erwinia amylovora Species-level Match	RISB0403	Anaphes nitens Order: Hymenoptera	None	0.01%	5.0
Candidatus Steffania adelgidicola Species-level Match	RISB2278	Adelges nordmannianae/piceae Order: Hemiptera	None	0.01%	5.0
Candidatus Regiella	RISB1370	Sitobion avenae Order: Hemiptera	Regiella infection decreased the intrinsic rate of increase (rm) of aphids at 25 °C and 28 °C. However, at 31 °C, the effect of Regiella on the rm varied depending on the aphid genotype and density. Thus, the negative effects of this endosymbiont on its host were environmentally dependent.	0.01%	5.0
Acetobacter	RISB1865	Drosophila melanogaster Order: Diptera	The bacterial cells may thus be able to ameliorate the pH of the acidic region, by the release of weak bases.Additionally, the bacteria have a complex relationship with physiological processes which may affect ionic homeostasis in the gut, such as nutrition and immune function	0.01%	5.0
Bifidobacterium	RISB0174	Apis mellifera Order: Hymenoptera	Bifidobacterium provides complementary demethylation service to promote Gilliamella growth on methylated homogalacturonan, an enriched polysaccharide of pectin. In exchange, Gilliamella shares digestive products with Bifidobacterium, through which a positive interaction is established	0.01%	5.0
Candidatus Regiella	RISB1819	Sitobion avenae Order: Hemiptera	In R. insecticola-infected aphid lines, there were increases in plasticities for developmental times of first and second instar nymphs and for fecundity, showing novel functional roles of bacterial symbionts in plant-insect interactions.	0.01%	4.8
Apibacter	RISB0603	Apis cerana Order: Hymenoptera	The acquisition of genes for the degradation of the toxic monosaccharides potentiates Apibacter with the ability to utilize the pollen hydrolysis products, at the same time enabling monosaccharide detoxification for the host	0.03%	4.5
Candidatus Regiella	RISB1363	Sitobion avenae Order: Hemiptera	R. insecticola-infected aphids were more predated by the ladybird Hippodamia variegata irrespective of host plants and did not improve defences against coccinellid predators or metabolic rates on any host plants	0.01%	4.2
Xanthomonas	RISB0498	Xylocopa appendiculata Order: Hymenoptera	Xanthomonas strain from Japanese carpenter bee is effective PU-degradable bacterium and is able to use polyacryl-based PU as a nutritional source, as well as other types of PS-PU and PE-PU	0.03%	3.8
Acetobacter	RISB0961	Drosophila melanogaster Order: Diptera	The exist of Acetobacter had a balancing effect on food ingestion when carbohydrate levels were high in the warmer months, stabilizing fitness components of flies across the year.	0.01%	3.6
Candidatus Blochmanniella	RISB2542	Camponotus Order: Hymenoptera	Blochmannia provide essential amino acids to its host,Camponotus floridanus, and that it may also play a role in nitrogen recycling via its functional urease	0.38%	3.5
Candidatus Blochmanniella	RISB1827	Camponotus floridanus Order: Hymenoptera	a modulation of immune gene expression which may facilitate tolerance towards the endosymbionts and thus may contribute to their transovarial transmission	0.38%	3.5
Bifidobacterium	RISB0616	Spodoptera frugiperda Order: Lepidoptera	Strain wkB204 grew in the presence of amygdalin as the sole carbon source, suggesting that this strain degrades amygdalin and is not susceptible to the potential byproducts	0.01%	3.5
Pectobacterium	RISB1889	Pseudococcus longispinus Order: Hemiptera	a nested symbiotic arrangement, where one bacterium lives inside another bacterium,occurred in building the mosaic metabolic pathways seen in mitochondria and plastids	0.02%	3.4
Candidatus Blochmanniella	RISB2448	Camponotus floridanus Order: Hymenoptera	nutritional contribution of the bacteria to host metabolism by production of essential amino acids and urease-mediated nitrogen recycling	0.38%	3.1
Streptococcus	RISB2625	Galleria mellonella Order: Lepidoptera	suppress bacteria ingested with food by producing bacteriocin and by releasing a lysozyme like enzyme	0.70%	2.7
Shewanella	RISB1924	Anopheles gambiae Order: Diptera	may be related with mediating adaptation to different ecological niches or in shaping specific adult behaviors including mating	0.09%	2.6
Bartonella	RISB1673	Apis mellifera Order: Hymenoptera	a gut symbiont of insects and that the adaptation to blood-feeding insects facilitated colonization of the mammalian bloodstream	0.05%	2.6
Bacteroides	RISB0256	Leptocybe invasa Order: Hymenoptera	Differences in Male-Killing Rickettsia Bacteria between Lineages of the Invasive Gall-Causing Pest Leptocybe invasa	0.30%	2.6
Comamonas	RISB2021	Bactrocera dorsalis Order: Diptera	This group in the immature stages may be helping the insects to cope with oxidative stress by supplementing available oxygen.	0.03%	2.5
Yersinia	RISB0492	Cimex hemipterus Order: Hemiptera	the disruption of the abundant Yersinia possibly could be related to the enhanced susceptibility towards the insecticides	0.05%	2.5
Bacteroides	RISB0090	Hyphantria cunea Order: Lepidoptera	enhance the compatibility of invasive pests to new hosts and enable more rapid adaptation to new habitats.	0.30%	2.4
Streptococcus	RISB2624	Reticulitermes flavipes Order: Blattodea	can be broken down into substances such as carbon dioxide, ammonia and acetic acid	0.70%	2.3
Acetobacter	RISB0184	Drosophila melanogaster Order: Diptera	enhancing the brain levels of tyrosine decarboxylase 2 (Tdc2), which is an enzyme that synthesizes octopamine (OA)	0.01%	2.3
Blautia	RISB0091	Hyphantria cunea Order: Lepidoptera	enhance the compatibility of invasive pests to new hosts and enable more rapid adaptation to new habitats.	0.01%	2.1
Apilactobacillus	RISB0475	Apis mellifera Order: Hymenoptera	A. kunkeei alleviated acetamiprid-induced symbiotic microbiota dysregulation and mortality in honeybees	0.01%	2.1
Xanthomonas	RISB0217	Xylocopa appendiculata Order: Hymenoptera	strains biodegraded polyethylene terephthalate PET powder, broke it into its degradation products	0.03%	2.0
Streptococcus	RISB2604	Homona magnanima Order: Lepidoptera	influence the growth of Bacillus thuringiensis in the larvae	0.70%	1.9
Candidatus Zinderia	RISB2451	Clastoptera arizonana Order: Hemiptera	Zinderia had gene homologs for the production of tryptophan, methionine, and histidine	0.05%	1.8
Lachnospira	RISB2110	Blattella germanica Order: Blattodea	Hydrolyze polysaccharide; assist digestion; synthesize acetate, propionate, and butyrate	0.01%	1.8
Dysgonomonas	RISB1235	Hermetia illucens Order: Diptera	provides the tools for degrading of a broad range of substrates	0.01%	1.3
Paraclostridium	RISB0028	Sesamia inferens Order: Lepidoptera	degrade Chlorpyrifos and Chlorantraniliprole in vitro	0.17%	1.2
Pectobacterium	RISB0798	Pseudoregma bambucicola Order: Hemiptera	may help P. bambucicola feed on the stalks of bamboo	0.02%	1.1
Dickeya	RISB1086	Rhodnius prolixus Order: Hemiptera	supply enzymatic biosynthesis of B-complex vitamins	0.04%	1.1
Aeromonas	RISB2456	Bombyx mori Order: Lepidoptera	able to utilize the CMcellulose and xylan	0.03%	0.9
Cedecea	RISB1570	Bactrocera tau Order: Diptera	could attract male and female B. tau	0.01%	0.7
Aeromonas	RISB2086	Aedes aegypti Order: Diptera	axenic larvae cannot develop	0.03%	0.6
Priestia	RISB0839	Helicoverpa armigera Order: Lepidoptera	producing amylase	0.19%	0.5
Peribacillus	RISB1877	Aedes aegypti Order: Diptera	gut microbiome	0.15%	0.4
Candidatus Zinderia	RISB1640	Clastoptera arizonana Order: Hemiptera	Nitrogen-Fixing	0.05%	0.4
Comamonas	RISB1875	Aedes aegypti Order: Diptera	gut microbiome	0.03%	0.3
Helicobacter	RISB0662	Melanaphis bambusae Order: Hemiptera	None	0.14%	0.1
Myroides	RISB0626	Musca altica Order: Diptera	None	0.08%	0.1
Candidatus Profftia	RISB1664	Adelgidae Order: Hemiptera	None	0.07%	0.1
Metabacillus	RISB0902	Myzus persicae Order: Hemiptera	None	0.06%	0.1
Yersinia	RISB0407	Anaphes nitens Order: Hymenoptera	None	0.05%	0.1
Vagococcus	RISB0042	Aldrichina grahami Order: Diptera	None	0.05%	0.1
Apibacter	RISB0604	Apis cerana Order: Hymenoptera	None	0.03%	0.0
Micromonospora	RISB2033	Palomena viridissima Order: Hemiptera	None	0.02%	0.0
Pectobacterium	RISB1772	Muscidae Order: Diptera	None	0.02%	0.0
Candidatus Arthromitus	RISB2613	Multiple species Order: None	None	0.02%	0.0
Candidatus Phytoplasma	RISB1620	Cacopsylla pyricola Order: Hemiptera	None	0.02%	0.0
Bifidobacterium	RISB1944	Apis cerana Order: Hymenoptera	None	0.01%	0.0
Cedecea	RISB0504	Plutella xylostella Order: Lepidoptera	None	0.01%	0.0
Cupriavidus	RISB0694	Alydus tomentosus Order: Hemiptera	None	0.01%	0.0
Ralstonia	RISB0243	Spodoptera frugiperda Order: Lepidoptera	None	0.01%	0.0
Candidatus Vallotia	RISB1665	Adelgidae Order: Hemiptera	None	0.01%	0.0
Weeksella	RISB1265	Rheumatobates bergrothi Order: Hemiptera	None	0.01%	0.0