Amplicon

Our database features a diverse collection of 14,992 amplicon sequencing samples, representing various insect species from multiple geographical regions. Raw sequencing data were retrieved from NCBI, curated manually to ensure quality. Data preprocessing involved quality control and merging with Fastp, primer sequence trimming with Seqtk, and a standardized removal of 20 bp from the 5' ends of both forward and reverse reads. Taxonomic composition was analyzed using QIIME2 and Vsearch. Operational Taxonomic Units (OTUs) were classified with two workflows: QIIME2 utilized a pre-trained Greengenes classifier (gg_2022_10_backbone_full_length.nb.qza), while Vsearch employed the SINTAX algorithm with the Greengenes v13.5 reference database. Results were visualized using Krona for an interactive exploration of taxonomic distributions.

15035 Total Samples

Amplicon Types

Distribution of 16S and ITS sequences

Geographic Distribution

Sample distribution by continent

Amplicons

15035 records

Amplicon records associated with insect symbionts from public databases worldwide.

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Run Assay Type Classification Host Area Env Info Collection Year
SRR12656171 AMPLICON 16S Photuris sp. uncalculated - uncalculated 2017
SRR12656172 AMPLICON 16S Pyractomena borealis USA - North America 2017
SRR12656173 AMPLICON 16S Pyractomena borealis USA - North America 2017
SRR12656174 AMPLICON 16S Pyractomena borealis USA - North America 2017
SRR12656175 AMPLICON 16S Pyractomena borealis USA - North America 2017
SRR12656176 AMPLICON 16S Pyractomena borealis USA - North America 2017
SRR12656177 AMPLICON 16S Pyractomena borealis USA - North America 2017
SRR12656255 AMPLICON 16S Tenebrio sp. uncalculated - uncalculated 2016
SRR12656256 AMPLICON 16S Tenebrio sp. uncalculated - uncalculated 2016
SRR12656257 AMPLICON 16S Tenebrio sp. uncalculated - uncalculated 2016
SRR12656258 AMPLICON 16S Tenebrio sp. uncalculated - uncalculated 2016
SRR12656259 AMPLICON 16S Tenebrio sp. uncalculated - uncalculated 2016
SRR12656260 AMPLICON 16S Photuris sp. uncalculated - uncalculated 2017
SRR12656261 AMPLICON 16S Tenebrio sp. uncalculated - uncalculated 2016
SRR12656262 AMPLICON 16S Tenebrio sp. uncalculated - uncalculated 2016
SRR12656263 AMPLICON 16S Tenebrio sp. uncalculated - uncalculated 2016
SRR12656264 AMPLICON 16S Tenebrio sp. uncalculated - uncalculated 2016
SRR12656265 AMPLICON 16S Tenebrio sp. uncalculated - uncalculated 2016
SRR12685321 AMPLICON 16S Apis cerana China - Asia 2017
SRR12685322 AMPLICON 16S Apis cerana China - Asia 2017